breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	474,211	Δ2 bp	coding (1094‑1095/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA	647,840	A→G	F353S (TTC→TCC)	mrdB ←	cell wall shape‑determining protein
MC JC	1,303,072	Δ1,756 bp		[oppD]–[cls]	[oppD], oppF, yciU, [cls]
RA	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I
RA	1,454,614	T→G	D722E (GAT→GAG)	hrpA →	ATP‑dependent helicase
JC	1,733,297	(CGTATGAA)1→2	coding (333/1413 nt)	pykF →	pyruvate kinase
RA	1,920,708	G→A	A151V (GCG→GTG)	znuA ←	high‑affinity zinc transporter periplasmic component
RA	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein
RA	2,336,054	G→A	A171V (GCA→GTA)	nuoM ←	NADH dehydrogenase subunit M
RA	2,358,409	T→A	I24N (ATC→AAC)	pta →	phosphate acetyltransferase
RA	2,409,844	+C	coding (102/933 nt)	yfdC →	predicted inner membrane protein
RA	3,762,741	A→T	K662I (AAA→ATA)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA	3,928,260	T→A	I15N (ATC→AAC)	rho →	transcription termination factor Rho
RA	4,202,203	Δ1 bp	coding (357/825 nt)	iclR ←	DNA‑binding transcriptional repressor
JC JC	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	2032758–2032783	2056031–2055883	23101–23274	13 [12]	[12] 13	[manB]–[cpsG]	[manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,wzx,rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG]