Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A127 F1 I1 R1
|
15 |
330.5 |
20417406 |
95.5% |
19498622 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,327,533 |
G→T |
R113S (CGT→AGT) |
mreC ← |
cell wall structural complex MreBCD transmembrane component MreC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,327,533 | 0 | G | T | 99.1%
| 1312.9
/ ‑3.5
| 328 | R113S (CGT→AGT) | mreC | cell wall structural complex MreBCD transmembrane component MreC |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (159/165); total (161/167) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 338 total aligned reads displayed.
ATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACT > REL606/3327460‑3327605
|
aTAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCa > 2:7096192/1‑76 (MQ=255)
tAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATACTGACTCAg > 2:7518628/1‑76 (MQ=255)
aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGc > 1:9167530/1‑76 (MQ=255)
aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGc > 2:4340793/1‑76 (MQ=255)
aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGc < 1:7473729/76‑1 (MQ=255)
aacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCggg < 2:2682110/76‑1 (MQ=255)
ttGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGaa < 2:4736382/76‑1 (MQ=255)
ttGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGaa < 2:6550654/76‑1 (MQ=255)
tGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAAc < 1:7102187/76‑1 (MQ=255)
aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAAccc < 1:3220947/76‑1 (MQ=255)
aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAAccc > 1:3073088/1‑76 (MQ=255)
tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCca < 2:1862606/76‑1 (MQ=255)
tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCca > 1:6212559/1‑76 (MQ=255)
tCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCca < 2:407015/76‑1 (MQ=255)
cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcag < 2:5125069/76‑1 (MQ=255)
cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcag > 2:8017740/1‑76 (MQ=255)
cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcag > 2:8700725/1‑76 (MQ=255)
gCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCcagc > 1:6235672/1‑76 (MQ=255)
cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagca < 1:9261838/76‑1 (MQ=255)
cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagca > 2:3584087/1‑76 (MQ=255)
cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagca > 1:1811269/1‑76 (MQ=255)
cTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACACAGCGGGGAACCcagca > 1:4564496/1‑76 (MQ=255)
tataAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagcag > 2:10042130/1‑76 (MQ=255)
tataAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCcagcag > 1:2101562/1‑76 (MQ=255)
aaGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTc > 1:2519273/1‑76 (MQ=255)
aGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTcg < 2:3703439/76‑1 (MQ=255)
aGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTcc > 2:3327571/1‑75 (MQ=255)
gATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTcgcg < 1:4874774/76‑1 (MQ=255)
aTCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCgcgc > 1:9780308/1‑76 (MQ=255)
tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCgcgcc > 1:667749/1‑75 (MQ=255)
tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCa < 1:10090469/76‑1 (MQ=255)
tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCa < 1:8885249/76‑1 (MQ=255)
tCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCa < 1:5283074/76‑1 (MQ=255)
cGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAg > 2:5334828/1‑76 (MQ=255)
cGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAg < 2:2189614/76‑1 (MQ=255)
tttAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGa < 1:2937597/75‑1 (MQ=255)
gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGa > 1:9635207/1‑76 (MQ=255)
gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGa > 1:2693295/1‑76 (MQ=255)
gTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGa > 2:2006429/1‑76 (MQ=255)
ttAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAc < 2:2927286/76‑1 (MQ=255)
ttAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGAc > 2:4654184/1‑76 (MQ=255)
tAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCACCGAcg > 2:6931260/1‑76 (MQ=255)
aaCCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgc < 1:5459915/76‑1 (MQ=255)
aaCCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCCGAcgc > 2:3819790/1‑76 (MQ=255)
ccGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgc > 2:9497604/1‑76 (MQ=255)
cGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgcg < 2:418639/76‑1 (MQ=255)
gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGt > 1:8142064/1‑76 (MQ=255)
gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCCCGCAGACGCGCGt > 1:1626547/1‑76 (MQ=255)
tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGAcgcgcggt > 2:9146764/1‑74 (MQ=255)
tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt < 2:9291021/76‑1 (MQ=255)
tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt < 1:4098328/76‑1 (MQ=255)
tGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGtt < 2:64076/76‑1 (MQ=255)
gagaTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtct < 2:5878630/76‑1 (MQ=255)
agaTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTtctc < 1:9068370/76‑1 (MQ=255)
gaTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTTTcc > 2:7400990/1‑76 (MQ=255)
aTAACCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCt < 2:7784810/76‑1 (MQ=255)
aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCCGACCCGCGCTCTCCCGt > 1:1053010/1‑76 (MQ=255)
aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGt > 1:7583547/1‑76 (MQ=255)
aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGt < 2:9032823/76‑1 (MQ=255)
aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCCGt > 1:136792/1‑76 (MQ=255)
aaCCTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCTGt > 2:3932110/1‑76 (MQ=255)
ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCCGCCGCGCGTTCTCCCGttt > 1:8613912/1‑76 (MQ=255)
ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGttt < 2:151151/76‑1 (MQ=255)
ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGttt < 2:6754423/76‑1 (MQ=255)
ccTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGttt < 2:8794379/76‑1 (MQ=255)
cTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTg < 1:5613359/76‑1 (MQ=255)
cTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTg < 1:501409/76‑1 (MQ=255)
cTGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCCGtttt > 2:9385872/1‑75 (MQ=255)
tGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGt < 1:4392844/76‑1 (MQ=255)
tGAGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGt < 2:1434400/76‑1 (MQ=255)
aGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAt < 2:8192461/76‑1 (MQ=255)
aGTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCTGTTTGTAt > 2:7066415/1‑76 (MQ=255)
gTCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAtt < 2:790234/76‑1 (MQ=255)
tCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTAttt > 1:7914675/1‑75 (MQ=255)
tCACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTg < 1:59511/76‑1 (MQ=255)
cACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGt > 1:6419855/1‑76 (MQ=255)
cACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGt < 1:3525460/76‑1 (MQ=255)
cACCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGt < 2:3647111/76‑1 (MQ=255)
aCCATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTc < 2:9748353/76‑1 (MQ=255)
ccATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTcc < 1:9361609/76‑1 (MQ=255)
cATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCa > 1:7149800/1‑76 (MQ=255)
cATTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCa < 1:4008392/76‑1 (MQ=255)
aTTTTCTGCTCATCCTGACTCAGCGGGGAGCCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCaa < 1:3643395/76‑1 (MQ=255)
aTTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCCGACGCGCGTTCTCCTGTTTGTATTGTCCaa > 1:9907295/1‑76 (MQ=255)
aTTTTCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCaa > 2:10142365/1‑76 (MQ=255)
ttttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCCCGTTCTCCTGTTTGTATTGTCCCAg > 2:1715848/1‑76 (MQ=255)
tttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc < 1:2037624/76‑1 (MQ=255)
tttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc < 1:8290423/76‑1 (MQ=255)
tttCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGc > 1:679905/1‑76 (MQ=255)
tCTGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCAt < 2:303168/76‑1 (MQ=255)
cggCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATc < 1:1148263/74‑1 (MQ=255)
tGCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATca < 2:9948403/76‑1 (MQ=255)
gCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag < 2:1358700/76‑1 (MQ=255)
gCTCATCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag < 1:8440926/76‑1 (MQ=255)
aTCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag < 1:8660155/72‑1 (MQ=255)
tCCTGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGtt < 1:375605/76‑1 (MQ=255)
tGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCAc < 1:2438179/76‑1 (MQ=255)
tGACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGGTCTCCCGTTTTTATTGTCCCAGCATCAGCAGTTTAc > 2:2702983/1‑76 (MQ=255)
gACTCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACt < 2:560518/76‑1 (MQ=255)
gACTCAGCGGGGAACCCAGCAGCTCCCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATAAGCAGTTCACt > 2:7600241/1‑76 (MQ=255)
|
ATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACT > REL606/3327460‑3327605
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
