Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A84 F1 I1 R1
|
17 |
47.9 |
3074446 |
96.0% |
2951468 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,555,895 |
C→A |
P277Q (CCA→CAA) |
yhhI → |
predicted transposase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,555,895 | 0 | C | A | 96.8%
| 83.9
/ ‑5.2
| 31 | P277Q (CCA→CAA) | yhhI | predicted transposase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (18/12); total (19/12) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGAT > REL606/3555822‑3555961
|
gAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAg > 2:956628/1‑76 (MQ=21)
gAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAg < 2:272191/76‑1 (MQ=21)
aaTGGAAAGGGCTGAAGAATTTATGCGTGGCAGTCTCCTTTCGGTCCAT‑ATAGCAGAACAAAAGAAAGAGCAAGaa > 2:127674/1‑76 (MQ=11)
aaTGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGa > 2:1500282/1‑76 (MQ=21)
aTGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGaa > 1:1318224/1‑76 (MQ=21)
aTGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGaa < 1:980107/76‑1 (MQ=21)
aTGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGaa > 1:422549/1‑76 (MQ=21)
tGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGaaa > 1:911350/1‑76 (MQ=21)
ggAAAGGGCTGCAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAAt > 1:395121/1‑76 (MQ=21)
gAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATg > 1:1496160/1‑76 (MQ=21)
gAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATg < 1:1066418/76‑1 (MQ=21)
aGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACg < 2:1519963/76‑1 (MQ=21)
gCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTc < 2:1318906/76‑1 (MQ=21)
cTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCa > 2:183397/1‑76 (MQ=21)
tGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAg < 2:1201997/76‑1 (MQ=17)
tGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAg < 2:848264/76‑1 (MQ=17)
gaagaaATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGa < 1:305794/76‑1 (MQ=14)
gTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTctgctg > 2:547957/1‑76 (MQ=21)
tttCGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTaa < 2:1074477/76‑1 (MQ=21)
cGGTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCg > 2:1444112/1‑76 (MQ=21)
gTCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCt > 2:287747/1‑76 (MQ=25)
tCCATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTg < 1:183985/76‑1 (MQ=255)
ccATAATAGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTga > 2:1532099/1‑76 (MQ=255)
aataGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGaaa > 1:1335480/1‑76 (MQ=255)
aataGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGaaa > 2:317337/1‑76 (MQ=255)
aataGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGaaa < 2:788444/76‑1 (MQ=255)
ataGCAGAACAAAAGAAAGAGCCAGAAATGACGGGCAGATATTATATCAGCTCTGCTGATTTAACCGCTGAGAAAt > 2:469062/1‑76 (MQ=255)
taGCAGAACAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAAtt > 1:897977/1‑76 (MQ=255)
aCAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCcaca > 2:1446308/1‑76 (MQ=255)
aCAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCcaca > 2:1305047/1‑76 (MQ=255)
cAAAAGAAAGAGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAg < 1:280908/76‑1 (MQ=255)
agaaagaGCAAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGAt > 2:478058/1‑76 (MQ=255)
|
GAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGAT > REL606/3555822‑3555961
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
