Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
14 |
24.7 |
1576304 |
96.5% |
1521133 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,409,177 |
G→A |
G91G (GGC→GGT) |
vacJ ← |
predicted lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,409,177 | 0 | G | A | 100.0%
| 79.3
/ NA
| 22 | G91G (GGC→GGT) | vacJ | predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/14); total (8/14) |
AACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGC > REL606/2409110‑2409249
|
naCCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCaa < 1:673345/75‑1 (MQ=255)
aCCGCGCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCaag < 2:620815/76‑1 (MQ=255)
cATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAagtag > 1:338833/1‑76 (MQ=255)
ccccAAAATGGTGTTTAGGAAAAAGAGGGAAAAGTGGACAATCCCCTGATAAGGGTGACCCTGCAAGTAGTAGTTa > 1:417935/1‑76 (MQ=255)
ccAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGCAGTTAAc < 1:491642/76‑1 (MQ=255)
cAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAAcc > 2:111028/1‑76 (MQ=255)
aaaaTGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCa > 2:539390/1‑76 (MQ=255)
aaTGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATc > 2:501338/1‑76 (MQ=255)
aaaaGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGttcttc < 2:757253/76‑1 (MQ=255)
aaGCGGGTAAAGTGGACCTTCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCaa > 2:711764/1‑76 (MQ=255)
aGCGGGTAAAGTGGACCATCTCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAg > 1:548569/1‑76 (MQ=255)
aGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAg < 1:501338/76‑1 (MQ=255)
aGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAg > 1:290448/1‑76 (MQ=255)
gggTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGtt < 1:539390/76‑1 (MQ=255)
gggTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGtt < 2:402769/76‑1 (MQ=255)
nggNCNACCCCCTTNTACGGGGTGCCGTGCAANAAGTAGTTCACCGTCACCGCAGGTTCCTCAAGGTTGCCAGTaa < 1:664751/75‑1 (MQ=255)
aCCATCCCCTGATAAGGGTCACCCTGCAAGTAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGt < 1:475162/76‑1 (MQ=255)
ccTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCaa > 1:419598/1‑76 (MQ=255)
gATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAAcc < 1:333765/76‑1 (MQ=255)
aaGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGtt < 2:460610/76‑1 (MQ=255)
aGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTa < 2:349033/76‑1 (MQ=255)
aGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTa < 2:74558/76‑1 (MQ=255)
ggTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCACAAACCGTTAcg < 2:6155/76‑1 (MQ=255)
gTCACCCTGCAAGAAGTAGTTAACCATCACCGCTGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACGGTTAcgc < 1:517079/76‑1 (MQ=255)
|
AACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGC > REL606/2409110‑2409249
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
