Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F1 I1 R1
|
14 |
43.4 |
2705329 |
98.7% |
2670159 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,069,043 |
G→A |
S472S (AGC→AGT) |
pitB ← |
phosphate transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,069,043 | 0 | G | A | 100.0%
| 114.6
/ NA
| 32 | S472S (AGC→AGT) | pitB | phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/16); total (16/16) |
GCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACGAGAGGAC > REL606/3068970‑3069114
|
gCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACt < 1:774895/76‑1 (MQ=255)
gCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTa < 2:729883/76‑1 (MQ=255)
cAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTAACGttt > 1:686176/1‑76 (MQ=255)
gTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTa > 1:933454/1‑76 (MQ=255)
ccaccaGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTa > 1:75336/1‑76 (MQ=255)
accaGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAAc > 2:748256/1‑76 (MQ=255)
gaaaaaaaAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCAc < 1:363917/76‑1 (MQ=255)
aaagaaaAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCaac > 1:1142652/1‑74 (MQ=255)
aaagaaaAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCAcc > 2:889688/1‑76 (MQ=255)
aaagaaaAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCAcc > 1:1142664/1‑76 (MQ=255)
aaaaaaaaTTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCAccg < 2:310307/73‑1 (MQ=255)
tGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTccacc < 1:295971/76‑1 (MQ=255)
gccgccGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTccacca < 1:397594/76‑1 (MQ=255)
gccgccGGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTccacca < 1:1238990/76‑1 (MQ=255)
cccgcctGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTccacca < 2:413504/75‑1 (MQ=255)
cgccgGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTATAACCCACCGCCGTccaccacc > 2:886147/1‑76 (MQ=255)
cgccgGTAAAGTAAAAACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTATAACCCACCGCCGTcccccacc > 2:886161/1‑76 (MQ=255)
cgGTAAAGTAAATACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATc < 2:1227270/76‑1 (MQ=255)
taaagtaaaTACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTc > 1:204070/1‑76 (MQ=255)
aagtaaaTACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTccc > 2:505579/1‑76 (MQ=255)
taaaTACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGc > 1:281890/1‑76 (MQ=255)
aaaTACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCa > 1:702668/1‑76 (MQ=255)
aaaTACCCACGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCa < 2:519491/76‑1 (MQ=255)
cGCCATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGAc < 2:426880/76‑1 (MQ=255)
gCCATCAGGATACTGGTTACCGTTTTACGCTATAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACg < 1:886659/76‑1 (MQ=255)
gCCATCAGGATACTGGTTACCGTTTTACGCTATAACCCACCGCCGTCCACCAACATTGTCCCTGCAACTGCAGACg < 1:886673/76‑1 (MQ=255)
ccATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACga > 2:132487/1‑76 (MQ=255)
ccATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACga > 1:458777/1‑76 (MQ=255)
cATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACgag < 2:310790/76‑1 (MQ=255)
cATCAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACgag < 1:74136/76‑1 (MQ=255)
cAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACGAGAgg < 1:1092982/76‑1 (MQ=255)
cAGGATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACGAGAgg < 1:1142234/76‑1 (MQ=255)
ggATACTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACGAGAGGAc > 2:499005/1‑76 (MQ=255)
|
GCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCTGGTTACCGTTTTACGCTGTAACCCACCGCCGTCCACCACCATCGTCCCTGCAACTGCAGACGAGAGGAC > REL606/3068970‑3069114
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
