breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 430,954 G→A E4E (GAG→GAA)  lon → DNA‑binding ATP‑dependent protease La
RA 475,031 G→T A92E (GCG→GAG)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 649,985 C→G V273L (GTG→CTG)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,028,170 G→A R98* (CGA→TGA)  ECB_02828 ← Polysialic acid transport protein kpsM
RA 3,339,489 Δ1 bp intergenic (+31/‑55) fis → / → yhdJ DNA‑binding protein Fis/predicted methyltransferase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD–[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,991 (T)5→4 intergenic (‑192/‑131) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 4,160,666 T→G intergenic (+130/‑190) rplL → / → rpoB 50S ribosomal protein L7/L12/DNA‑directed RNA polymerase subunit beta
RA 4,161,978 C→T P375S (CCG→TCG)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,504,637 G→A intergenic (+381/‑344) insB‑25 → / → ECB_04162 b4563(b4576); IS1 protein insB/hypothetical protein
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2651895NA (NA)39 (0.850) 30/144 0.5 NA noncoding (670/1542 nt) rrsG 16S ribosomal RNA
?REL606 = 2774196 NA (NA)noncoding (1343/1343 nt) IS186 repeat region