Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
18 |
38.4 |
2430560 |
95.7% |
2326045 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,345,210 |
(T)8→7 |
intergenic (‑98/+137) |
yciR ← / ← rnb |
modulator of Rnase II stability/exoribonuclease II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,345,203 | 0 | T | . | 96.5%
| 128.2
/ ‑2.7
| 29 | intergenic (‑91/+144) | yciR/rnb | modulator of Rnase II stability/exoribonuclease II |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (8/20); total (9/20) |
| Fisher's exact test for biased strand distribution p-value = 3.10e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
TAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAA > REL606/1345130‑1345274
|
tAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGttt < 2:39392/76‑1 (MQ=255)
aaCAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGtttt < 1:908481/76‑1 (MQ=255)
aGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGttttttt < 2:601813/76‑1 (MQ=255)
cGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGtttttttat < 2:1131862/76‑3 (MQ=255)
ggAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAAAGCAATGtttttttttt > 1:472974/1‑74 (MQ=255)
gTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTatatat < 1:285867/76‑1 (MQ=255)
tCCGCGGTTAAATANAAGGAACAACATGACTTACATGAAATTTACGGCGGCTAAACGCATCG‑TCTTTTTatatatn < 1:1044210/76‑2 (MQ=255)
cgcgGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATAtattta < 1:694821/76‑1 (MQ=255)
ggTTAAATAAANGGNACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑GCTTTTTATCTatttattt < 2:190847/76‑1 (MQ=255)
ttAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGt < 2:598913/76‑1 (MQ=255)
aaatagaAGGAACAACATGACTTACATGAAATTAACGGCGGGCACAAGATTTGTTTTTTTT‑TATTTTTTTTTGtta > 1:80092/1‑74 (MQ=255)
taaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATGTATTTGTAATcc < 2:46706/76‑1 (MQ=255)
aaaGGAACAACATGACTTACATGAAATTAACGGCGGCTAAATGCATTG‑TTTTTTNATATATTTTTNTGTTATCCAg < 2:1196116/76‑1 (MQ=255)
gAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGtttt < 1:708894/76‑1 (MQ=255)
gAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGtttt < 1:312649/76‑1 (MQ=255)
gAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGtttt < 1:333301/76‑1 (MQ=255)
aacaTGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGaa < 2:431445/76‑1 (MQ=255)
aacaTGACTTACATGAAATTAACGGCGGCCAAAAGCATTGGTTTTTTTTTTTTTTATTTTTTAATCAATTTTTGGa > 1:347697/1‑76 (MQ=16)
aTGACTTACATGAAATTAACGGCGGCTAAAAGCATTGTTTTTTTTTTTTTTTTTTTTTTATTCCGTTTTTGGaaaa > 1:924612/1‑76 (MQ=255)
ttACATGAAATNAANGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCTAGTTTTGGAAATACGCCa < 2:185605/76‑1 (MQ=255)
aTGAAATTAACGGCGGCTANANGCATTG‑TNTTTTTATATATTTGTTTGTAANCCNGTTTTGGAAAAACGCCAGttt < 2:175379/76‑1 (MQ=255)
tGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtttt < 2:376937/76‑1 (MQ=255)
gAAATTAACGGNGGNTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTCGGAAAAACTCCAGTTTTc < 2:186277/76‑1 (MQ=255)
aaaTTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCa < 2:832670/76‑1 (MQ=255)
ggcggcTAAACGGATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAg > 2:48039/1‑76 (MQ=255)
cggcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTc > 1:557071/1‑76 (MQ=255)
ggcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCa > 1:909298/1‑76 (MQ=255)
gcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg > 1:408785/1‑76 (MQ=255)
gcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg > 2:510408/1‑76 (MQ=255)
tAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGtt > 2:86763/1‑76 (MQ=255)
tAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGtt < 1:705189/76‑1 (MQ=255)
aaaCGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa > 1:899820/1‑76 (MQ=255)
aaaCGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa < 1:625659/76‑1 (MQ=255)
aaaCCCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGGAAGTCAGTTa > 1:1114423/1‑76 (MQ=255)
cGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaa < 2:1116924/76‑1 (MQ=255)
cATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaaaa < 1:615269/76‑1 (MQ=255)
|
TAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAA > REL606/1345130‑1345274
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
