mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 101,810 | G→A | V202M (GTG→ATG) | ftsW → | integral membrane protein involved in stabilizing FstZ ring during cell division |
| MC JC | 475,062 | Δ4 bp | coding (241‑244/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| JC | 990,173 | +CGT | coding (321/780 nt) | ycbC ← | conserved inner membrane protein |
| JC JC | 1,305,487 | IS150 (–) +3 bp | coding (751‑753/1461 nt) | cls ← | cardiolipin synthetase |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| RA | 1,345,210 | (T)8→7 | intergenic (‑98/+137) | yciR ← / ← rnb | modulator of Rnase II stability/exoribonuclease II |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| RA | 2,295,476 | A→C | *85C (TGA→TGC) | yfaE → | predicted 2Fe‑2S cluster‑containing protein |
| RA | 3,145,984 | A→T | I41F (ATC→TTC) | rpoD → | RNA polymerase sigma factor |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| MC JC | 3,894,997 | Δ6,991 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
| RA | 3,911,997 | (A)6→5 | intergenic (‑198/‑125) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| RA | 4,096,926 | A→G | L216P (CTG→CCG) | glpF ← | glycerol facilitator |
| RA | 4,166,437 | C→T | P493S (CCG→TCG) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| RA | 4,273,299 | A→T | intergenic (+227/‑115) | nrfG → / → gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | REL606 | 546985–547700 | 593372 | 45673–46388 | 11 [9] | [10] 11 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
| * | * | ÷ | REL606 | 593565 | 619839–619116 | 25552–26275 | 11 [10] | [9] 11 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |
| * | * | ÷ | REL606 | 2842143–2842194 | 2842242–2842211 | 18–100 | 11 [10] | [10] 11 | [metW]–[metV] | [metW],[metV] |