Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
14 |
23.9 |
1506608 |
98.1% |
1477982 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,327,115 |
A→G |
I252T (ATT→ACT) |
mreC ← |
cell wall structural complex MreBCD transmembrane component MreC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,327,115 | 0 | A | G | 100.0%
| 96.4
/ NA
| 24 | I252T (ATT→ACT) | mreC | cell wall structural complex MreBCD transmembrane component MreC |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/17); total (7/17) |
CCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAA > REL606/3327043‑3327185
|
ccGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTca < 1:579588/76‑1 (MQ=255)
gATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGtata < 2:166402/76‑1 (MQ=255)
cTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAgc > 2:597656/1‑76 (MQ=255)
ccccccACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAgcgcg < 2:94143/76‑1 (MQ=255)
ccACAGCAGCAGCAGATAACCCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTgg > 1:10543/1‑76 (MQ=255)
acaGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGt < 2:486343/76‑1 (MQ=255)
cagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCg < 1:139912/76‑1 (MQ=255)
cagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCg > 1:192362/1‑76 (MQ=255)
gcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGttt < 2:403301/76‑1 (MQ=255)
cagATAACGCAAGCGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGtttt < 2:555768/76‑1 (MQ=255)
agATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTa < 1:740931/76‑1 (MQ=255)
agATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTa < 1:339395/76‑1 (MQ=255)
agATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTa < 2:309568/76‑1 (MQ=255)
cAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAagag < 2:570202/76‑1 (MQ=255)
aCGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACa < 1:275364/76‑1 (MQ=255)
cGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACaa < 1:525363/76‑1 (MQ=255)
gCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACcgc > 1:556337/1‑76 (MQ=255)
cTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCgg > 2:369603/1‑76 (MQ=255)
cTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCgg > 1:173670/1‑76 (MQ=255)
gCAGTCGGACGCGCCTGAGTCACAGTATAATCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGAt < 2:47806/76‑1 (MQ=255)
gCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGAt < 1:694716/76‑1 (MQ=255)
aGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAg > 2:366836/1‑76 (MQ=255)
cGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGcca < 2:78033/76‑2 (MQ=255)
ccTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGaa < 2:42870/76‑1 (MQ=255)
|
CCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAA > REL606/3327043‑3327185
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
