breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,688,990 | 0 | A | C | 37.5% | 45.0 / 12.3 | 32 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
* | REL606 | 2,631,772 | 0 | A | C | 35.5% | 47.0 / 13.8 | 31 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 4,574,252 | 0 | T | G | 34.8% | 30.0 / 12.2 | 23 | S5S (TCA→TCC) | hpaX | hypothetical 4‑hydroxyphenylacetate permease |
* | REL606 | 3,492,082 | 0 | T | G | 34.3% | 63.0 / 14.6 | 35 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 4,393,061 | 0 | T | G | 33.3% | 45.3 / 12.2 | 27 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 2,448,098 | 0 | T | G | 30.8% | 75.8 / 15.5 | 39 | F278V (TTT→GTT) | nupC | nucleoside (except guanosine) transporter |
* | REL606 | 2,296,329 | 0 | A | C | 28.6% | 104.4 / 22.2 | 49 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 275,418 | 0 | A | C | 28.1% | 58.7 / 11.4 | 32 | F528V (TTT→GTT) | yagW | predicted receptor |
* | REL606 | 3,691,844 | 0 | T | G | 27.8% | 67.9 / 10.6 | 36 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 3,983,700 | 0 | T | G | 27.8% | 69.6 / 15.4 | 36 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 719059 | 30 (0.770) | 4 (0.120) | 3/132 | 5.1 | 17.3% | coding (75/762 nt) | ybfD | hypothetical protein |
? | REL606 | = 1506281 | 12 (0.350) | intergenic (+143/‑97) | ECB_01417/ydcC | hypothetical protein/hypothetical protein | |||||
* | ? | REL606 | = 718897 | 19 (0.480) | 4 (0.120) | 3/132 | 5.1 | 15.5% | intergenic (+43/‑88) | yhhI/ybfD | predicted transposase; b3484_1/hypothetical protein |
? | REL606 | = 719050 | 27 (0.780) | coding (66/762 nt) | ybfD | hypothetical protein | |||||
* | ? | REL606 | = 717631 | 36 (0.920) | 4 (0.120) | 3/132 | 5.1 | 12.0% | intergenic (+246/‑87) | ECB_00664/yhhI | hypothetical protein/predicted transposase; b3484_1 |
? | REL606 | = 719050 | 27 (0.780) | coding (66/762 nt) | ybfD | hypothetical protein | |||||
* | ? | REL606 | 570024 = | 9 (0.230) | 4 (0.120) | 3/132 | 5.1 | 18.6% | intergenic (‑88/+591) | yhhI/ECB_00526 | predicted transposase/hypothetical protein |
? | REL606 | = 719050 | 27 (0.780) | coding (66/762 nt) | ybfD | hypothetical protein | |||||
* | ? | REL606 | = 242725 | 15 (0.380) | 4 (0.120) | 3/132 | 5.1 | 16.6% | intergenic (+343/‑88) | ECB_00212/yhhI | hypothetical protein/predicted transposase |
? | REL606 | = 719050 | 27 (0.780) | coding (66/762 nt) | ybfD | hypothetical protein |