Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A124 F1 I1 R1	18	228.8	14151462	95.4%	13500494	76.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,162,551	G→A	G566S (GGT→AGT)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,162,551	0	G	A	97.6%	1008.8 / 7.1	246	G566S (GGT→AGT)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (119/121); total (121/125)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 248 total aligned reads displayed.

ACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACT  >  REL606/4162478‑4162622

aCGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGt                                                                       <  1:1876787/76‑1 (MQ=255)

  gTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTcc                                                                     >  2:4676381/1‑76 (MQ=255)

  gTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTcc                                                                     >  2:5433444/1‑76 (MQ=255)

    gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGa                                                                   <  2:2623077/76‑1 (MQ=255)

    gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGa                                                                   <  1:6990231/76‑1 (MQ=255)

    gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAATCCGa                                                                   >  2:5037957/1‑76 (MQ=255)

     cAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGaa                                                                  >  2:7057449/1‑76 (MQ=255)

          ttCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGACGGTCCGAACATCg                                                             >  1:6917722/1‑76 (MQ=255)

           tCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCgg                                                            >  1:1196932/1‑76 (MQ=255)

            cGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGt                                                           <  2:3194043/76‑1 (MQ=255)

            cGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGt                                                           >  1:2736025/1‑76 (MQ=255)

            cGAAGTGCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGt                                                           <  2:4857457/76‑1 (MQ=255)

             gAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTc                                                          >  2:2502376/1‑76 (MQ=255)

              aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt                                                         >  2:6186142/1‑76 (MQ=255)

              aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt                                                         <  2:3862835/76‑1 (MQ=255)

              aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt                                                         >  1:4495506/1‑76 (MQ=255)

              aaGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCt                                                         >  1:229431/1‑76 (MQ=255)

                 ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACNCNTGAAAGTCCGAACATCGGTCTGAt                                                      <  1:173432/76‑1 (MQ=255)

                 ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGAt                                                      <  2:4163699/76‑1 (MQ=255)

                   cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCa                                                    >  1:3025383/1‑76 (MQ=255)

                   cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCa                                                    >  1:2419446/1‑76 (MQ=255)

                    gagaCGTGCCCCCGACTCACGACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa                                                   <  2:2731509/76‑1 (MQ=255)

                    gagaCGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa                                                   <  1:647393/76‑1 (MQ=255)

                    gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa                                                   <  1:2810879/76‑1 (MQ=255)

                    gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCaa                                                   <  1:5015668/76‑1 (MQ=255)

                      gaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAAct                                                 >  2:165147/1‑76 (MQ=255)

                        cGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActct                                               <  2:5290670/76‑1 (MQ=255)

                        cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActct                                               >  1:4237525/1‑76 (MQ=255)

                         gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActctc                                              >  1:5537366/1‑76 (MQ=255)

                          tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAActctct                                             >  2:173607/1‑76 (MQ=255)

                               ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCg                                        >  1:518067/1‑76 (MQ=255)

                               ccGACGCACGACGGTCGCGTATGTCCAATCGAAACCCCGGAAAGGCCCAACATCGGGCGGAGCAACGCTCTGTCCg                                        <  1:1303802/76‑1 (MQ=255)

                                cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                       >  2:1143219/1‑76 (MQ=255)

                                cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                       >  1:4292848/1‑76 (MQ=255)

                                cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                       <  2:5678738/76‑1 (MQ=255)

                                 gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtg                                      <  2:6834012/76‑1 (MQ=255)

                                  aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                     <  2:4421377/76‑1 (MQ=255)

                                  aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                     <  2:3905385/76‑1 (MQ=255)

                                   cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa                                    >  2:5631342/1‑76 (MQ=255)

                                   cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa                                    >  2:3417670/1‑76 (MQ=255)

                                   cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa                                    >  1:1503780/1‑76 (MQ=255)

                                    tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                   >  1:5907647/1‑76 (MQ=255)

                                    tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                   <  2:629640/76‑1 (MQ=255)

                                    tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                   >  2:3480119/1‑76 (MQ=255)

                                     cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                  <  2:195718/76‑1 (MQ=255)

                                     cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                  >  1:652124/1‑76 (MQ=255)

                                     cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                  >  1:1536384/1‑76 (MQ=255)

                                     cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                  >  1:3660433/1‑76 (MQ=255)

