Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A80 F1 I1 R1
|
17 |
48.4 |
3161007 |
95.9% |
3031405 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,292,285 |
A→G |
K807E (AAG→GAG) |
gltB → |
glutamate synthase, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,292,285 | 0 | A | G | 100.0%
| 219.7
/ NA
| 56 | K807E (AAG→GAG) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/29); total (27/29) |
CGCATTGGTGGGGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGT > REL606/3292210‑3292355
|
cGCATTGGTGGGGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTa < 1:20674/76‑1 (MQ=255)
tggGGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATc > 2:611267/1‑76 (MQ=255)
gcgaGCTTTGAAGTCTGCCAGAAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGGGAGCCCATCAGcc < 2:468506/73‑1 (MQ=255)
gCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGcc < 2:458776/76‑1 (MQ=255)
gCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGcc < 2:978499/76‑1 (MQ=255)
gCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGcc < 2:935892/76‑1 (MQ=255)
gCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGcc < 2:46897/76‑1 (MQ=255)
cAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCa < 2:28874/76‑1 (MQ=255)
aGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAgg < 1:598284/76‑1 (MQ=255)
ttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAcggcgg > 2:1490600/1‑76 (MQ=255)
ttGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGggcgg < 1:1324525/76‑1 (MQ=255)
gAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGcc > 1:551117/1‑76 (MQ=255)
aGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCctg < 2:461385/76‑1 (MQ=255)
aGACTTCCACCAGGATTTGCTAAATCTGTCGAAGCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCctg < 2:531315/76‑1 (MQ=255)
tCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGaa < 2:1363411/76‑1 (MQ=255)
cagcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAAt < 1:998816/76‑1 (MQ=255)
agcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATa > 1:10567/1‑76 (MQ=255)
agcagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATa < 1:1120202/76‑1 (MQ=255)
cagGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACg > 1:917937/1‑76 (MQ=255)
gATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTcc > 2:1384523/1‑76 (MQ=255)
aTCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCa < 1:388541/76‑1 (MQ=255)
ctgctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCAcg < 1:1210907/76‑1 (MQ=255)
ctgctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCAcg > 2:1511064/1‑76 (MQ=255)
tgctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCAcgg > 2:489371/1‑76 (MQ=255)
tgctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCAcgg < 1:1213893/76‑1 (MQ=255)
gctgAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCAcggc > 2:160885/1‑76 (MQ=255)
tgAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCAcggcgg > 2:649728/1‑76 (MQ=255)
gAATCTGTCGAAGCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACggcggc < 2:848375/76‑1 (MQ=255)
gAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCTCAACAGCCAGGGCGGCCTGCTGAAATACGTCCACggcggc > 1:596188/1‑76 (MQ=255)
gAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACggcggc < 1:1071882/76‑1 (MQ=255)
gAATCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACggcggc > 1:686885/1‑76 (MQ=255)
aaTCTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGgcggcg > 2:455025/1‑76 (MQ=255)
cTGTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAAt > 1:1057061/1‑76 (MQ=255)
tGTCGAAACGGGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATa < 1:1468367/76‑1 (MQ=255)
gTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGGGAATAc > 1:1436211/1‑76 (MQ=255)
gTCGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATaa > 2:183165/1‑75 (MQ=255)
cGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCa < 2:1058283/76‑1 (MQ=255)
cGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCa < 1:1506352/76‑1 (MQ=255)
cGAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCa > 1:1279715/1‑76 (MQ=255)
gAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCAc > 2:1338746/1‑76 (MQ=255)
gAAACGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCAc < 1:1412429/76‑1 (MQ=255)
aaaCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCaag > 2:869342/1‑74 (MQ=255)
aaaCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACg > 2:888879/1‑76 (MQ=255)
aaaCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACg > 2:547171/1‑76 (MQ=255)
aaaCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACg < 1:235655/76‑1 (MQ=255)
aaCGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGc > 2:1040023/1‑76 (MQ=255)
cGTGCCTGGCTGGCGCGTGAGCCCATCCGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCt < 2:128471/76‑1 (MQ=255)
cGTGCCTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCt < 2:1573286/76‑1 (MQ=255)
ccTGGCTGGCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACaa < 2:306783/76‑1 (MQ=255)
ctggctggCGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAAc < 2:686884/76‑1 (MQ=255)
tggctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAAcc > 1:1214972/1‑76 (MQ=255)
ggctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGGCCACAAacc > 2:799207/1‑76 (MQ=255)
ggctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAAccc > 1:562152/1‑76 (MQ=255)
ggctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAAccc > 2:1091795/1‑76 (MQ=255)
ctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCgg < 1:1421802/76‑1 (MQ=255)
ctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCgg > 2:10329/1‑76 (MQ=255)
ctggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCgg > 2:319806/1‑76 (MQ=255)
ggcGCGTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGAc < 1:455026/76‑1 (MQ=255)
cgcgTGAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACgt < 1:1551642/76‑1 (MQ=255)
|
CGCATTGGTGGGGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGCCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTACAACCCGGACGT > REL606/3292210‑3292355
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
