breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 179 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,195,2481.C73.9% 188.5 / 67.9 65coding (101/837 nt)yeiGpredicted esterase
*REL6062,515,9090AC54.4% 37.9 / 78.4 57V260G (GTG→GGG) ypfIpredicted hydrolase
*REL606720,4640CG52.6% ‑1.1 / 24.8 19A22G (GCC→GGC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6064,180,2490TG48.9% 100.2 / 48.5 94G24G (GGT→GGGyjaHhypothetical protein
*REL6064,392,5560CT47.0% 48.3 / 93.7 100L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6061,854,4010AC45.8% 76.3 / 47.0 83K88N (AAA→AACyeaOhypothetical protein
*REL6063,774,9140CG41.5% 161.0 / 128.1 130A20P (GCC→CCC) yicJpredicted transporter
*REL6061,747,9030GT41.5% 461.3 / inf 376A8S (GCA→TCA) ydiLhypothetical protein
*REL6064,111,4290TG39.7% 92.8 / 10.4 58G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6062,144,2950AC38.0% 273.5 / 71.7 150G357G (GGT→GGGmrpantiporter inner membrane protein
*REL6063,096,3540AC37.5% 173.7 / 51.7 112V163G (GTG→GGG) sufIrepressor protein for FtsI
*REL6062,044,3570AC36.8% 286.5 / 90.3 163V284G (GTG→GGG) rfbDdTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase
*REL6064330TG36.8% 110.2 / 28.3 68V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6062,437,4060GT36.6% 83.2 / 21.3 41D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL606239,1630TG36.5% 226.0 / 79.6 137V58G (GTG→GGG) dnaQDNA polymerase III subunit epsilon
*REL6061,875,4830GC35.7% 515.9 / inf 362intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL606341,6780TG35.6% 149.4 / 51.7 87V171G (GTG→GGG) mhpA3‑(3‑hydroxyphenyl)propionate hydroxylase
*REL6062,685,2840TG35.3% 185.0 / 43.0 102V208G (GTG→GGG) ygaFpredicted enzyme
*REL6061,853,0670AC35.2% 156.0 / 75.6 105T56P (ACC→CCC) yeaNpredicted transporter
*REL6063,783,5650AC35.2% 162.9 / 35.5 91T744P (ACA→CCA) ECB_03521conserved hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 74979254 (1.330)15 (0.090)
+TCCCCGCCCCCT
7/126 15.3 6.0% intergenic (+60/+54) yabI/yabJ conserved inner membrane protein/thiamin transporter subunit
?REL606 2287059 = 311 (1.630)intergenic (+70/+58) ubiG/yfaL 3‑demethylubiquinone‑9 3‑methyltransferase/adhesin
* ? REL606 880528 =240 (1.260)6 (0.040) 6/122 15.7 3.4% intergenic (+17/+163) ybjK/ECB_00814 predicted DNA‑binding transcriptional regulator/conserved hypothetical protein
?REL606 = 904682 149 (0.960)intergenic (+77/+159) ECB_00851/ybjL protein similar to prophage P2 OGR protein/hypothetical protein
* ? REL606 1296152 =208 (1.090)3 (0.020) 3/128 18.4 1.7% coding (763/2676 nt) adhE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
?REL606 = 1296148 171 (1.050)coding (767/2676 nt) adhE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
* ? REL606 = 775941229 (1.200)4 (0.020) 3/134 18.8 2.4% coding (224/774 nt) modA molybdate transporter subunit
?REL606 1511277 = 125 (0.740)coding (184/696 nt) narW nitrate reductase 2 (NRZ), delta subunit (assembly subunit)
* ? REL606 = 1396823344 (1.800)3 (0.020) 3/144 19.5 1.2% intergenic (‑127/+68) fnr/ogt DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
?REL606 = 1397014 177 (0.970)coding (393/516 nt) ogt O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase