Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A83 F1 I1 R1
|
17 |
39.7 |
2446701 |
98.6% |
2412447 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,409,844 |
+C |
coding (102/933 nt) |
yfdC → |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,409,840 | 1 | . | C | 100.0%
| 56.8
/ NA
| 17 | L33P (CTC→CCC) | yfdC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/9); total (8/9) |
AACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CT‑CCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCA > REL606/2409765‑2409910
|
aaCACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑ct > 2:345033/1‑76 (MQ=255)
cgacgaAATTGAAGTTGAGCGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑ct‑ccccctc > 1:184539/1‑73 (MQ=255)
cgacgaAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑ct‑ccccctc > 1:184544/1‑73 (MQ=255)
cgaAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACCC‑CCCCTCCCg < 1:1152615/76‑1 (MQ=255)
cgaAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCg < 1:1152619/76‑1 (MQ=255)
gAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATg > 2:518771/1‑76 (MQ=255)
gAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATg < 2:1197872/76‑1 (MQ=255)
aGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGc > 2:989455/1‑76 (MQ=255)
cGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGa < 1:486017/76‑1 (MQ=255)
aaaaaGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCata > 1:1218839/1‑76 (MQ=255)
aaGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATcc < 1:1119030/76‑1 (MQ=255)
ggCAAAAAAATAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGAt < 2:778886/76‑1 (MQ=255)
aaaaaaTAGAAATAGATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTg > 2:1205065/1‑76 (MQ=255)
aaatagaaatagATGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGaaa < 1:364219/76‑1 (MQ=255)
aatagaaatagaTGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGaaaa < 1:745360/76‑1 (MQ=255)
tagaaatagaTGAAGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAag < 2:1152983/76‑1 (MQ=255)
gaTGAAGACCAA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGaa < 2:926703/76‑1 (MQ=255)
aGACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGa > 1:991118/1‑76 (MQ=255)
gACCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGAc > 1:181270/1‑76 (MQ=255)
aCCGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACg < 1:1211926/76‑1 (MQ=255)
ccGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGc > 1:15582/1‑76 (MQ=255)
cGA‑CTCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCa > 2:1028483/1‑76 (MQ=255)
|
AACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGA‑CT‑CCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCA > REL606/2409765‑2409910
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
