breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 294,446 IS1 (+) +9 bp coding (190‑198/720 nt) ykgE → predicted oxidoreductase
RA 465,735 A→C Q526P (CAG→CCG)  dnaX → DNA polymerase III subunits gamma and tau
RA 473,924 A→C V461G (GTC→GGC)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 648,692 C→T R69H (CGC→CAC)  mrdB ← cell wall shape‑determining protein
RA 825,980 G→A D96N (GAT→AAT)  dinG → ATP‑dependent DNA helicase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,058,079 C→A P291P (CCG→CCT wcaI ← predicted glycosyl transferase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
MC JC 2,298,759 Δ4 bp coding (321‑324/1359 nt) glpT ← sn‑glycerol‑3‑phosphate transporter
RA 3,028,373 A→T L30Q (CTG→CAG)  ECB_02828 ← Polysialic acid transport protein kpsM
RA 3,145,853 G→A intergenic (+184/‑11) dnaG → / → rpoD DNA primase/RNA polymerase sigma factor
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,162,569 A→C I572L (ATC→CTC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,202,426 G→A A45V (GCA→GTA)  iclR ← DNA‑binding transcriptional repressor
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2035140NA (NA)7 (0.350) 4/134 2.2 20.0% coding (717/768 nt) wbbD putative glycosyltransferase WbbD
?REL606 = 2035556 28 (1.420)coding (301/768 nt) wbbD putative glycosyltransferase WbbD
* ? REL606 2038432 =23 (1.040)9 (0.460) 4/134 2.2 24.2% coding (517/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 2038941 = 36 (1.820)coding (8/822 nt) wbbB putative glycosyltransferase WbbB