breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,688,9900AC37.5% 45.0 / 12.3 32T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6062,631,7720AC35.5% 47.0 / 13.8 31intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6064,574,2520TG34.8% 30.0 / 12.2 23S5S (TCA→TCChpaXhypothetical 4‑hydroxyphenylacetate permease
*REL6063,492,0820TG34.3% 63.0 / 14.6 35V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6064,393,0610TG33.3% 45.3 / 12.2 27V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6062,448,0980TG30.8% 75.8 / 15.5 39F278V (TTT→GTT) nupCnucleoside (except guanosine) transporter
*REL6062,296,3290AC28.6% 104.4 / 22.2 49intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL606275,4180AC28.1% 58.7 / 11.4 32F528V (TTT→GTT) yagWpredicted receptor
*REL6063,691,8440TG27.8% 67.9 / 10.6 36N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL6063,983,7000TG27.8% 69.6 / 15.4 36T397P (ACC→CCC) ECB_03713conserved hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 71905930 (0.770)4 (0.120) 3/132 5.1 17.3% coding (75/762 nt) ybfD hypothetical protein
?REL606 = 1506281 12 (0.350)intergenic (+143/‑97) ECB_01417/ydcC hypothetical protein/hypothetical protein
* ? REL606 = 71889719 (0.480)4 (0.120) 3/132 5.1 15.5% intergenic (+43/‑88) yhhI/ybfD predicted transposase; b3484_1/hypothetical protein
?REL606 = 719050 27 (0.780)coding (66/762 nt) ybfD hypothetical protein
* ? REL606 = 71763136 (0.920)4 (0.120) 3/132 5.1 12.0% intergenic (+246/‑87) ECB_00664/yhhI hypothetical protein/predicted transposase; b3484_1
?REL606 = 719050 27 (0.780)coding (66/762 nt) ybfD hypothetical protein
* ? REL606 570024 =9 (0.230)4 (0.120) 3/132 5.1 18.6% intergenic (‑88/+591) yhhI/ECB_00526 predicted transposase/hypothetical protein
?REL606 = 719050 27 (0.780)coding (66/762 nt) ybfD hypothetical protein
* ? REL606 = 24272515 (0.380)4 (0.120) 3/132 5.1 16.6% intergenic (+343/‑88) ECB_00212/yhhI hypothetical protein/predicted transposase
?REL606 = 719050 27 (0.780)coding (66/762 nt) ybfD hypothetical protein