Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
17 |
37.0 |
2377229 |
96.2% |
2286894 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
REL606 |
3,928,336 |
+AAG |
coding (120/1260 nt) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
REL606 |
= 3928332 | 0 (0.000) | 38 (1.070)
+GAA |
31/136 |
0.1 |
100% |
coding (116/1260 nt) |
rho |
transcription termination factor Rho |
| ? | REL606 |
3928333 = |
0 (0.000) | coding (117/1260 nt) |
rho |
transcription termination factor Rho |
GATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/3928258‑3928332
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGT > REL606/3928333‑3928405
|||
GATCACTCTCGGCGAAAATATGCGGCTGGAAAACCTGTCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTG < 1:862134/76‑1
ATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGA > 1:1113991/1‑76
TCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAA < 2:857071/76‑1
CACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAG > 1:1108989/1‑76
CACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAG > 1:48052/1‑76
ACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGA < 1:1150464/76‑1
CTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAA > 1:686591/1‑76
CTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGC > 1:84784/1‑76
CTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGC < 2:837086/76‑1
CGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAG < 1:323393/76‑1
GAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACG < 2:344022/76‑1
GAAAAGTTGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACG < 1:138355/76‑1
AAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGC < 2:38587/76‑1
ATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAA > 2:282088/1‑76
nGGGCTGGAAAACCTGGCTCGTATGCGTGAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGT < 1:1039278/75‑1
GGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTG < 1:389695/76‑1
GCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGC > 2:855489/1‑76
CTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCG < 2:970973/76‑1
TGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGA > 2:607146/1‑76
GGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGTCATCCTGAATAAGCAGCACGCAAAGAGTGGCGAA < 2:484731/76‑1
AACCTGGCTCGTATGCGTAAGCAAGACATTATTTTTGCCATCCTGATGAAGCAGCACGCAAAGAGTGGCGAAGAca < 1:1027364/76‑3
tCCTGGCTCGGATGCGTATGCAGGAGATTATTCGTGCCATCCTGTAGAAGCAGCACGCAAAGAGTGGCGAAGATAT < 1:1094109/75‑1
CTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGG < 2:815036/76‑1
AAGCAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTAC > 1:46446/1‑76
GCAGGACATTATTTTTGCCGTCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTG < 2:713010/76‑1
CAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGG > 2:25805/1‑76
CAGGACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGG > 2:654807/1‑76
ACATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGAT > 1:370839/1‑76
CATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATA < 2:778712/76‑1
ATTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAT < 2:388564/76‑1
TTATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATT < 2:556488/76‑1
TATTTTTGCCGTCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTG > 1:156261/1‑76
ATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGC > 2:714426/1‑76
ATTTTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGC < 2:84784/76‑1
TTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGG > 1:216767/1‑76
TTTGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGG > 1:438374/1‑76
TGCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGAT > 1:1109997/1‑76
GCCATCCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATG > 2:1098745/1‑76
CCTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTT > 1:87591/1‑76
CTGAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTG > 1:761104/1‑76
GAAGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGANTTGGT > 2:968056/1‑76
|||
GATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/3928258‑3928332
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGT > REL606/3928333‑3928405
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
N/A