                                      aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc                                 >  2:1815971/1‑76 (MQ=255)

                                       cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                <  1:6239798/76‑1 (MQ=255)

                                       cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                >  2:6692774/1‑76 (MQ=255)

                                       cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                <  2:4020156/76‑1 (MQ=255)

                                       cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCCCTGTCCGTGTACGca                                <  2:4001861/76‑1 (MQ=255)

                                        tACGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcac                               >  1:3522368/1‑76 (MQ=255)

                                          cgatCGCGTATGTCCAATCGAAACCCCTGGAAGTCCGAACATCTGTCTGATCAACTCTCTGTCCGTGTACGCACAg                             <  2:2219513/73‑1 (MQ=255)

                                          cGGTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAg                             >  2:5113757/1‑76 (MQ=255)

                                           ggTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa                            <  2:2316183/76‑1 (MQ=255)

                                            gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                           <  2:554503/76‑1 (MQ=255)

                                            gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                           <  1:107063/76‑1 (MQ=255)

                                            gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                           <  1:5046354/76‑1 (MQ=255)

                                            gTCGCGTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                           <  1:2633864/76‑1 (MQ=255)

                                              cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                         >  1:2098011/1‑76 (MQ=255)

                                              cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                         <  1:806499/76‑1 (MQ=255)

                                              cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                         <  2:6531122/76‑1 (MQ=255)

                                              cgcgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                         <  2:6886163/76‑1 (MQ=255)

                                                cgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                       <  1:1386018/76‑1 (MQ=255)

                                                cgTATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                       >  2:4724047/1‑76 (MQ=255)

                                                  tATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa                     >  2:1358114/1‑76 (MQ=255)

                                                  tATGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa                     >  1:4825814/1‑76 (MQ=255)

                                                   aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa                    <  2:4286086/76‑1 (MQ=255)

                                                   aTGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa                    >  1:4107086/1‑76 (MQ=255)

                                                    tGTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAAt                   >  1:1263384/1‑76 (MQ=255)

                                                     gTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                  <  1:6570290/76‑1 (MQ=255)

                                                     gTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                  <  2:3763186/76‑1 (MQ=255)

                                                     gTCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                  >  2:6787945/1‑76 (MQ=255)

                                                      tCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc                 <  2:835008/76‑1 (MQ=255)

                                                      tCCAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc                 <  1:195184/76‑1 (MQ=255)

                                                       ccAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg                >  2:3672284/1‑76 (MQ=255)

                                                       ccAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg                <  2:6096790/76‑1 (MQ=255)

                                                        cAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg               <  1:6732691/76‑1 (MQ=255)

                                                        cAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg               <  2:6856074/76‑1 (MQ=255)

                                                        cAATCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg               <  1:3760545/76‑1 (MQ=255)

                                                           tCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt            <  2:2948120/76‑1 (MQ=255)

                                                           tCGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt            <  2:1536982/76‑1 (MQ=255)

                                                            cGAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc           >  1:3253552/1‑76 (MQ=255)

                                                             gAAACCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc          >  2:3371124/1‑76 (MQ=255)

                                                              aaaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCt         >  2:4984215/1‑76 (MQ=255)

                                                              aaaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt         <  2:4226790/76‑1 (MQ=255)

                                                               aaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt        <  2:6154468/76‑1 (MQ=255)

                                                               aaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt        <  2:6113203/76‑1 (MQ=255)

                                                               aaCCCCTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt        >  2:779583/1‑76 (MQ=255)

                                                                 ccccTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCtttc      >  1:5621077/1‑74 (MQ=255)

                                                                  cccTGAAAGGCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag     <  1:3470460/76‑1 (MQ=255)

                                                                   ccTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTgaga    >  1:6347365/1‑76 (MQ=255)

                                                                    cTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc   <  1:4343348/76‑1 (MQ=255)

                                                                    cTGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc   >  1:2904863/1‑76 (MQ=255)

                                                                     tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt  <  1:5113757/76‑1 (MQ=255)

                                                                     tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt  <  1:4167236/76‑1 (MQ=255)

                                                                     tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt  <  1:6094685/76‑1 (MQ=255)

                                                                     tGAAAGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt  >  1:6823537/1‑76 (MQ=255)

ACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACT  >  REL606/4162478‑4162622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A