breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
JC | 474,296 | Δ11 bp | coding (1000‑1010/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 649,489 | C→A | R438L (CGC→CTC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 1,290,813 | C→T | Q260* (CAG→TAG) | hnr → | response regulator of RpoS |
MC JC | 1,304,524 | Δ367 bp | [yciU]–[cls] | [yciU], [cls] | |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
RA | 1,444,490 | A→C | R5R (AGG→CGG) | ydbD → | hypothetical protein |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,145,965 | T→C | D34D (GAT→GAC) | rpoD → | RNA polymerase sigma factor |
RA | 3,477,922 | A→C | V250G (GTC→GGC) | malQ ← | 4‑alpha‑glucanotransferase (amylomaltase) |
RA | 3,499,711 | A→C | S365A (TCA→GCA) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
RA | 4,167,458 | A→G | E833G (GAG→GGG) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
RA | 4,462,990 | T→A | F76L (TTT→TTA) | yjgN → | conserved inner membrane protein |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 3893551 | 3901930 | 8380 | 52 [7] | [0] 58 | insJ‑5–[yieO] | insJ‑5,insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 3652533 | NA (NA) | 54 (1.020) | 46/146 | 0.1 | 100% | noncoding (1443/1443 nt) | IS150 | repeat region |
? | REL606 | 3901931 = | 0 (0.000) | coding (489/1428 nt) | yieO | predicted multidrug or homocysteine efflux system | |||||
* | ? | REL606 | = 3893550 | 5 (0.090) | 43 (0.820) +GGG |
36/142 | 0.4 | 89.8% | intergenic (+5/‑51) | kup/insJ‑5 | potassium transporter/IS150 hypothetical protein |
? | REL606 | 3893551 = | NA (NA) | intergenic (+6/‑50) | kup/insJ‑5 | potassium transporter/IS150 hypothetical protein |