Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A35 F1 I1 R1
|
21 |
46.3 |
3071142 |
93.0% |
2856162 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,333,815 |
T→G |
pseudogene (174/189 nt) |
yciX → |
predicted protein; b4522(b4574) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,333,815 | 0 | T | G | 100.0%
| 120.9
/ NA
| 31 | pseudogene (174/189 nt) | yciX | predicted protein; b4522(b4574) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/14); total (17/14) |
CGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACA > REL606/1333746‑1333882
|
cgtTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCg > 1:42261/1‑76 (MQ=255)
ttATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGaa > 2:1092850/1‑76 (MQ=255)
ttATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGaa > 2:222434/1‑76 (MQ=255)
aTTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAAc < 1:669156/76‑1 (MQ=255)
ccAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACaat > 1:1238535/1‑76 (MQ=255)
cAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACaata < 1:760807/76‑1 (MQ=255)
cAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACaata < 2:498211/76‑1 (MQ=255)
cgacTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTa > 1:1248638/1‑76 (MQ=255)
tGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTatcat < 1:506827/76‑1 (MQ=255)
tGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTatcat > 2:1340564/1‑76 (MQ=255)
ggCAACTAACATCGCAGCAGCAAGCCTTTATAGAACGGTTTGCTGAAGATGAGCAGCCGAAACAATAATTatcatc < 1:925885/76‑1 (MQ=255)
cTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCt > 2:660196/1‑76 (MQ=255)
aaCATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCtta > 1:207823/1‑76 (MQ=255)
aaCATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCtta > 1:112630/1‑76 (MQ=255)
aTCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCttatta > 1:679773/1‑76 (MQ=255)
cagcagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCa < 2:417652/76‑1 (MQ=255)
cagcagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCa > 1:971845/1‑76 (MQ=255)
agcagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCAt < 1:204092/76‑1 (MQ=255)
cagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCAttt > 1:1088487/1‑76 (MQ=255)
cagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCAttt < 1:138067/76‑1 (MQ=255)
cagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCAttt < 2:1356373/76‑1 (MQ=255)
cagcaAGCCTTTATAGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCAttt < 2:1052437/76‑1 (MQ=255)
tataGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTa < 1:918244/76‑1 (MQ=255)
ataGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTaa > 2:1350620/1‑76 (MQ=255)
taGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTaaa > 2:410580/1‑76 (MQ=255)
aGAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAg < 1:991892/76‑1 (MQ=255)
gAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAgg < 1:968450/76‑1 (MQ=255)
gAACTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAgg < 1:1218810/76‑1 (MQ=255)
aCTGTTTGCTGAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGc > 1:474326/1‑76 (MQ=255)
gAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATaca > 1:492014/1‑76 (MQ=255)
gAAGATGAGCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATaca > 2:896408/1‑76 (MQ=255)
|
CGTTATTCCAGACGACTGGCAACTAACATCGCAGCAGCAAGCCTTTATAGAACTGTTTGCTGAAGATGATCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACA > REL606/1333746‑1333882
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
