Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A45 F1 I1 R1
|
14 |
53.1 |
3307936 |
97.7% |
3231853 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,164,583 |
T→G |
M1243R (ATG→AGG) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,164,583 | 0 | T | G | 100.0%
| 196.8
/ NA
| 47 | M1243R (ATG→AGG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (25/22); total (25/22) |
AGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGG > REL606/4164514‑4164655
|
aGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCAcgc < 1:11854/76‑1 (MQ=255)
aGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCAcgc < 1:601782/76‑1 (MQ=255)
aGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCAcgc < 1:273784/76‑1 (MQ=255)
cGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGt > 2:448738/1‑76 (MQ=255)
gAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGtt > 2:1065591/1‑76 (MQ=255)
gAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGtt < 2:1492242/76‑1 (MQ=255)
gTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCAc > 2:1098756/1‑76 (MQ=255)
tCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCAcc < 1:1108742/76‑1 (MQ=255)
ccGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCg < 1:1427727/76‑1 (MQ=255)
ccGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCg > 1:777840/1‑76 (MQ=255)
ccGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCg < 2:900250/76‑1 (MQ=255)
ggTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGt > 2:1042104/1‑76 (MQ=255)
gTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGtt < 2:1415431/76‑1 (MQ=255)
tAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTGCCACCGGTTc > 1:59222/1‑76 (MQ=255)
aaCCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCt < 2:970953/76‑1 (MQ=255)
aCCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCtt < 1:556179/76‑1 (MQ=255)
gTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACa < 1:1450218/76‑1 (MQ=255)
ttGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAg > 1:1084304/1‑76 (MQ=255)
tGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGc > 2:1410284/1‑76 (MQ=255)
ttACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTg > 1:1356505/1‑76 (MQ=255)
tacatgtacatgCTGAAACTGANCCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTgg > 2:217877/1‑76 (MQ=255)
acatgtacatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt > 2:409396/1‑76 (MQ=255)
acatgtacatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt > 1:793756/1‑76 (MQ=255)
gtacatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACt > 1:577327/1‑76 (MQ=255)
acatgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTca < 2:1280892/76‑1 (MQ=255)
catgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcag < 2:48199/76‑1 (MQ=255)
catgCTGAAACTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcag > 2:391130/1‑76 (MQ=255)
gAAACTGAACCACCTGGTCGACAACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCg > 1:616082/1‑76 (MQ=255)
cTGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTgg < 2:807361/76‑1 (MQ=255)
tGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTggg > 1:981836/1‑76 (MQ=255)
tGAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTggg > 1:912977/1‑76 (MQ=255)
gAACCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGggt > 2:1520466/1‑76 (MQ=255)
aaCCACCTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTgggcg > 2:171829/1‑74 (MQ=255)
caccTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTa < 1:1368175/76‑1 (MQ=255)
cccTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTaa < 2:402333/75‑1 (MQ=255)
ccTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAg < 1:512280/76‑1 (MQ=255)
cTGGTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAgg < 1:1488213/76‑1 (MQ=255)
tGGTCGACGACAAGAGGCACGCGCGTTCCACCGGCTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGc > 1:140327/1‑76 (MQ=255)
ggTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGca < 2:1088626/76‑1 (MQ=255)
gTCGACGACAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGcac < 1:1317138/76‑1 (MQ=255)
cgacgaCAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAg < 2:1432558/76‑1 (MQ=255)
gacgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGt > 1:547756/1‑76 (MQ=255)
gacgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGt > 1:1281375/1‑76 (MQ=255)
gacgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGt > 1:1392749/1‑76 (MQ=255)
acgacAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGtt > 2:796534/1‑76 (MQ=255)
acAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCgg < 2:981836/76‑1 (MQ=255)
acAAGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCgg > 1:417818/1‑76 (MQ=255)
aaGAGGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCggtg < 2:1622196/76‑1 (MQ=255)
agagGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCggtgg > 2:208932/1‑76 (MQ=255)
|
AGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGG > REL606/4164514‑4164655
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
