Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A95 F1 I1 R1
|
16 |
38.4 |
2546724 |
96.0% |
2444855 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,999,913 |
A→G |
Q406R (CAG→CGG) |
ubiB → |
putative ubiquinone biosynthesis protein UbiB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,999,913 | 0 | A | G | 93.8%
| 175.3
/ 1.6
| 48 | Q406R (CAG→CGG) | ubiB | putative ubiquinone biosynthesis protein UbiB |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (21/24); total (24/24) |
| Fisher's exact test for biased strand distribution p-value = 2.34e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
TACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACAGCG > REL606/3999844‑3999983
|
tACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAAc < 1:992945/76‑1 (MQ=255)
tACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGCGTTACTCCCGAAAAc > 1:717584/1‑76 (MQ=255)
aCTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAAcc > 1:1096843/1‑76 (MQ=255)
aCTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAAcc > 2:1046075/1‑76 (MQ=255)
tGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCt < 1:1124157/76‑1 (MQ=255)
gTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTg < 1:1188822/76‑1 (MQ=255)
ttAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGc < 2:1100882/76‑1 (MQ=255)
ttAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGc > 2:59638/1‑76 (MQ=255)
aaTCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGctct > 1:62654/1‑76 (MQ=255)
tCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTAt > 2:812066/1‑76 (MQ=255)
cTGTTTAATACGGCGCGTCGCTTCAATAGGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATg < 1:658344/76‑1 (MQ=255)
tttAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCg > 2:787868/1‑76 (MQ=255)
aaTACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAg < 2:281110/76‑1 (MQ=255)
tACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAggg < 1:1266732/76‑1 (MQ=255)
tACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAggg > 1:287995/1‑76 (MQ=255)
cGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGt > 2:1229160/1‑76 (MQ=255)
gcgcGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAg < 2:1002140/76‑1 (MQ=255)
cgcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAgg < 1:265822/76‑1 (MQ=255)
cgcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAgg > 2:423771/1‑76 (MQ=255)
cgcgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAgg < 2:560152/76‑1 (MQ=255)
cgTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGAc < 1:1240039/76‑1 (MQ=255)
cgTCGCTTAAATAGGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGAc < 1:656882/76‑1 (MQ=255)
ttCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGc < 2:81465/76‑1 (MQ=255)
atatGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTa > 2:502455/1‑76 (MQ=255)
atatGGAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTAGAAGGGGTAGGACGCCAGCTTTa > 2:629767/1‑76 (MQ=255)
atGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATc > 2:70879/1‑76 (MQ=255)
ggAAGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCg < 1:1208010/76‑1 (MQ=255)
aaGTGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCa < 1:970034/76‑1 (MQ=255)
tGCAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCt > 2:416193/1‑76 (MQ=255)
cAGCCGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCg > 1:1212084/1‑76 (MQ=255)
cGCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGAttt > 2:427468/1‑76 (MQ=255)
gCAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTa < 1:6388/76‑1 (MQ=255)
cAACTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTAt > 2:391775/1‑76 (MQ=255)
aaCTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATg > 1:28313/1‑76 (MQ=255)
aaCTGGTGTTACTCCAGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATg > 2:621467/1‑76 (MQ=255)
aCTGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATgg < 1:323880/76‑1 (MQ=255)
ttGGTGTTGCTCCGGAAGGCCCTGCTCTATGTCGAGGGGGTAGGGGGCCAGCTTTATCCGCAGCTCGATTTATGGa < 1:599042/75‑1 (MQ=255)
tGGTGTTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGaa < 1:519087/76‑1 (MQ=255)
gtgtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGaaaa < 2:1190652/76‑1 (MQ=255)
tgtTACTCGAGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAc < 1:231887/76‑1 (MQ=255)
tgtTACTCCGGAAATCCCTGCGCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAc < 1:719469/76‑1 (MQ=255)
tgtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGCTTTATGGAAAAc > 1:286918/1‑76 (MQ=255)
tgtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAc < 1:183512/76‑1 (MQ=255)
tgtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAc < 1:958527/76‑1 (MQ=255)
tgtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAc < 1:895396/76‑1 (MQ=255)
tgtTACTCCAGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAc > 2:48335/1‑76 (MQ=255)
gtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAAcn < 1:1097769/76‑2 (MQ=255)
gtTACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACa < 1:1015954/76‑1 (MQ=255)
ttACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACAg > 2:1161918/1‑76 (MQ=255)
tACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACAGc > 1:347761/1‑76 (MQ=255)
tACTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACAGc > 1:431224/1‑76 (MQ=255)
aCTCCGGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACAGCg > 2:1008390/1‑76 (MQ=255)
|
TACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTATGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAGCTCGATTTATGGAAAACAGCG > REL606/3999844‑3999983
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
