Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A74 F1 I1 R1
|
16 |
49.9 |
3507628 |
96.2% |
3374338 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,499,711 |
A→C |
S365A (TCA→GCA) |
glgC ← |
glucose‑1‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,499,711 | 0 | A | C | 100.0%
| 130.2
/ NA
| 32 | S365A (TCA→GCA) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (15/17); total (15/17) |
CAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAAC > REL606/3499638‑3499783
|
cAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgcat < 1:139611/76‑1 (MQ=255)
gcgcAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCAcg < 2:497349/76‑1 (MQ=255)
gcgcAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCAcg < 1:794464/76‑1 (MQ=255)
cgcAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCAcgc > 1:748502/1‑76 (MQ=255)
gcAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCAcgcg < 1:1055792/76‑1 (MQ=255)
cggACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGa > 1:1307994/3‑76 (MQ=255)
aGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGaa > 1:1336208/1‑76 (MQ=255)
gACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAAc > 1:1461808/1‑76 (MQ=255)
cACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAAcgcgcg > 2:1617341/1‑76 (MQ=255)
cACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAAcgcgcg > 2:1272530/1‑76 (MQ=255)
cGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCgag < 1:1345340/76‑1 (MQ=255)
cGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCgag < 2:1456114/76‑1 (MQ=255)
gCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGagaac < 1:1570331/76‑1 (MQ=255)
cGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGagaaca < 1:344218/76‑1 (MQ=255)
gACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGagaacag > 2:970817/1‑76 (MQ=255)
gACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGagaacag < 2:941577/76‑1 (MQ=255)
aCCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGagaacaga < 1:1548344/76‑1 (MQ=255)
cTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGagaacagaac < 1:679506/76‑1 (MQ=255)
aCCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACgg > 2:581319/1‑76 (MQ=255)
cccATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGa < 2:1212483/76‑1 (MQ=255)
caCTTCCGGTAACAATACGGCGGAATCAATGTTGAAGAATGCATTCACGAGATCGCGCGAGAACAGCACGGACTGc > 2:416656/2‑76 (MQ=255)
aCTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGca < 2:884874/76‑1 (MQ=255)
tCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGcacca > 1:1593598/1‑76 (MQ=255)
tCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGcacca < 1:1588904/76‑1 (MQ=255)
aaTACGGCGGAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAc < 1:413617/76‑1 (MQ=255)
cggcggAATCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGga < 1:272771/76‑1 (MQ=255)
aaTCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcac < 2:132723/76‑1 (MQ=255)
aTCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcaca > 2:946962/1‑76 (MQ=255)
tCAATGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacac < 2:770918/76‑1 (MQ=255)
aaTGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACaa > 1:665029/1‑76 (MQ=255)
tGTTGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAcc > 1:814188/1‑76 (MQ=255)
ttGCAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgc > 2:370580/1‑76 (MQ=255)
cAGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCgg > 2:1587713/1‑76 (MQ=255)
aGAATGCATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGa > 1:1560724/1‑76 (MQ=255)
aatgcatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACTGGAGATCACACAACCGCCGGaaa > 2:1361308/1‑76 (MQ=255)
aatgcatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaaa > 2:1102054/1‑76 (MQ=255)
atgcatTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAAc < 1:1184695/76‑1 (MQ=255)
|
CAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAAC > REL606/3499638‑3499783
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
