Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A84 F1 I1 R1
|
17 |
47.9 |
3074446 |
96.0% |
2951468 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,356,628 |
C→T |
A310T (GCC→ACC) |
ycjJ ← |
putrescine importer |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,356,628 | 0 | C | T | 94.4%
| 125.1
/ ‑4.2
| 36 | A310T (GCC→ACC) | ycjJ | putrescine importer |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (12/22); total (13/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGCATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGAACAGCTTACC > REL606/1356555‑1356700
|
tGCACATAGCCAAACACGCGCCCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTAt > 1:949813/1‑76 (MQ=255)
acaTAGCCAAACACGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTAtgtg < 2:494163/76‑1 (MQ=255)
acaTAGCCAAACACGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTAtgtg > 2:111964/1‑76 (MQ=255)
tAGCCAAACACGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGAGACACGCTGGCATGTGagg > 2:1347335/1‑76 (MQ=255)
cAAACACGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGaggccag < 2:1336745/76‑1 (MQ=255)
acacGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGaggccaggcc < 2:969913/76‑1 (MQ=255)
acacGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGaggccaggcc < 2:396843/76‑1 (MQ=255)
acacGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGaggccaggcc > 2:826920/1‑76 (MQ=255)
acGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGgccaggccag < 2:706049/76‑1 (MQ=255)
cgcTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACg > 1:1392298/1‑76 (MQ=255)
gcTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGc > 1:587269/1‑76 (MQ=255)
cTCCGAAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCt < 2:635358/76‑1 (MQ=255)
cacaTTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTc > 1:873932/1‑76 (MQ=255)
cacaTGGTCGCGCCCCATCACATACAGCAGACGTGCCACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTc < 2:992781/76‑1 (MQ=255)
caTTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTcac < 2:702639/76‑1 (MQ=255)
aTTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTcaca < 1:1054705/76‑1 (MQ=255)
tGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACaaa > 1:1489559/1‑76 (MQ=255)
tGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACaaa < 2:1249203/76‑1 (MQ=255)
ccATCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGcn < 1:184104/76‑2 (MQ=255)
aTCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCaaa > 2:321537/1‑76 (MQ=255)
aTCACATACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCaaa < 1:1347335/76‑1 (MQ=255)
caTACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGaa > 2:1280364/1‑76 (MQ=255)
aTACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGaaa < 1:185979/76‑1 (MQ=255)
aTACAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGaaa < 2:756238/76‑1 (MQ=255)
aCAGCGGGCGTGACCCGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAAt < 1:272491/76‑1 (MQ=255)
aCAGCAGACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAAt < 2:839179/76‑1 (MQ=255)
agcagACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCg < 1:1322855/76‑1 (MQ=255)
gcagACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGa < 1:691518/76‑1 (MQ=255)
cagACGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGAc < 2:1267169/76‑1 (MQ=255)
gACGTGACACGCTGGCATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTg < 1:1362036/76‑1 (MQ=255)
aCGTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTgg > 2:1145781/1‑76 (MQ=255)
gTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGaa < 2:781245/76‑1 (MQ=255)
gTGACACGCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGaa < 1:1318002/76‑1 (MQ=255)
gCTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGAACAGCTTa < 2:315715/76‑1 (MQ=255)
cTGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGAACAGCTTAc > 1:1347102/1‑76 (MQ=255)
tGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGAACAGCTTAcc > 1:637227/1‑76 (MQ=255)
tGGTATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGAACAGCTTAcc > 1:131553/1‑76 (MQ=255)
|
TGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCGCGCCCCATCACATACAGCAGACGTGACACGCTGGCATGTGAGGCCAGGCCAGACGCTAACGTGTTCACAAACGTGGTGCAAAGGAAAATCGACTGGAACAGCTTACC > REL606/1356555‑1356700
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
