Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F1 I1 R1
|
18 |
50.6 |
3235256 |
96.4% |
3118786 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,272 |
C→T |
P806L (CCG→CTG) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,272 | 0 | C | T | 97.6%
| 150.4
/ ‑3.2
| 41 | P806L (CCG→CTG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/22); total (19/22) |
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 |
GCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCAC > REL606/4163201‑4163344
|
gCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTgg < 1:459697/76‑1 (MQ=255)
cTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGa > 2:932938/1‑76 (MQ=255)
tGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGaa < 2:624538/76‑1 (MQ=255)
cAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATgg > 2:767271/1‑76 (MQ=255)
cGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTAc > 1:108927/1‑76 (MQ=255)
ggTCCGTCCACCGACCTCGGTGAACTGGCGCTTNGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACa > 2:1364741/1‑76 (MQ=255)
ggTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACa < 1:1004606/76‑1 (MQ=255)
gtccgtccACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACaa < 2:572809/76‑1 (MQ=255)
gtccACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTc < 2:634603/76‑1 (MQ=255)
tccACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCg < 2:89851/76‑1 (MQ=255)
cACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGaa > 1:1285608/1‑76 (MQ=255)
gACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACt < 1:892909/76‑1 (MQ=255)
cTCGNTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGNTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCa < 1:197111/76‑1 (MQ=255)
cTCGGTGAACTGGCGCTTGGTCAGAACATGCGCNTAGCGTTCATGCTGTGGAATGGTTACAACTTCCAAGACTCCa > 2:1375897/1‑76 (MQ=255)
cGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATc > 1:1377893/1‑76 (MQ=255)
gAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCg > 1:1091813/1‑76 (MQ=255)
aaCTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGt > 2:1488271/1‑76 (MQ=255)
cTGNCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGANTGGTTACAACTTCGAAGACTCCATCCTCGTAt < 2:1512355/76‑1 (MQ=255)
tGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATc > 1:1157113/1‑76 (MQ=255)
gcgcTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCg < 2:870850/76‑1 (MQ=255)
gcgcTTGGTCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCg < 2:509876/76‑1 (MQ=255)
ttGGTCAGAACATGCGCGTAGCGTTCATGCTGTTGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCg > 1:1320909/1‑76 (MQ=255)
tCAGAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGtgtt > 1:76249/1‑76 (MQ=255)
aGAACATGCGCGTAGCGCTCATGCTGTGGAATGGTTACAACTTCGAAGACGCCATCCTCGTATCAGAGCGtgtngt > 2:1529568/1‑76 (MQ=255)
gAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCGATCCTCGTATCCGAGCGTgttgtt < 2:1172122/76‑1 (MQ=255)
gAACATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTgttgtt < 1:966892/76‑1 (MQ=255)
aaCATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTc < 1:825045/76‑1 (MQ=255)
aCATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCa > 2:128445/1‑76 (MQ=255)
cATGCGCGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAg < 2:488263/76‑1 (MQ=255)
gcgcGTAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGaa > 2:674113/1‑76 (MQ=255)
cgTAGCGTTCATGCTGTGCAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGAc < 2:823848/76‑1 (MQ=255)
tAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCg < 2:309585/76‑1 (MQ=255)
tAGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCg < 1:548122/76‑1 (MQ=255)
aGCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGt > 2:183859/1‑76 (MQ=255)
aGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGt > 2:320340/1‑76 (MQ=255)
gCGTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGtt < 2:401363/76‑1 (MQ=255)
gTTCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTc < 1:1264743/76‑1 (MQ=255)
tCATGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTcac < 1:1179962/76‑1 (MQ=255)
aTGCTGTGGAATGGTTACANCTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTcacca > 1:1516126/1‑76 (MQ=255)
tGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTcaccac < 2:1091905/76‑1 (MQ=255)
tGCTGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTcaccac > 1:631220/1‑76 (MQ=255)
|
GCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCAC > REL606/4163201‑4163344
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
