Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A106 F1 I1 R1
|
16 |
286.4 |
19211214 |
90.7% |
17424571 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,886,608 |
G→A |
intergenic (‑438/+231) |
yobF ← / ← yebO |
hypothetical protein/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,886,608 | 0 | G | A | 97.3%
| 1318.3
/ 13.5
| 336 | intergenic (‑438/+231) | yobF/yebO | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base A (138/189); total (142/194) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
Only 100 of 339 total aligned reads displayed.
AGTCAATCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACGTTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGAAAAT > REL606/1886535‑1886680
|
gTCAATCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACAttt > 1:5467099/1‑76 (MQ=255)
cAATCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCg > 1:4022304/1‑76 (MQ=255)
cAATCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCg > 1:4227758/1‑76 (MQ=255)
cAATCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCg > 2:6644107/1‑76 (MQ=255)
aTCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCt < 1:34576/76‑1 (MQ=255)
aTCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCt > 2:3198591/1‑76 (MQ=255)
aTCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTCCGCt < 1:8198490/76‑1 (MQ=255)
tCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACGTTTCGCta > 2:8396638/1‑76 (MQ=255)
ccccAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCtaa > 1:4436334/1‑76 (MQ=255)
ccccAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCtaa > 1:1848615/1‑76 (MQ=255)
ccccAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCtaa < 2:8036400/76‑1 (MQ=255)
cccAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCtaat > 2:3345682/1‑76 (MQ=255)
aaaGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTaataaa < 2:4451156/76‑1 (MQ=255)
gggATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAataaataa > 1:3673377/1‑76 (MQ=255)
aTTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATAc < 2:2444402/76‑1 (MQ=255)
ttGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATAcc > 1:5040020/1‑76 (MQ=255)
ttGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATAcc < 1:7328740/76‑1 (MQ=255)
tGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCa < 1:8044531/76‑1 (MQ=255)
tGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCa < 2:5011090/76‑1 (MQ=255)
tGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCa > 1:4137427/1‑76 (MQ=255)
aaaaTTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACg < 2:9232322/76‑1 (MQ=255)
aaaaTAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATc < 1:5926699/76‑1 (MQ=255)
aaaaTAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATc > 1:5857720/1‑76 (MQ=255)
aaaaTAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATc > 1:7237515/1‑76 (MQ=255)
aaGAAAAATTGATGAATGAGCAAAAAATCAATAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACaaa > 1:1273685/1‑76 (MQ=255)
aGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAAt > 1:3291255/1‑76 (MQ=255)
aaaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATcc < 1:9387472/76‑1 (MQ=255)
aaaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATcc < 1:895827/76‑1 (MQ=255)
aaaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATcc < 1:6557958/76‑1 (MQ=255)
aaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCg > 2:6439453/1‑76 (MQ=255)
aaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCg > 2:765343/1‑76 (MQ=255)
aaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCg > 2:1236415/1‑76 (MQ=255)
aaaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCg > 1:8417383/1‑76 (MQ=255)
aaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGa > 2:5627987/1‑76 (MQ=255)
aaaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGa > 1:2207229/1‑76 (MQ=255)
aaTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGAt > 2:1994450/1‑76 (MQ=255)
aTTGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATg < 1:1923212/76‑1 (MQ=255)
ttGATGAATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGt < 1:3861199/76‑1 (MQ=255)
tgatgaATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTa > 1:1248751/1‑76 (MQ=255)
gatgaATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTaa < 2:2198243/76‑1 (MQ=255)
gatgaATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTaa > 1:7682923/1‑76 (MQ=255)
gatgaATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTaa < 2:2645490/76‑1 (MQ=255)
gatgaATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTaa < 1:3170240/76‑1 (MQ=255)
gatgaATGAGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTaa > 1:4889547/1‑76 (MQ=255)
atgaatgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTaaa < 2:8626231/76‑1 (MQ=255)
tgaatgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAAc < 2:8229416/76‑1 (MQ=255)
tgaatgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAAc < 1:2540333/76‑1 (MQ=255)
gaatgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACa < 2:3463077/76‑1 (MQ=255)
gaatgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACa < 2:1073181/76‑1 (MQ=255)
aatgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACAt > 1:2319304/1‑76 (MQ=255)
atgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATc > 1:2355647/1‑76 (MQ=255)
tgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATcc > 2:737487/1‑76 (MQ=255)
tgaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATcc > 2:1492042/1‑76 (MQ=255)
gaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCt < 1:1412450/76‑1 (MQ=255)
gaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCt > 2:6052134/1‑76 (MQ=255)
gaGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCt < 2:1828640/76‑1 (MQ=255)
aGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCta > 2:2934939/1‑76 (MQ=255)
aGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCta > 2:9419016/1‑76 (MQ=255)
aGCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCta < 1:5636340/76‑1 (MQ=255)
gCAAAAAATCAAGAGAGAAACGTTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaa < 1:7575658/76‑1 (MQ=255)
gCAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaa < 2:1775850/76‑1 (MQ=255)
cAAAAAATCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaat < 1:5244266/76‑1 (MQ=255)
aaaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaata < 1:7088485/76‑1 (MQ=255)
aaaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaata > 2:31560/1‑76 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:6824305/76‑1 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:1580328/76‑1 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa > 2:6927453/1‑76 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:2116043/76‑1 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:6124607/76‑1 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:466205/76‑1 (MQ=255)
aaaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:5320357/76‑1 (MQ=255)
aaaaaCCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCtaataa < 1:698793/76‑1 (MQ=255)
aaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATaaa > 1:9102335/1‑76 (MQ=255)
aaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATaaa < 2:8512875/76‑1 (MQ=255)
aaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATaaa > 1:7276886/1‑76 (MQ=255)
aaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATaaa > 1:5067677/1‑76 (MQ=255)
aaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATaaa < 2:6312359/76‑1 (MQ=255)
aaaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATaaa < 2:5462450/76‑1 (MQ=255)
aaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAAt < 1:6420954/76‑1 (MQ=255)
aaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAAt > 2:3690412/1‑76 (MQ=255)
aaaTCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAAt > 1:6769620/1‑76 (MQ=255)
aTCAAGAGAGAAACGTTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTa < 1:876498/76‑1 (MQ=255)
tCAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTaa > 2:4231406/1‑76 (MQ=255)
cAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAAt < 1:6288323/76‑1 (MQ=255)
cAAGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAAt < 1:204599/76‑1 (MQ=255)
aaGAGAGAAACATTTCTCTAATAAATAATACCACGCTTAATCACAAATCCGATGGAAACATCCTAATAAATTAATg < 2:4436333/76‑1 (MQ=255)
aaGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATg > 2:4844793/1‑76 (MQ=255)
aaGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATg < 1:785615/76‑1 (MQ=255)
aaGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATg < 1:1033015/76‑1 (MQ=255)
aaGAGAGAAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATg > 2:7216700/1‑76 (MQ=255)
gagagaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAg < 1:8009029/76‑1 (MQ=255)
gagaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGa < 1:2918129/76‑1 (MQ=255)
gagaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGa < 1:9499821/76‑1 (MQ=255)
agaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGaa < 1:2974188/76‑1 (MQ=255)
gaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGaaa < 2:52719/76‑1 (MQ=255)
gaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGaaa > 1:957446/1‑76 (MQ=255)
gaAACATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGaaa > 1:5733984/1‑76 (MQ=255)
aaaCATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGaaaa < 1:2592664/76‑1 (MQ=255)
aaCGTTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGAAAat < 1:1695097/76‑1 (MQ=255)
aaCATTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGAAAat > 1:4380767/1‑76 (MQ=255)
|
AGTCAATCCCCAAAGGGATTGAAAATTTAAAATAAGAAAAATTGATGAATGAGCAAAAAATCAAGAGAGAAACGTTTCGCTAATAAATAATACCACGCTTAATCACAAATCCGATGTAAACATCCTAATAAATTAATGAGGAAAAT > REL606/1886535‑1886680
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
