Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A127 F1 I1 R1
|
15 |
330.5 |
20417406 |
95.5% |
19498622 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,650,140 |
A→G |
R69G (AGA→GGA) |
yiaG → |
predicted transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,650,140 | 0 | A | G | 97.6%
| 1245.0
/ 9.6
| 289 | R69G (AGA→GGA) | yiaG | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (135/147); total (138/151) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 290 total aligned reads displayed.
GACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGC > REL606/3650067‑3650212
|
gACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGa < 2:7326324/76‑1 (MQ=255)
gACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGa > 2:1106931/1‑76 (MQ=255)
aCAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAc < 1:4055973/76‑1 (MQ=255)
aCAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAc < 1:503799/76‑1 (MQ=255)
cAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAcg < 2:4575380/76‑1 (MQ=255)
cAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGAcg < 1:6455175/76‑1 (MQ=255)
aGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAcgc > 1:4664805/1‑76 (MQ=255)
aGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGAcgc > 2:5479915/1‑76 (MQ=255)
gATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGt < 1:8863595/76‑1 (MQ=255)
aTTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTg < 1:1421472/76‑1 (MQ=255)
aTTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTg > 2:5588844/1‑76 (MQ=255)
ttAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGa < 1:99464/76‑1 (MQ=255)
ttAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGa < 2:7614681/76‑1 (MQ=255)
ttAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGa < 1:2093424/76‑1 (MQ=255)
tAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGaa < 1:3401441/76‑1 (MQ=255)
aaaaaTCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAg < 1:5454831/76‑1 (MQ=255)
aaaaaTCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAg > 1:9465872/1‑76 (MQ=255)
aaTCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGCAAAGGAATGGGAATCCGGACGCGTGAAGCCt > 1:8264915/1‑76 (MQ=255)
cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa < 1:4368611/76‑1 (MQ=255)
cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa < 1:2489646/76‑1 (MQ=255)
cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa > 1:476619/1‑76 (MQ=255)
cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa > 1:1986065/1‑76 (MQ=255)
cGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCa < 1:1232935/76‑1 (MQ=255)
gatgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCaa < 1:1561379/76‑1 (MQ=255)
atgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAg < 2:1745440/76‑1 (MQ=255)
atgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAg < 1:1808024/76‑1 (MQ=255)
tgatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGt < 1:4825819/76‑1 (MQ=255)
gatTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTg > 1:5906089/1‑76 (MQ=255)
tttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGcc > 2:1836802/1‑76 (MQ=255)
tttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGcc < 2:3347169/76‑1 (MQ=255)
tttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGcc < 2:6313524/76‑1 (MQ=255)
ttCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCg < 1:6208582/76‑1 (MQ=255)
cGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGaa < 2:8950826/76‑1 (MQ=255)
cGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGaa < 2:5787373/76‑1 (MQ=255)
cccGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACt < 1:5789181/76‑1 (MQ=255)
cccGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACt > 2:8397885/1‑76 (MQ=255)
ccGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTa < 1:8115906/76‑1 (MQ=255)
cGGGTTTTGGGCGTGTCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:1224963/1‑76 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:6981407/1‑76 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:7423793/1‑76 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa < 2:3438030/76‑1 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:4391789/1‑76 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:4134816/1‑76 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa > 2:9669366/1‑76 (MQ=255)
cGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaa < 1:7317028/76‑1 (MQ=255)
gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa > 1:1029699/1‑76 (MQ=255)
gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa > 2:2920513/1‑76 (MQ=255)
gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa > 2:1537141/1‑76 (MQ=255)
gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa < 1:5289710/76‑1 (MQ=255)
gggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaa < 1:9669366/76‑1 (MQ=255)
ggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTaaaa < 1:9615996/76‑1 (MQ=255)
tttGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTg > 1:8415832/1‑76 (MQ=255)
tGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGAt > 1:1013082/1‑76 (MQ=255)
gggCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATg > 2:6065062/1‑76 (MQ=255)
ggCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGc < 1:4447619/76‑1 (MQ=255)
gTATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGtt < 1:2397530/76‑1 (MQ=255)
tATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGttt < 2:449817/76‑1 (MQ=255)
tATCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGttt > 2:9266523/1‑76 (MQ=255)
aTCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTg < 2:8818885/76‑1 (MQ=255)
tCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGa < 1:2551681/76‑1 (MQ=255)
tCAGTCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGa < 1:7501882/76‑1 (MQ=255)
tCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCa < 1:7983645/76‑1 (MQ=255)
tCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCa < 2:8251825/76‑1 (MQ=255)
tCGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCa < 2:8626826/76‑1 (MQ=255)
cGCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCaa > 2:6178292/1‑76 (MQ=255)
gCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAg < 1:3197321/76‑1 (MQ=255)
gCCATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAg < 1:6998412/76‑1 (MQ=255)
ccATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGc > 1:6063805/1‑76 (MQ=255)
ccATGGTAAAGGAATGGGAATCCGGACGCGTAAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGc > 2:7879692/1‑76 (MQ=255)
cATGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGcc > 1:5619679/1‑76 (MQ=255)
cATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGcc > 2:9853064/1‑76 (MQ=255)
aTGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCa < 2:1296076/76‑1 (MQ=255)
tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa > 1:5129242/1‑76 (MQ=255)
tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa < 2:3943047/76‑1 (MQ=255)
tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa > 1:3009784/1‑76 (MQ=255)
tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa < 2:6622075/76‑1 (MQ=255)
tGGTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCaa > 2:1170467/1‑76 (MQ=255)
ggTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAc > 1:667551/1‑76 (MQ=255)
ggTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAc < 1:1727032/76‑1 (MQ=255)
gTAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAcc > 2:8910604/1‑76 (MQ=255)
tAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAccc < 2:2300839/76‑1 (MQ=255)
tAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAccc < 2:2850963/76‑1 (MQ=255)
tAAAGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAccc > 1:8650832/1‑76 (MQ=255)
aaaGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCg < 2:5059147/76‑1 (MQ=255)
aaaGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCg > 2:3589597/1‑76 (MQ=255)
aaGGAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCgg < 2:6496736/76‑1 (MQ=255)
gAATGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAt < 2:4928674/76‑1 (MQ=255)
aaTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAtt < 1:10039599/76‑1 (MQ=255)
aaTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAtt < 2:5268137/76‑1 (MQ=255)
aTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATta < 1:3766159/76‑1 (MQ=255)
aTGGGAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATta > 1:10015869/1‑76 (MQ=255)
gggAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaag < 1:790075/76‑1 (MQ=255)
gggAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaag > 2:2468115/1‑76 (MQ=255)
ggAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagt < 1:2148869/76‑1 (MQ=255)
gAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagta > 1:9570973/1‑76 (MQ=255)
gAATCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagta < 2:3234031/76‑1 (MQ=255)
aaTCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagtaa < 2:7149070/76‑1 (MQ=255)
aTCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATtaagtaag > 2:2292151/1‑76 (MQ=255)
tCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGc < 1:9148005/76‑1 (MQ=255)
tCCGGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGc < 1:2730900/76‑1 (MQ=255)
|
GACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGC > REL606/3650067‑3650212
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
