Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
15 |
35.6 |
2322208 |
94.1% |
2185197 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,164,563 |
C→A |
N1236K (AAC→AAA) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,164,563 | 0 | C | A | 100.0%
| 113.6
/ NA
| 31 | N1236K (AAC→AAA) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (15/16); total (15/16) |
TGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTG > REL606/4164490‑4164632
|
tGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAaaca < 1:576775/76‑1 (MQ=255)
tACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAaacacc > 2:565222/1‑76 (MQ=255)
tACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAaacacc > 2:1099522/1‑76 (MQ=255)
aCGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCt < 1:669244/76‑1 (MQ=255)
cGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTg < 1:295638/76‑1 (MQ=255)
cGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTg < 1:650399/76‑1 (MQ=255)
aTGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGt > 1:712736/1‑76 (MQ=255)
ggTCGCAGTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTcg > 1:721467/1‑76 (MQ=255)
ggTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTcg < 1:847002/76‑1 (MQ=255)
gCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTcgacga > 2:288621/1‑76 (MQ=255)
cTGGTGAACAGTTCGAGCGTCCGGTAACCCTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACaa < 1:1044676/76‑1 (MQ=255)
ggTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGa < 2:566017/76‑1 (MQ=255)
gAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGc > 2:742422/1‑76 (MQ=255)
gTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCAcgcg < 1:502093/76‑1 (MQ=255)
cGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCa < 2:632790/76‑1 (MQ=255)
gttcGGTAACCGTTGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCAc < 1:537117/73‑1 (MQ=255)
ttGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAg < 1:157211/76‑1 (MQ=255)
tGGTTACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGc > 1:666055/1‑76 (MQ=255)
nttACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCt < 1:979994/75‑1 (MQ=255)
ttACATGTACATGCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTg > 1:326746/1‑76 (MQ=255)
tgacatgtacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCCTTCCACCGGTTCTTACAGCCTg > 1:835620/3‑76 (MQ=255)
tacatgtacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTgg > 1:951660/1‑76 (MQ=255)
acatgtacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt > 1:587652/1‑76 (MQ=255)
catgtacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGtt > 2:1139624/1‑76 (MQ=255)
catgtacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGtt > 1:314298/1‑76 (MQ=255)
tgtacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTAc > 1:47897/1‑76 (MQ=255)
tacatgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTc < 2:431195/76‑1 (MQ=255)
catgCTGAAACTGAAACATCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcag < 1:883280/76‑1 (MQ=255)
atgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTANTcagc > 2:929166/1‑76 (MQ=255)
tgCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcagca < 2:326746/76‑1 (MQ=255)
gCTGAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTATTACAGCCTGGTTACTcagcag > 1:798315/1‑76 (MQ=255)
ctgaaactgaaaCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCagcagc > 1:2578/1‑76 (MQ=255)
gAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCg < 1:716763/76‑1 (MQ=255)
gAAACTGAAACACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTCACTACAGCCTGGTTACTCAGCAGCCg > 2:847376/1‑76 (MQ=255)
acggAAACACCTGGTCGACGTNAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTg < 2:969150/73‑1 (MQ=255)
|
TGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTG > REL606/4164490‑4164632
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
