Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I3 R1 53 201.3 10250884 92.1% 9441064 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,568,561 C→T A256A (GCG→GCA)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,568,5610CT99.0% 715.1 / ‑4.9 208A256A (GCG→GCA) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (96/109);  total (97/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCA  >  NC_000913/3568466‑3568657
                                                                                               |                                                                                                
aGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCAt                                                                                              <  1:1484179/100‑1 (MQ=255)
 ggTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATa                                                                                             >  1:637780/1‑100 (MQ=255)
 ggTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCCCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGCACGGGCGTGCATa                                                                                             >  1:4830479/1‑100 (MQ=255)
    tCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGCCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGc                                                                                          >  1:373458/1‑100 (MQ=255)
     cGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGcc                                                                                         <  1:2159215/100‑1 (MQ=255)
     cGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGcc                                                                                         <  1:1785622/100‑1 (MQ=255)
     cGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGcc                                                                                         <  2:2445610/100‑1 (MQ=255)
     cGCTTTCCAGTAAGCTTCCAGCGTACCAACGTCGCGACAGTACGGCGCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGcc                                                                                         <  2:1152431/100‑1 (MQ=255)
      gCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCa                                                                                        >  2:2599438/1‑100 (MQ=255)
       cTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAg                                                                                       <  2:851655/100‑1 (MQ=255)
       cTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAg                                                                                       >  2:1896372/1‑100 (MQ=255)
            cAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGGGCGGGAACGGGTGTGCATAGGCCAGACCgg                                                                                  >  2:2706752/1‑100 (MQ=255)
             aGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGc                                                                                 <  2:3786032/100‑1 (MQ=255)
              gTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCt                                                                                >  1:710259/1‑100 (MQ=255)
                 aGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCg                                                                             >  1:3662701/1‑100 (MQ=255)
                  gCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCgg                                                                            >  2:2679432/1‑100 (MQ=255)
                  gCTACCAGCGTACCCACATCGCGCCAGTACGGCTTGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCgg                                                                            <  1:1143253/100‑1 (MQ=255)
                   cTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGt                                                                           <  1:3817997/100‑1 (MQ=255)
                    ttCCAGCGTACGCATATCGCGCCAGTACAGCTCGGCATCCGGGTCGGATTGTACGGAAGAGAGCGGTAACGAGTGTGCATAGGCCAGAACAGCTTCtgtg                                                                          <  2:1077988/100‑1 (MQ=255)
                     tCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGa                                                                         >  1:1027475/1‑100 (MQ=255)
                      ccAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGAt                                                                        >  2:1267327/1‑100 (MQ=255)
                       cAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATc                                                                       >  1:107154/1‑100 (MQ=255)
                       cAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATc                                                                       >  1:3125338/1‑100 (MQ=255)
                       cAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATc                                                                       >  1:4060942/1‑100 (MQ=255)
                        aGCGTACCCACATCGCGCCAGTACGGCTCGGCGTCCGGGGCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCt                                                                      <  2:2147529/100‑1 (MQ=255)
                        aGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCt                                                                      <  1:320499/100‑1 (MQ=255)
                          cGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTg                                                                    <  2:4491724/100‑1 (MQ=255)
                           gTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTgg                                                                   <  1:2739773/100‑1 (MQ=255)
                           gTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTgg                                                                   >  2:1360147/1‑100 (MQ=255)
                            tACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTggg                                                                  <  1:3557883/100‑1 (MQ=255)
                            tACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTggg                                                                  >  2:3848040/1‑100 (MQ=255)
                            tACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTggg                                                                  <  1:1287549/100‑1 (MQ=255)
                            tACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTggg                                                                  <  1:5098260/100‑1 (MQ=255)
                              cccACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGaa                                                                >  2:1723834/1‑100 (MQ=255)
                                cacaTCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATc                                                              <  2:3685093/100‑1 (MQ=255)
                                cacaTCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATc                                                              >  2:2826621/1‑100 (MQ=255)
                                cacaTCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATc                                                              >  2:2260800/1‑100 (MQ=255)
                                cacaTCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATc                                                              >  2:120747/1‑100 (MQ=255)
                                 acaTCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCa                                                             <  1:2667210/100‑1 (MQ=255)
                                  naTCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCaa                                                            >  1:4111376/2‑100 (MQ=255)
                                   aTCGCGCCAATAGGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCCGCTTCGGTGATCTTAGGAATCaac                                                           <  2:235137/100‑2 (MQ=255)
                                    tCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAAt                                                          <  2:3987263/100‑1 (MQ=255)
                                      gcgcCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCt                                                        >  1:557659/1‑100 (MQ=255)
                                              aCGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCaaa                                                >  2:3631314/1‑100 (MQ=255)
                                               cGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAg                                               >  2:3731756/1‑100 (MQ=255)
                                               cGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAg                                               >  1:1974447/1‑100 (MQ=255)
                                                ggCTCGGCATCCGGGGCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGt                                              >  1:1535560/1‑100 (MQ=255)
                                                 gCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAACTc                                             <  1:3382465/100‑1 (MQ=255)
