Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A106 F1 I1 R1
|
16 |
286.4 |
19211214 |
90.7% |
17424571 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,593,944 |
T→A |
I166N (ATC→AAC) |
bglJ → |
DNA‑binding transcriptional activator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,593,944 | 0 | T | A | 99.1%
| 1319.8
/ 0.7
| 331 | I166N (ATC→AAC) | bglJ | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (125/203); total (127/204) |
| Fisher's exact test for biased strand distribution p-value = 5.61e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01 |
Only 100 of 332 total aligned reads displayed.
ATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGA > REL606/4593871‑4594016
|
atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCTGAGCAGCTTAAACGCAATATCAAAACGAAcc < 2:9219534/76‑1 (MQ=255)
atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAAcc < 2:1150030/76‑1 (MQ=255)
atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAAcc < 2:669880/76‑1 (MQ=255)
tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCg < 1:821825/76‑1 (MQ=255)
tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCg > 1:5703544/1‑76 (MQ=255)
tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCg < 1:3254307/76‑1 (MQ=255)
agatGCGATGTATGGCGCGTGGCTACTCAATGACACAAATGGGCGAGCAGCTTAAACGCAATATCAAAACGAACCg < 1:645240/73‑1 (MQ=255)
cTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACAt < 2:4356327/76‑1 (MQ=255)
cTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACAt < 2:6379741/76‑1 (MQ=255)
ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATaa < 1:1439291/76‑1 (MQ=255)
ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATaa > 2:4411099/1‑76 (MQ=255)
ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATaa > 1:7508944/1‑76 (MQ=255)
tATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATaaa > 2:5770956/1‑76 (MQ=255)
tGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAAtt < 1:4655226/76‑1 (MQ=255)
tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTa > 2:7586098/1‑76 (MQ=255)
tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTa < 2:7213677/76‑1 (MQ=255)
tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTa > 2:7080972/1‑76 (MQ=255)
tGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAAtgtg < 1:9542672/76‑1 (MQ=255)
tGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAAtgtg < 1:7950226/76‑1 (MQ=255)
gCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGAt > 1:1200388/1‑76 (MQ=255)
cTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATg > 2:7503255/1‑76 (MQ=255)
cTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATg > 1:1746600/1‑76 (MQ=255)
tACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGt < 2:2806073/76‑1 (MQ=255)
cTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCg < 1:799853/76‑1 (MQ=255)
cTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCg < 1:7047463/76‑1 (MQ=255)
cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa > 1:2795163/1‑76 (MQ=255)
cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa > 1:5374506/1‑76 (MQ=255)
cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa < 2:3333294/76‑1 (MQ=255)
cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa > 1:3458720/1‑76 (MQ=255)
aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaaa < 2:5974131/76‑1 (MQ=255)
tGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACt > 1:5551687/1‑76 (MQ=255)
gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTg < 1:7724954/76‑1 (MQ=255)
gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTg < 1:2491936/76‑1 (MQ=255)
gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTg > 1:8257846/1‑76 (MQ=255)
aaaTTGCAGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGt < 2:4273593/76‑1 (MQ=255)
aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTc > 2:4806852/1‑76 (MQ=255)
aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTc < 2:7898865/76‑1 (MQ=255)
ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg < 2:1746600/76‑1 (MQ=255)
ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg < 2:6554447/76‑1 (MQ=255)
ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg > 1:3586476/1‑76 (MQ=255)
ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg < 2:8305007/76‑1 (MQ=255)
tGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGt > 1:1521204/1‑76 (MQ=255)
gCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt < 2:2463953/76‑1 (MQ=255)
cGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt > 2:9447195/1‑76 (MQ=255)
cGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt > 1:653981/1‑76 (MQ=255)
gAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg < 2:5670342/76‑1 (MQ=255)
agcagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa < 2:5551687/76‑1 (MQ=255)
agcagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa < 2:1908296/76‑1 (MQ=255)
cagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg > 2:4949110/1‑76 (MQ=255)
cagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg > 2:31861/1‑76 (MQ=255)
cagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg > 1:2200326/1‑76 (MQ=255)
agcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc > 2:2334/1‑76 (MQ=255)
agcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc > 2:4622935/1‑76 (MQ=255)
agcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc > 2:2644454/1‑76 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:2887848/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:3544233/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:3659341/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:421483/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:8398168/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 1:6753474/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:1970110/76‑1 (MQ=255)
gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:4560281/76‑1 (MQ=255)
cTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg < 1:3900820/76‑1 (MQ=255)
cTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg < 2:7348455/76‑1 (MQ=255)
cTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg < 1:5525208/76‑1 (MQ=255)
tAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 2:7042142/76‑1 (MQ=255)
tAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 2:8632926/76‑1 (MQ=255)
tAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 2:5255243/76‑1 (MQ=255)
aaaCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt < 2:8770220/76‑1 (MQ=255)
aaaCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt > 1:5924425/1‑76 (MQ=255)
aCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttg < 2:3475637/76‑1 (MQ=255)
aCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttg < 2:6228397/76‑1 (MQ=255)
gCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt < 2:4686128/76‑1 (MQ=255)
cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg > 2:51623/1‑76 (MQ=255)
cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg < 2:8043714/76‑1 (MQ=255)
cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg < 2:9060441/76‑1 (MQ=255)
cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg < 2:2952190/76‑1 (MQ=255)
aaTATCAAAACGACCTGTGCCCATAGATTTCATGTGAGGTCGAAACCCGGCCTCAGTTCTGGCGGAGGGTTGTTgg < 2:1414446/76‑1 (MQ=255)
aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:2683532/76‑1 (MQ=255)
aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:8462993/76‑1 (MQ=255)
aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:5070660/76‑1 (MQ=255)
aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:1559067/76‑1 (MQ=255)
aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 1:4158874/76‑1 (MQ=255)
tatCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAg > 2:2301817/1‑76 (MQ=255)
tCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGc < 2:8897656/76‑1 (MQ=255)
taaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:1420528/2‑76 (MQ=255)
cAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:2907519/1‑76 (MQ=255)
cAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:7316145/1‑76 (MQ=255)
cAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:8400483/1‑76 (MQ=255)
aaaaCGAACCGTGCACATAAATTTAATGTGATGTCTAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg < 2:3881734/76‑1 (MQ=255)
aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg < 2:3415701/76‑1 (MQ=255)
aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg > 2:692727/1‑76 (MQ=255)
aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg > 2:1444056/1‑76 (MQ=255)
aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg > 2:2358861/1‑76 (MQ=255)
aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg < 2:4450523/76‑1 (MQ=255)
aaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCa < 1:192540/76‑1 (MQ=255)
aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg > 1:4431954/1‑76 (MQ=255)
aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg > 1:3738735/1‑76 (MQ=255)
aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg < 2:5875355/76‑1 (MQ=255)
aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg > 1:908379/1‑76 (MQ=255)
|
ATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGA > REL606/4593871‑4594016
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
