Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A127 F1 I1 R1
|
15 |
330.5 |
20417406 |
95.5% |
19498622 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,168,348 |
G→T |
G1130C (GGT→TGT) |
rpoC → |
DNA‑directed RNA polymerase subunit beta' |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,168,348 | 0 | G | T | 99.3%
| 1221.9
/ 1.0
| 296 | G1130C (GGT→TGT) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (149/145); total (151/145) |
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 |
Only 100 of 311 total aligned reads displayed.
GATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGG > REL606/4168275‑4168420
|
gATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGt > 1:3159039/1‑76 (MQ=255)
aTTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGTAGGAATCCGGCTGTa > 1:6482335/1‑76 (MQ=255)
aTTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTa > 2:6968191/1‑76 (MQ=255)
tGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTAcc > 1:5062399/1‑76 (MQ=255)
gTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCa < 1:1263587/76‑1 (MQ=255)
tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg > 2:119329/1‑76 (MQ=255)
tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg < 2:7686417/76‑1 (MQ=255)
tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg < 2:1724105/76‑1 (MQ=255)
tCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAg < 2:252831/76‑1 (MQ=255)
cAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAgg < 1:3781096/76‑1 (MQ=255)
aGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGa < 2:3350127/76‑1 (MQ=255)
cTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACa > 2:5497629/1‑76 (MQ=255)
tGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACAt > 2:2351504/1‑76 (MQ=255)
ggAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATc > 1:823075/1‑76 (MQ=255)
gAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCa < 2:1085022/76‑1 (MQ=255)
gAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCa > 2:9066250/1‑76 (MQ=255)
gaGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCAc < 1:3916662/75‑1 (MQ=255)
aaGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCAc > 2:7401634/1‑76 (MQ=255)
aGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCAcc > 2:2688860/1‑76 (MQ=255)
gATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCg > 2:3912882/1‑76 (MQ=255)
gATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCg > 2:5065119/1‑76 (MQ=255)
aTGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCgg < 1:1652277/76‑1 (MQ=255)
tGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggt > 2:1418446/1‑76 (MQ=255)
ggCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggtg > 1:2954315/1‑76 (MQ=255)
cGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggtggt > 1:9090696/1‑76 (MQ=255)
cGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCggtggt > 1:9706054/1‑76 (MQ=255)
gTACAGATCAGCTCTGGTGACTCCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTc < 2:5897743/76‑1 (MQ=39)
gTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTc > 2:7191403/1‑76 (MQ=255)
tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt > 1:8587102/1‑76 (MQ=255)
tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt < 1:7997424/76‑1 (MQ=255)
tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt < 2:5699794/76‑1 (MQ=255)
tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCt > 2:6305878/1‑76 (MQ=255)
aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg > 2:2225631/1‑76 (MQ=255)
aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg < 2:1903019/76‑1 (MQ=255)
aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg < 2:2892711/76‑1 (MQ=255)
aCAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTg > 2:3264411/1‑76 (MQ=255)
cAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGc > 1:8767565/1‑76 (MQ=255)
aGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGcc < 1:1410677/76‑1 (MQ=255)
gATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcg > 1:4462001/1‑76 (MQ=255)
aTCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgc > 2:4672571/1‑76 (MQ=255)
tCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcg > 2:2821744/1‑76 (MQ=255)
cAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcgc > 2:4599724/1‑76 (MQ=255)
cAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcgc > 1:1076188/1‑76 (MQ=255)
aGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCcgcgcg > 1:901569/1‑76 (MQ=255)
ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTTTGCGGCGCGtt > 1:7825699/1‑76 (MQ=255)
ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 1:4801136/76‑1 (MQ=255)
ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 1:9919616/76‑1 (MQ=255)
ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 2:4928177/76‑1 (MQ=255)
ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGc > 1:9147423/1‑76 (MQ=255)
ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGc > 1:8664119/1‑76 (MQ=255)
ctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGc > 1:6075749/1‑76 (MQ=255)
ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCgg < 2:4266817/76‑1 (MQ=255)
ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCgg > 1:4912198/1‑76 (MQ=255)
ggTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCgg < 1:2431840/76‑1 (MQ=255)
gACACCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCGAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGAcc < 1:2777999/76‑1 (MQ=255)
acacCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCt < 1:4544966/76‑1 (MQ=255)
acacCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCt < 2:10179893/76‑1 (MQ=255)
cacCCTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTg < 2:6137336/76‑1 (MQ=255)
cccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGtt < 1:1973663/76‑1 (MQ=255)
ccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTc > 1:6571931/1‑76 (MQ=255)
ccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTc < 1:10055581/76‑1 (MQ=255)
ccTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTc < 2:2067284/76‑1 (MQ=255)
cTGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCg < 1:5160240/76‑1 (MQ=255)
tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa < 1:6340200/76‑1 (MQ=255)
tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa > 2:3661203/1‑76 (MQ=255)
tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa < 1:7285816/76‑1 (MQ=255)
tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa > 2:9440005/1‑76 (MQ=255)
tGGCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGa < 1:9856774/76‑1 (MQ=255)
ggCGCGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGaa > 2:9169717/1‑76 (MQ=255)
gcgcGTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAg < 2:743907/76‑1 (MQ=255)
cgcgTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc > 2:9592052/1‑76 (MQ=255)
cgcgTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc < 1:9556958/76‑1 (MQ=255)
cgTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAc > 2:4843185/1‑76 (MQ=255)
gTATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcg < 2:6755029/76‑1 (MQ=255)
tATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt < 1:764006/76‑1 (MQ=255)
tATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgt > 2:3190011/1‑76 (MQ=255)
tATTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACATGTTCGAAGCAcgt > 1:6197251/1‑76 (MQ=255)
aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc < 2:8879711/76‑1 (MQ=255)
aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc < 2:821741/76‑1 (MQ=255)
aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc < 2:4107686/76‑1 (MQ=255)
aTTCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtc > 1:5972731/1‑76 (MQ=255)
ttCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtcg < 2:4408222/76‑1 (MQ=255)
ttCCGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCAcgtcg < 2:9743770/76‑1 (MQ=255)
ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc > 2:3941260/1‑76 (MQ=255)
ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc < 2:8154220/76‑1 (MQ=255)
ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc < 1:3370585/76‑1 (MQ=255)
ccGCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACgtcgtc < 2:8966934/76‑1 (MQ=255)
gCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCg < 1:2359410/76‑1 (MQ=255)
gCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCg < 1:8323838/76‑1 (MQ=255)
gCAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCg < 1:3588046/76‑1 (MQ=255)
cAGGAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGa > 2:8796904/1‑76 (MQ=255)
ggAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaaa < 2:9855174/76‑1 (MQ=255)
ggAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaaa > 1:4000162/1‑76 (MQ=255)
ggAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGaaa > 1:6831986/1‑76 (MQ=255)
gAATCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAag < 2:5230599/76‑1 (MQ=255)
aTCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAagag < 1:5633421/76‑1 (MQ=255)
tCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGc > 2:8528302/1‑76 (MQ=255)
tCCGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGc > 1:2137267/1‑76 (MQ=255)
ccGGCTGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGcc > 1:1750699/1‑76 (MQ=255)
ggctgTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCgg > 2:5809925/1‑76 (MQ=255)
|
GATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGG > REL606/4168275‑4168420
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
