Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A61 F1 I1 R1
|
19 |
50.7 |
3232285 |
96.8% |
3128851 |
74.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,259,207 |
G→A |
A33V (GCG→GTG) |
glmM ← |
phosphoglucosamine mutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,259,207 | 0 | G | A | 96.3%
| 198.0
/ ‑2.8
| 54 | A33V (GCG→GTG) | glmM | phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (27/25); total (28/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
ATATAGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATC > REL606/3259135‑3259277
|
atatAGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCcacg > 1:392352/1‑75 (MQ=255)
atatAGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCcacg > 2:628039/1‑75 (MQ=255)
tataGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACgg < 2:1128952/75‑1 (MQ=255)
tataGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACgg > 2:1171209/1‑75 (MQ=255)
tataGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACgg > 2:1462269/1‑75 (MQ=255)
tataGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACgg < 2:820490/75‑1 (MQ=255)
tataGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACgg > 1:742042/1‑75 (MQ=255)
aGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGccc > 1:1159877/1‑75 (MQ=255)
aGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGccc > 1:439850/1‑75 (MQ=255)
gCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCGCGGCCCa > 1:1357850/1‑75 (MQ=255)
ccAGAAATCCGCGTGTCTTTCCAAATAATGTTCTTCCGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCaa < 1:893568/75‑1 (MQ=255)
aGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAAcc > 2:14982/1‑75 (MQ=255)
aGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAAcc > 1:1242394/1‑75 (MQ=255)
aGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAAcc > 1:1220719/1‑75 (MQ=255)
aGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAAcc > 1:1164048/1‑75 (MQ=255)
aTACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGc > 1:332161/1‑75 (MQ=255)
tACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCt > 2:338682/1‑75 (MQ=255)
aCGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCCGCtt > 2:1155131/1‑75 (MQ=255)
aCGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCACCtt > 2:1507915/1‑75 (MQ=255)
gcgTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTaa < 1:881521/75‑1 (MQ=255)
cgTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAg < 2:462579/75‑1 (MQ=255)
gtgtCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGc < 2:852087/75‑1 (MQ=255)
tgtCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGca > 1:587720/1‑75 (MQ=255)
tCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGcaca > 1:1131089/1‑75 (MQ=255)
tCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGcaca > 2:526773/1‑75 (MQ=255)
cTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACaa < 1:1287611/75‑1 (MQ=255)
ttACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACaaaa > 1:1345345/1‑75 (MQ=255)
cAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAg < 2:1360641/75‑1 (MQ=255)
aataatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAgg < 1:1462270/75‑1 (MQ=255)
aataatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAgg > 1:1582133/1‑75 (MQ=255)
ataatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTAGCCACGGCCCAACCCAGCtt > 2:304768/1‑59 (MQ=255)
ataatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCtt < 1:304768/59‑1 (MQ=255)
aatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGgtgt < 1:645924/75‑1 (MQ=255)
aatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGgtgt < 1:1333844/75‑1 (MQ=255)
aatGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGgtgt > 2:1573082/1‑75 (MQ=255)
atGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGtgtg < 1:515217/75‑1 (MQ=255)
tGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGa < 1:338682/75‑1 (MQ=255)
gATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGAt > 1:908327/1‑75 (MQ=255)
tCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGTCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCg < 2:1323282/75‑1 (MQ=255)
cTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCgg > 1:1299228/1‑75 (MQ=255)
tACGGGAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGgcg > 1:1474165/1‑75 (MQ=255)
gggAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCAt > 1:487222/1‑75 (MQ=255)
ggAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATc > 2:524476/1‑75 (MQ=255)
gAGCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATcc < 2:1035980/75‑1 (MQ=255)
gCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATcccc < 1:1398066/75‑1 (MQ=255)
gCCGTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATcccc > 2:1241956/1‑75 (MQ=255)
gTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTac < 2:112418/75‑1 (MQ=255)
gTGGCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTac > 1:316547/1‑75 (MQ=255)
ggCGCGCCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTacac < 1:1495635/75‑1 (MQ=255)
cgcgcCAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGa < 1:188162/75‑1 (MQ=255)
ccAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCAc < 2:1535295/75‑1 (MQ=255)
ccAGCACTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCAc < 2:1158987/75‑1 (MQ=255)
aCTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATc < 1:1608809/75‑1 (MQ=255)
cTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCCTCCCCTACACGACCACGAATcc < 2:1315879/75‑1 (MQ=255)
cTTTACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATcc < 1:100556/75‑1 (MQ=255)
ttACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCa < 2:12435/75‑1 (MQ=255)
tACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCAt < 1:429282/75‑1 (MQ=255)
tACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCAt < 1:1405231/75‑1 (MQ=255)
tACCCACGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCAt > 1:167262/1‑75 (MQ=255)
cccccCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATc < 1:698254/74‑1 (MQ=255)
|
ATATAGCCAGAAATACGCGTGTCTTTACCAATAATGATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATC > REL606/3259135‑3259277
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
