Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A97 F1 I1 R1
|
18 |
21.9 |
1431356 |
95.6% |
1368376 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,028,373 |
A→T |
L30Q (CTG→CAG) |
ECB_02828 ← |
Polysialic acid transport protein kpsM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,028,373 | 0 | A | T | 97.4%
| 150.6
/ ‑5.1
| 39 | L30Q (CTG→CAG) | ECB_02828 | Polysialic acid transport protein kpsM |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (20/18); total (20/19) |
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTGGACTTCAA > REL606/3028300‑3028445
|
cATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGa > 2:626762/1‑76 (MQ=255)
aTAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGAc > 2:51882/1‑76 (MQ=255)
aTGTAACCAAAAATGCCCCACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGa > 2:497852/1‑76 (MQ=255)
aTGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGa < 1:95314/76‑1 (MQ=255)
tGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGaa < 2:690584/76‑1 (MQ=255)
gTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAAc < 2:691577/76‑1 (MQ=255)
aaCCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACtt < 2:85221/76‑1 (MQ=255)
aCCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTa < 1:689888/76‑1 (MQ=255)
aaaaTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCaa < 1:10507/76‑1 (MQ=255)
aaaaTGCCCAACAGAATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCaa > 1:384656/1‑76 (MQ=255)
aaTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAg > 2:701226/1‑76 (MQ=255)
aTGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGc > 2:695083/1‑76 (MQ=255)
gCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCgt < 2:556858/76‑1 (MQ=255)
cccAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCgtg < 2:59933/76‑1 (MQ=255)
cAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGt > 2:143714/1‑76 (MQ=255)
aTCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTAttt < 1:665122/76‑1 (MQ=255)
cAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTtct > 2:526181/1‑76 (MQ=255)
ncAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCg > 1:694124/2‑76 (MQ=255)
aaaTGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTa > 2:221291/1‑76 (MQ=255)
aaaTGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTa < 2:282550/76‑1 (MQ=255)
aaTGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAg > 1:348440/1‑76 (MQ=255)
tGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGaa < 1:25425/76‑1 (MQ=255)
tGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGaa < 2:239356/76‑1 (MQ=255)
cgcAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaat > 1:180652/1‑76 (MQ=255)
gcAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaata > 2:556435/1‑76 (MQ=255)
gcAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaata < 1:121111/76‑1 (MQ=255)
cAGAGGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaataa > 1:271751/1‑76 (MQ=255)
agagGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAtataat > 2:706387/1‑76 (MQ=255)
agagGGTTCGAGAATCGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAaataat > 1:437779/1‑76 (MQ=255)
cGCCCACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACt > 2:142207/1‑76 (MQ=255)
cccACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACttt < 2:1966/76‑1 (MQ=255)
cccACAAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACttt < 1:665795/76‑1 (MQ=255)
cacaAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCt > 1:300849/1‑76 (MQ=255)
caAATATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTgg < 2:103914/76‑1 (MQ=255)
aaTATCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTGGAc < 1:593075/76‑1 (MQ=255)
atCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTATCTGGACTTc > 2:502135/1‑76 (MQ=255)
tCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTGGACTTCa < 1:661888/76‑1 (MQ=255)
tCCCTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTGGACTTCa < 1:213205/76‑1 (MQ=255)
cccTGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTGGACTTCaa > 1:644717/1‑76 (MQ=255)
|
CATAATGTAACCAAAAATGCCCAACAGTATCAGCAAATGCGCAGAGGGTTCGAGAATCGCCCACAAATATCCCAGACGGAACTTACCAAAGCGTGTTCGTATTTCTCGTAGAAATAATGCCTCTACGGTGACTTTCTGGACTTCAA > REL606/3028300‑3028445
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
