Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A76 F1 I1 R1
|
13 |
52.2 |
3332858 |
96.5% |
3216207 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,304 |
C→T |
R30C (CGT→TGT) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,304 | 0 | C | T | 93.1%
| 198.9
/ 5.2
| 58 | R30C (CGT→TGT) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (21/33); total (22/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01 |
AATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCG > REL606/3928230‑3928376
|
aaTTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCg < 1:523229/76‑1 (MQ=255)
ttAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTa < 1:545649/76‑1 (MQ=255)
tAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTaa < 2:943987/76‑1 (MQ=255)
tAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTaa > 1:1503879/1‑76 (MQ=255)
tAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa > 1:869186/1‑76 (MQ=255)
aaaGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAg > 2:222701/1‑76 (MQ=255)
aaGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGc < 2:574665/76‑1 (MQ=255)
aGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCa < 1:1010200/76‑1 (MQ=255)
aaTACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAgg < 2:1548700/76‑1 (MQ=255)
aTACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGa > 1:388377/1‑76 (MQ=255)
tACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGAc > 2:1610773/1‑76 (MQ=255)
tACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGAc > 2:1020336/1‑76 (MQ=255)
gCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACatt > 1:140007/1‑76 (MQ=255)
ggTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACattatt > 2:796694/1‑76 (MQ=255)
gTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTAttt < 1:1133970/76‑1 (MQ=255)
gTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTAttt < 2:297855/76‑1 (MQ=255)
tttCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTAtttt > 1:427694/1‑76 (MQ=255)
ttCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGGATGTGTAAGCAGGAAAtttttttt > 2:1360605/1‑76 (MQ=255)
cTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGc < 2:167127/76‑1 (MQ=255)
tGAGCTGATCACTCTCGGCGAAAATATGGGCGTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGcc < 2:699918/76‑1 (MQ=255)
gAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCa > 1:684932/1‑76 (MQ=255)
gAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCa < 1:673915/76‑1 (MQ=255)
ngCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATATGTAAGCAGGACATTATTTTTGCCAt > 1:1357386/2‑76 (MQ=255)
aGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCAt > 2:481700/1‑76 (MQ=255)
tGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCt > 1:370277/1‑76 (MQ=255)
gATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTg > 1:334560/1‑76 (MQ=255)
cACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAag < 2:1632856/76‑1 (MQ=255)
ctctCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAagca < 2:154709/76‑1 (MQ=255)
tctcGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAagcag < 1:440788/76‑1 (MQ=255)
ctcGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAagcagc < 1:1533942/76‑1 (MQ=255)
tcGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAgcagca < 1:349020/76‑1 (MQ=255)
cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCa > 2:83665/1‑76 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAag > 1:765822/1‑76 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAag < 2:427694/76‑1 (MQ=255)
aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAag < 1:103083/76‑1 (MQ=255)
aaTATGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAaga < 1:1207234/76‑1 (MQ=255)
atatGGGGCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAagag < 2:1530420/76‑1 (MQ=255)
gggCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCg < 2:607600/76‑1 (MQ=255)
ggCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGa < 1:66139/76‑1 (MQ=255)
gCTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGaa < 2:775587/76‑1 (MQ=255)
cTGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAg < 2:899681/76‑1 (MQ=255)
tGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGa > 2:1514545/1‑76 (MQ=255)
tGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGa < 2:1121563/76‑1 (MQ=255)
tGGAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGa < 2:184987/76‑1 (MQ=255)
ggAAAACCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGat < 1:1374321/76‑1 (MQ=255)
aCCTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCtt < 1:1228618/76‑1 (MQ=255)
ccTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCttt < 2:659026/76‑1 (MQ=255)
cTGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTg > 1:1463519/1‑76 (MQ=255)
tGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTgg < 1:1529788/76‑1 (MQ=255)
tGGCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTgg > 1:1129976/1‑76 (MQ=255)
ggCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGt < 1:261605/76‑1 (MQ=255)
gCTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTg < 2:1478289/76‑1 (MQ=255)
cTCGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGa < 2:4274/76‑1 (MQ=255)
cGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATg > 1:442896/1‑76 (MQ=255)
cGTATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATg > 2:1417956/1‑76 (MQ=255)
gTATGTGTAAGCAGGACATTATTTTTGCCATTCTGAAGCAGCGCGAAAAGAGTGGCGAAGATATCTTTGGTGATgg < 2:1247504/76‑1 (MQ=255)
gTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATgg < 2:970987/76‑1 (MQ=255)
tATGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGc < 1:383118/76‑1 (MQ=255)
aTGTGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCg < 1:1422264/76‑1 (MQ=255)
|
AATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCG > REL606/3928230‑3928376
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
