Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A142 F1 I1 R1
|
21 |
42.6 |
2691336 |
96.3% |
2591756 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,096,436 |
C→T |
A136T (GCA→ACA) |
sufI ← |
repressor protein for FtsI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,096,436 | 0 | C | T | 100.0%
| 108.5
/ NA
| 31 | A136T (GCA→ACA) | sufI | repressor protein for FtsI |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/12); total (19/12) |
ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCA > REL606/3096363‑3096507
|
aTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTgtgt < 1:350723/76‑1 (MQ=255)
aTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTgtgt < 1:350737/76‑1 (MQ=255)
tCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTc > 1:1024861/1‑76 (MQ=255)
aGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATTCCACAGTGTAGCTGTGTTCTGGCGac > 2:666196/1‑75 (MQ=255)
gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATg < 2:1163835/76‑1 (MQ=255)
ccGTTATAGACCTGCTGAGCCGTGAGGTNCGGAGTATTGGCGTGATACTANAGAGTAGCTGTGTTCTGGCGAATgg > 1:193161/1‑76 (MQ=255)
cGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATggg > 2:1219697/1‑76 (MQ=255)
gTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTATTCTGGCGAATGGGc > 1:1101411/1‑76 (MQ=255)
ttATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCa > 1:689242/1‑76 (MQ=255)
ttATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCa > 2:749297/1‑76 (MQ=255)
ataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGt < 2:1194665/76‑1 (MQ=255)
ataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGt > 1:365611/1‑76 (MQ=255)
taGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTa < 2:1281802/76‑1 (MQ=255)
ctgctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCCAATGGGCAGTACGGGt > 1:476953/1‑76 (MQ=255)
tgctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTg < 2:279814/76‑1 (MQ=255)
gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGc > 1:663417/1‑76 (MQ=255)
gAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCa > 1:1043278/1‑76 (MQ=255)
aGCTGTGCAGTTGGGAGTATTGGCGTGATACCACAGAGCAGCAGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAg < 2:871562/76‑1 (MQ=255)
aGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTTGCGAATGGGCAGTACGGGTGCCCAg < 2:718442/76‑1 (MQ=255)
ccGTGCGGTTGGNAGNATTGGCGTGATACCACAGNGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc < 1:1093852/76‑1 (MQ=255)
cGTGCGGTTGGGAGTGTTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa > 1:126084/1‑76 (MQ=255)
tGCGGGTGGGAGTATTGGCGTGATACCACAGAGTAGCGATGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGc < 1:1114183/76‑1 (MQ=255)
ggANTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCg > 1:1199889/1‑76 (MQ=255)
aGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc > 2:631747/1‑76 (MQ=255)
ggCGTGATGCCACAGAGTAGCTGTGTTCTGGCGGATGGTCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc < 1:381292/76‑1 (MQ=255)
ggCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc < 2:582228/76‑1 (MQ=255)
ggCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc > 2:911887/1‑76 (MQ=255)
ggCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc < 1:352274/76‑1 (MQ=255)
gCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGAAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATcc < 1:33988/76‑1 (MQ=255)
tGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg < 1:992835/76‑1 (MQ=255)
aTACCACTGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc < 2:1128608/76‑1 (MQ=255)
aTACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc > 1:745832/1‑76 (MQ=255)
ccACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGa > 2:336258/1‑76 (MQ=255)
agTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGccc > 2:317657/1‑76 (MQ=255)
gTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCa > 1:403594/1‑76 (MQ=255)
aGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATc > 1:931845/1‑76 (MQ=255)
gCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCa < 2:61420/76‑1 (MQ=255)
|
ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCA > REL606/3096363‑3096507
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
