breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL60635,4270TG64.7% 3.7 / 16.8 17G180G (GGT→GGGcarBcarbamoyl‑phosphate synthase large subunit
*REL6063,401,0240T.43.9% 183.9 / 156.6 82coding (396/2115 nt)fusAelongation factor EF‑2
*REL6064,254,7410TG43.6% 7.1 / 255.8 134pseudogene (338/936 nt)yjcChypothetical protein; b4061_1
*REL6064,393,0610TG40.0% 111.8 / 33.2 70V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6062,296,3290AC37.2% 71.8 / 24.9 43intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6063,492,0820TG35.0% 107.3 / 20.6 60V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6061,747,9030GT33.3% 155.8 / 79.6 84A8S (GCA→TCA) ydiLhypothetical protein
*REL6063,733,7190CG31.2% 61.8 / 13.0 32G63G (GGC→GGGgumDADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
*REL6063,235,4520TG30.6% 46.7 / 21.0 37F85V (TTT→GTT) yhbQhypothetical protein
*REL606254,1010TG28.6% 21.7 / 11.1 21pseudogene (530/1710 nt)fhiAb0229; flagellar system protein
*REL6064,195,8740AC28.4% 153.8 / 12.5 67T318P (ACC→CCC) aceBmalate synthase

Marginal new junction evidence (lowest skew 10 of 59 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 203729540 (0.730)8 (0.160) 7/134 4.9 19.1% coding (836/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2037489 32 (0.660)coding (642/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 2041277 =34 (0.620)7 (0.140) 7/136 5.0 13.9% coding (107/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041470 = 56 (1.130)coding (1351/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 203691756 (1.030)9 (0.180) 7/140 5.1 18.3% coding (42/1113 nt) wbbC putative glycosyltransferase WbbC
?REL606 = 2037192 28 (0.550)coding (939/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 2041220 =38 (0.700)7 (0.140) 7/142 5.2 12.8% coding (164/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041465 = 59 (1.140)coding (1356/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2039283 =29 (0.530)10 (0.190) 7/142 5.2 23.1% coding (412/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039547 = 39 (0.760)coding (148/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 203883330 (0.550)7 (0.140) 6/134 5.3 21.6% coding (116/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 = 2039157 24 (0.490)coding (538/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 21891 =46 (0.840)8 (0.160) 6/134 5.3 13.4% intergenic (‑116/+317) ECB_00021/ECB_00022 putative usher protein/conserved hypothetical protein
?REL606 22087 = 62 (1.270)intergenic (‑312/+121) ECB_00021/ECB_00022 putative usher protein/conserved hypothetical protein
* ? REL606 4487211 =115 (2.110)7 (0.140) 6/140 5.5 6.2% coding (33/1119 nt) yjhC predicted oxidoreductase
?REL606 4487425 = 103 (2.030)coding (247/1119 nt) yjhC predicted oxidoreductase
* ? REL606 2039335 =28 (0.510)7 (0.140) 6/138 5.5 19.0% coding (360/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039563 = 34 (0.680)coding (132/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 4486353 =102 (1.870)6 (0.130) 5/130 5.7 6.1% coding (404/1218 nt) yjhB predicted transporter
?REL606 4486544 = 97 (2.060)coding (595/1218 nt) yjhB predicted transporter