breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 86,384 C→A intergenic (‑51/+42) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 433,711 T→G I69S (ATC→AGC)  hupB → HU, DNA‑binding transcriptional regulator, beta subunit
RA 474,520 Δ1 bp coding (786/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 648,276 G→A R208C (CGC→TGC)  mrdB ← cell wall shape‑determining protein
RA 1,080,405 A→G Y27C (TAC→TGC)  yccE → hypothetical protein
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,658,770 T→G N200K (AAT→AAG ydgB → short chain dehydrogenase
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,027,586 C→T A29T (GCC→ACC)  ECB_02827 ← Polysialic acid transport ATP‑binding KpsT
RA 3,096,436 C→T A136T (GCA→ACA)  sufI ← repressor protein for FtsI
RA 3,145,823 G→A intergenic (+154/‑41) dnaG → / → rpoD DNA primase/RNA polymerase sigma factor
RA 3,488,817 C→T M130I (ATG→ATA glpR ← DNA‑binding transcriptional repressor
RA 3,535,089 (A)6→5 coding (9/597 nt) yhhF → predicted methyltransferase
MC JC 3,619,789 (GAC)4→3 coding (2309‑2311/2340 nt) yhjN ← regulator of cellulose synthase, cyclic di‑GMP binding
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
RA 3,893,551 +G intergenic (+6/‑50) kup → / → insJ‑5 potassium transporter/IS150 hypothetical protein
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,162,569 A→C I572L (ATC→CTC)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546989–547700 593383 45684–46395 14 [12] [11] 13 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593551 619828–619116 25566–26278 13 [12] [11] 14 [fepA]–[insA‑8] [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]