Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F1 I1 R1
|
14 |
43.4 |
2705329 |
98.7% |
2670159 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,414,552 |
T→G |
I176M (ATT→ATG) |
crp → |
DNA‑binding transcriptional dual regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,414,552 | 0 | T | G | 100.0%
| 146.4
/ NA
| 36 | I176M (ATT→ATG) | crp | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/19); total (17/19) |
CAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACA > REL606/3414479‑3414623
|
cAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGt > 1:755627/1‑76 (MQ=255)
ccAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGtt < 2:497292/76‑1 (MQ=255)
gCTGTGACTCACCCGGCCGGTATGCAAATCAAAATGACCCGTCAGGAAATTGGTCAGAGGGTCGGCTGTTCTCGTg < 1:1140660/76‑1 (MQ=255)
gCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTg < 1:1044685/76‑1 (MQ=255)
gCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTg < 2:184907/76‑1 (MQ=255)
gCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTg < 1:1140639/76‑1 (MQ=255)
gACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAAcc > 1:661920/1‑76 (MQ=255)
aCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCg > 1:82675/1‑76 (MQ=255)
aCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCg > 1:72261/1‑76 (MQ=255)
cACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTgg < 2:482430/76‑1 (MQ=255)
cGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACg < 1:437966/76‑1 (MQ=255)
cGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACg > 2:928048/1‑76 (MQ=255)
gTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt > 1:748292/1‑76 (MQ=255)
gTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt > 1:981933/1‑76 (MQ=255)
aTGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTTTGa < 2:516843/76‑1 (MQ=255)
tGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa > 1:107703/1‑76 (MQ=255)
gCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAg > 1:1009966/1‑76 (MQ=255)
aaaTCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAt < 2:768333/76‑1 (MQ=255)
aaTCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATg > 1:1305004/1‑76 (MQ=255)
tCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt > 1:366336/1‑76 (MQ=255)
aaaTTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa < 2:546124/76‑1 (MQ=255)
aaTTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGaa < 2:1107468/76‑1 (MQ=255)
aTTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAg > 2:120398/1‑76 (MQ=255)
aCCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc > 1:625123/1‑76 (MQ=255)
cccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa > 2:590353/1‑76 (MQ=255)
cccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa < 1:905616/76‑1 (MQ=255)
cccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa < 1:905629/76‑1 (MQ=255)
ccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAg < 1:514359/76‑1 (MQ=255)
ccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGGGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAg < 1:514351/76‑1 (MQ=255)
gTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa > 1:536192/1‑76 (MQ=255)
cAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc > 2:575862/1‑76 (MQ=255)
aaaTTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt < 1:1057204/76‑1 (MQ=255)
ttGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtctc > 1:1161642/1‑76 (MQ=255)
cAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcac < 2:747835/76‑1 (MQ=255)
aGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca < 2:261575/76‑1 (MQ=255)
aGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca < 1:1344218/76‑1 (MQ=255)
|
CAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACA > REL606/3414479‑3414623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
