breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC 474,296 Δ11 bp coding (1000‑1010/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 649,489 C→A R438L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 1,290,813 C→T Q260* (CAG→TAG)  hnr → response regulator of RpoS
MC JC 1,304,524 Δ367 bp [yciU][cls] [yciU], [cls]
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,444,490 A→C R5R (AGG→CGG)  ydbD → hypothetical protein
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,145,965 T→C D34D (GAT→GAC rpoD → RNA polymerase sigma factor
RA 3,477,922 A→C V250G (GTC→GGC)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,499,711 A→C S365A (TCA→GCA)  glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
RA 4,167,458 A→G E833G (GAG→GGG)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA 4,462,990 T→A F76L (TTT→TTA yjgN → conserved inner membrane protein
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3893551 3901930 8380 52 [7] [0] 58 insJ‑5–[yieO] insJ‑5,insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3652533NA (NA)54 (1.020) 46/146 0.1 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901931 = 0 (0.000)coding (489/1428 nt) yieO predicted multidrug or homocysteine efflux system
* ? REL606 = 38935505 (0.090)43 (0.820)
+GGG
36/142 0.4 89.8% intergenic (+5/‑51) kup/insJ‑5 potassium transporter/IS150 hypothetical protein
?REL606 3893551 = NA (NA)intergenic (+6/‑50) kup/insJ‑5 potassium transporter/IS150 hypothetical protein