Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F1 I1 R1
|
16 |
43.9 |
3070277 |
96.2% |
2953606 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,161,978 |
C→T |
P375S (CCG→TCG) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,161,978 | 0 | C | T | 100.0%
| 129.5
/ NA
| 37 | P375S (CCG→TCG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/23); total (14/23) |
ACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACCTGTCTG > REL606/4161908‑4162050
|
aCGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGtcgccg < 1:885075/76‑1 (MQ=255)
tgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGCCt > 2:29122/1‑76 (MQ=255)
tgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACt < 2:733541/76‑1 (MQ=255)
gtCGACCCAACTAACGACCGTCTTAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTc > 1:690759/1‑76 (MQ=255)
gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTc < 2:478062/76‑1 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTg < 2:825092/76‑1 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTg > 1:825093/1‑76 (MQ=255)
aCCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGa < 1:228791/76‑1 (MQ=255)
aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAagcag < 2:990919/76‑1 (MQ=255)
cGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGaa < 1:1246214/76‑1 (MQ=255)
gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCt > 2:600253/1‑76 (MQ=255)
gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:343806/76‑1 (MQ=255)
gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCt > 1:584949/1‑76 (MQ=255)
tCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTg < 1:968470/76‑1 (MQ=255)
cTGAGCGCACTGGTAGAAATCTCCCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGt < 1:957608/76‑1 (MQ=255)
cTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGt > 2:369029/1‑76 (MQ=255)
gAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTc < 2:345762/76‑1 (MQ=255)
gAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc < 2:268674/76‑1 (MQ=255)
aGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCTTGAAGCAGCGGAAAGCCTGTTCg < 2:1004537/76‑1 (MQ=255)
ggcACTGGAAGAAATCTACCGCATGAGGCGCCGTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag < 1:1032935/75‑1 (MQ=255)
cgcACTGGTAGAAATCTCCCGCATGATGCGCCCGGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag < 1:868222/76‑1 (MQ=255)
tGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCt > 2:308557/1‑76 (MQ=255)
ggTAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTg > 2:484698/1‑76 (MQ=255)
tAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGtt > 1:1062206/1‑76 (MQ=255)
tAGAAATCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGtt > 2:167892/1‑76 (MQ=255)
aaaTCTACCGCATGATGCTCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 1:207912/76‑1 (MQ=255)
aaaTCTACCGAATGGTGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGttctt < 1:1490414/76‑1 (MQ=255)
aTCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTtcttct < 2:1234545/76‑1 (MQ=255)
aTCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTtcttct > 1:739652/1‑76 (MQ=255)
aTCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTtcttct < 2:949909/76‑1 (MQ=255)
tCTACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTtctc < 1:397302/76‑1 (MQ=255)
tACCGCATGATGCGCCCTGGCGAGTCGCCGACTCGTGTAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCg < 2:346357/76‑1 (MQ=255)
gCATGATGCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGa > 1:1358684/1‑76 (MQ=255)
tgatgCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCg > 2:270685/1‑69 (MQ=255)
tgatgCGCCCTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCg < 1:270686/69‑1 (MQ=255)
cccTGGCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAc < 2:1212257/76‑1 (MQ=255)
ggCGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGt > 2:1406735/1‑76 (MQ=255)
cGAGTCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACCTGTCt < 1:372462/76‑1 (MQ=255)
gAGCCCCCGACTCCTGAAGCAGCAGAAAGCCTGTTCGAGAACATGTTCTTCTCCGAAGACCGTTATCACCTGTCTg < 2:1318942/76‑1 (MQ=255)
|
ACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACCTGTCTG > REL606/4161908‑4162050
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
