Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A35 F1 I1 R1
|
21 |
46.3 |
3071142 |
93.0% |
2856162 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,377,568 |
C→A |
G74V (GGT→GTT) |
rpsC ← |
30S ribosomal protein S3 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,377,568 | 0 | C | A | 100.0%
| 111.8
/ NA
| 29 | G74V (GGT→GTT) | rpsC | 30S ribosomal protein S3 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/13); total (16/13) |
GGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGAC > REL606/3377496‑3377639
|
ggAACGCCAGGGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCg < 1:720550/76‑1 (MQ=255)
gAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCgg > 2:673466/1‑76 (MQ=255)
aaCGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCggg > 2:820569/1‑76 (MQ=255)
cGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCg < 2:749401/76‑1 (MQ=255)
aGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCa > 2:502907/1‑76 (MQ=255)
aGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCa < 1:811718/76‑1 (MQ=255)
aTGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAgtgt > 2:1310396/1‑76 (MQ=255)
gTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGa > 2:120449/1‑76 (MQ=255)
cGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATg > 1:1445727/1‑76 (MQ=255)
tACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGtt > 1:1277094/1‑76 (MQ=255)
ccTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACg > 2:82715/1‑76 (MQ=255)
ccTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACg < 1:1435810/76‑1 (MQ=255)
ttACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGa > 1:995200/1‑76 (MQ=255)
tACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGAt > 2:224495/1‑76 (MQ=255)
tACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGAt > 2:1104032/1‑76 (MQ=255)
gctgTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGct < 2:947218/73‑1 (MQ=255)
gCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGct < 2:320044/76‑1 (MQ=255)
tttttCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTa > 1:883689/1‑76 (MQ=255)
tttttCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTa < 2:736452/76‑1 (MQ=255)
cTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTAGACGGATGCTCTTAGCCgg < 2:578010/76‑1 (MQ=255)
cTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCgg > 1:669773/1‑76 (MQ=255)
cGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACg > 1:401026/1‑76 (MQ=255)
cGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACg < 1:1125725/76‑1 (MQ=255)
cGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACg < 1:1065412/76‑1 (MQ=255)
cTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTc < 1:1149216/76‑1 (MQ=255)
aCCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATa < 1:401963/76‑1 (MQ=255)
ttACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATAc > 1:1354333/1‑76 (MQ=255)
tACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACg > 2:1412689/1‑76 (MQ=255)
gataacgataacCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGAt < 1:963889/76‑1 (MQ=255)
gataacgataacCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGAt < 1:831554/76‑1 (MQ=255)
gATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGAc < 2:508663/76‑1 (MQ=255)
|
GGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGAC > REL606/3377496‑3377639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
