Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A26 F1 I1 R1
|
14 |
45.0 |
2861610 |
96.8% |
2770038 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,944 |
C→T |
A243V (GCG→GTG) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,944 | 0 | C | T | 98.2%
| 200.4
/ ‑3.6
| 55 | A243V (GCG→GTG) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (25/29); total (25/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 |
ATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCAT > REL606/3928871‑3929016
|
aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTgg > 2:988066/1‑76 (MQ=255)
aTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTgg < 1:640681/76‑1 (MQ=255)
tGCAGCGTCTGGTAAAAGGAGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGCTGTGGa > 1:760788/1‑76 (MQ=255)
cAGCGTCTGGTAANAGGTGAAGTTGTTGCTTCTACGTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGaaa < 2:1305100/76‑1 (MQ=255)
cGTCTGGTAAAAGGTGAAGTTGTTGTTTCTAGCTTTGACTNNCCCGCATCTCNNCGCGTTGAGGTTGTAGAAATgg > 1:1283499/1‑76 (MQ=255)
gTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGt > 1:315067/1‑76 (MQ=255)
tCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTg < 2:1309644/76‑1 (MQ=255)
tCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTg < 2:1061557/76‑1 (MQ=255)
ggTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATc < 2:676184/76‑1 (MQ=255)
ggTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATc < 2:585289/76‑1 (MQ=255)
ggTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTAGCCACGTTCAGGTTGTGGAAACGGTGATc > 1:72674/1‑76 (MQ=255)
gTAAAATGTGAAATTGTTGCTACTGCCTATGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCg < 2:242632/76‑1 (MQ=255)
tAAAAGGTGAAGTAGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCga > 1:101411/1‑76 (MQ=255)
aaaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCgag > 1:1241360/1‑76 (MQ=255)
aaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCgaga < 2:288625/76‑1 (MQ=255)
aaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGaa > 2:1230602/1‑76 (MQ=255)
ggTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGCTCAGGTTGTGGAAATGGTGATCGAGAAgg > 1:311099/1‑76 (MQ=255)
gTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGc < 1:539290/76‑1 (MQ=255)
gTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGc > 1:797522/1‑76 (MQ=255)
gAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCa < 2:1378822/76‑1 (MQ=255)
aaGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCaa < 1:623248/76‑1 (MQ=255)
aGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCaaa > 1:642109/1‑76 (MQ=255)
ttgttgCTTCTACCTTTGACGACCCCGCATCTCGCCACGTTCAGGTTGTGGAAAAGGTGATAGAGAAGGCCAAACg > 2:213117/1‑76 (MQ=255)
ttgttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACg < 1:216753/76‑1 (MQ=255)
tgttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGc > 1:410700/1‑76 (MQ=255)
gttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGcc > 1:581230/1‑76 (MQ=255)
gttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGcc > 1:687544/1‑76 (MQ=255)
ttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCt > 1:862432/1‑76 (MQ=255)
tgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTg < 2:1135845/76‑1 (MQ=255)
ttCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGtt > 1:462412/1‑76 (MQ=255)
ttCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGtt < 2:942920/76‑1 (MQ=255)
tCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGt < 2:722939/74‑1 (MQ=255)
tCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGt > 1:722939/1‑74 (MQ=255)
cTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGcac < 2:672780/76‑1 (MQ=255)
ttGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa < 2:1074890/76‑1 (MQ=255)
ttGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa < 1:213117/76‑1 (MQ=255)
ttGACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa < 2:872029/76‑1 (MQ=255)
tGACGAACCCGCATCTCGCNACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaag > 1:1334997/1‑76 (MQ=255)
gACGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaaga < 1:978387/76‑1 (MQ=255)
gACGAACCCGCATCTCGCCACGTACAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaaga < 1:788766/76‑1 (MQ=255)
aCGAACCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaagaa < 1:566495/76‑1 (MQ=255)
aaCCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaagaaaga < 1:1197196/76‑1 (MQ=255)
aCCCGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGAc > 2:647489/1‑76 (MQ=255)
cGCATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGtt < 2:427633/76‑1 (MQ=255)
cATCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTAt < 1:240766/76‑1 (MQ=255)
aTCTCGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGATTGAGCACACGAAAGACGTTATc > 1:1229869/1‑76 (MQ=255)
tctcGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCa < 1:113646/76‑1 (MQ=255)
tctcGCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCa < 2:601509/76‑1 (MQ=255)
gCCACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCt < 2:1378542/76‑1 (MQ=255)
cccACGTTCAGGTTGTGGAAATGGTGATGGAGAATGCCAAACGCCTGNTTAAGCACAAGAAAGACGTTATCATTCt > 1:124079/2‑76 (MQ=255)
cACGTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGc > 2:619913/1‑76 (MQ=255)
gTTCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCg < 2:6599/76‑1 (MQ=255)
tCAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGAc > 1:1051845/1‑76 (MQ=255)
cAGGTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACt > 1:1081383/1‑76 (MQ=255)
gTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCa < 2:760788/76‑1 (MQ=255)
gTTGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCa > 2:58992/1‑76 (MQ=255)
ttGTGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCAt > 1:583505/1‑76 (MQ=255)
|
ATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCAT > REL606/3928871‑3929016
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
