Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A75 F1 I1 R1
|
16 |
50.8 |
3316832 |
95.7% |
3174208 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,345,210 |
(T)8→7 |
intergenic (‑98/+137) |
yciR ← / ← rnb |
modulator of Rnase II stability/exoribonuclease II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,345,203 | 0 | T | . | 96.5%
| 133.2
/ ‑3.8
| 29 | intergenic (‑91/+144) | yciR/rnb | modulator of Rnase II stability/exoribonuclease II |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (6/22); total (7/22) |
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAA > REL606/1345128‑1345272
|
gTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGt < 2:749027/76‑1 (MQ=255)
tAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGttt < 2:789912/76‑1 (MQ=255)
aaCAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGGTAAACGCATCGtttt > 2:726520/1‑76 (MQ=255)
aaCAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGtttt < 2:1164029/76‑1 (MQ=255)
cAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGtttttt < 2:558598/76‑1 (MQ=255)
cGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTGAACGCGGTGtgttttttt > 2:1565666/1‑75 (MQ=255)
cGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGttttttttt > 1:419927/1‑75 (MQ=255)
gAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGtttttttttat > 1:1122664/1‑73 (MQ=255)
tCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATAtatt < 1:863833/76‑1 (MQ=255)
tCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATAtatt < 1:1439971/76‑1 (MQ=255)
cgcgGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATAtattta < 1:331986/76‑1 (MQ=255)
cgcgGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATAtattta < 1:1224854/76‑1 (MQ=255)
ggTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTT‑TATatttattt > 2:623142/1‑76 (MQ=255)
taaataaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTa < 2:1092046/76‑1 (MQ=255)
taaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATcc < 2:366652/76‑1 (MQ=255)
taaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATcc < 2:236449/76‑1 (MQ=255)
taaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATcc < 2:1435028/76‑1 (MQ=255)
taaaAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATcc < 2:1283911/76‑1 (MQ=255)
taaaAGGAACAACATGACTTACATGAAATTAACGGCGGCCAAACGCCTT‑ATTTTTTTATTTATTTATTTTTAATcc > 2:817546/1‑76 (MQ=255)
aaaaGGAACAACATGACTTACATGAAATTAACGGCGGCTAAAAGCATTGCTTTTTTT‑TATATTTTTTTGTAATTCa > 2:1093848/1‑76 (MQ=255)
aaaGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAg < 2:107859/76‑1 (MQ=255)
acaacaTGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTgg < 2:625030/76‑1 (MQ=255)
tGACTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAAc < 1:807204/76‑1 (MQ=255)
aCTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGc < 1:1481337/76‑1 (MQ=255)
aCTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGc < 1:188598/76‑1 (MQ=255)
aCTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGc < 1:436674/76‑1 (MQ=255)
cTTACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGcc < 1:1216618/76‑1 (MQ=255)
ttACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCa < 1:1331128/76‑1 (MQ=255)
tACATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg < 2:583898/76‑1 (MQ=255)
cATGAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtt < 1:163431/76‑1 (MQ=255)
tGAAATTAACGGCGGCTAAACGCATT‑TTTTTTTTTTTTTTTTTTTTTTAATTCAGTTTTTGTAAAAAACCAGtttt > 1:844466/1‑76 (MQ=255)
gAAATTAACGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTc < 1:679333/76‑1 (MQ=255)
aCGGCGGCTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGaa < 1:1201514/76‑1 (MQ=255)
gcggcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGt < 2:770194/76‑1 (MQ=255)
gcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg > 1:107206/1‑76 (MQ=255)
gcTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg > 1:692001/1‑76 (MQ=255)
cTAAACGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGt > 1:804747/1‑76 (MQ=255)
aaaCGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa > 2:860347/1‑76 (MQ=255)
aaaCGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa > 1:493974/1‑76 (MQ=255)
cGCATTG‑TTTTTTTATATATTTATTTGTAATCCAGTTTTGGCACAACGCCAGTTTTCAAACGAAAGTCAGTTaaaa > 2:570717/1‑76 (MQ=255)
|
GTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAA > REL606/1345128‑1345272
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