                                                    cGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTg                                          <  2:334941/100‑1 (MQ=255)
                                                    atctaTCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTg                                          <  1:4330527/96‑1 (MQ=255)
                                                     ggCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTgg                                         <  2:306332/100‑1 (MQ=255)
                                                      gCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGc                                        <  1:972646/100‑1 (MQ=255)
                                                      gCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGc                                        <  1:2931355/100‑1 (MQ=255)
                                                       cATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCt                                       >  1:1224441/1‑100 (MQ=255)
                                                       cATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCt                                       >  1:334966/1‑100 (MQ=255)
                                                        aTCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTg                                      >  2:3433479/1‑100 (MQ=255)
                                                        aTCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTg                                      >  1:4587940/1‑100 (MQ=255)
                                                        aTCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTg                                      >  2:567660/1‑100 (MQ=255)
                                                         tCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGACGTGGCTgg                                     <  1:5026215/100‑1 (MQ=255)
                                                           cGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAg                                   <  1:3851958/100‑1 (MQ=255)
                                                            gggTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGt                                  <  2:1873861/100‑1 (MQ=255)
                                                            gggTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGt                                  <  1:1740306/100‑1 (MQ=255)
                                                              gTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTtc                                <  2:388730/100‑1 (MQ=255)
                                                              gTCGGATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTtc                                >  2:4937880/1‑100 (MQ=255)
                                                                 ggATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCa                             >  1:3069294/1‑100 (MQ=255)
                                                                  gATTGTACGCAAGAGAGCGGGACCGGATCTGCATCGGCCAGACCGGCCTCTGCGATCTTGGGAATCAAATCTTCGCCAAAACCGTTGCTGGAGCACTCAt                            >  2:618371/1‑100 (MQ=255)
                                                                  gATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCAt                            >  2:4842015/1‑100 (MQ=255)
                                                                  gATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCAt                            >  2:1454596/1‑100 (MQ=255)
                                                                  gATTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCAt                            <  1:4889250/100‑1 (MQ=255)
                                                                   aTTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATc                           >  2:3101353/1‑100 (MQ=255)
                                                                   aTTGTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGCGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATc                           >  2:1992534/1‑100 (MQ=255)
                                                                      gTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATcgcg                        >  2:2168169/1‑100 (MQ=255)
                                                                      gTACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATcgcg                        >  2:518872/1‑100 (MQ=255)
                                                                       tACGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGa                       >  1:3239127/1‑100 (MQ=255)
                                                                        aCGCAAGAGAGCGGGAACGGGTGTGCCTAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGAt                      >  2:3524755/1‑100 (MQ=255)
                                                                        aCGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGAt                      <  2:1778299/100‑1 (MQ=255)
                                                                        aCGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGAt                      <  1:2040164/100‑1 (MQ=255)
                                                                         cGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATc                     >  2:2906916/1‑100 (MQ=255)
                                                                         cGCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATc                     >  2:1943621/1‑100 (MQ=255)
                                                                          gCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCg                    >  2:4002281/1‑100 (MQ=255)
                                                                          gCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCg                    <  1:1249513/100‑1 (MQ=255)
                                                                          gCAAGAGAGCGGGAACGGGTGTGCATAGGCCAGACCGACTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCg                    >  1:832213/1‑100 (MQ=255)
                                                                             agagagCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGtct                 <  2:4227126/100‑1 (MQ=255)
                                                                             agagagCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGtct                 <  1:2367473/100‑1 (MQ=255)
                                                                                  gCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCttcttc            >  2:3295171/1‑100 (MQ=255)
                                                                                  gCGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCttcttc            >  2:2099479/1‑100 (MQ=255)
                                                                                   cGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTcc           <  1:1773817/100‑1 (MQ=255)
                                                                                   cGGGAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTcc           >  1:4017527/1‑100 (MQ=255)
                                                                                    gggAACGGGTGTGCATAGGCCTGACTGGCTTCGGTGCTCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCca          <  2:4810910/100‑1 (MQ=255)
                                                                                    gggAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCca          <  1:2559621/100‑1 (MQ=255)
                                                                                     ggAGCGGGTGTGCATAGGCGAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCcag         >  2:4654457/1‑100 (MQ=255)
                                                                                      gAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCcagc        <  1:2858609/100‑1 (MQ=255)
                                                                                      gAACGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCcagc        <  2:3234530/100‑1 (MQ=255)
                                                                                        aCGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCcagcag      <  2:699351/100‑1 (MQ=255)
                                                                                         cGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGt     <  1:3061323/100‑1 (MQ=255)
                                                                                         cGGGTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGt     <  1:2331505/100‑1 (MQ=255)
                                                                                          gggTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGtt    <  1:1006096/100‑1 (MQ=255)
                                                                                           ggTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTc   >  2:4776174/1‑100 (MQ=255)
                                                                                           ggTGTGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTc   <  1:470078/100‑1 (MQ=255)
                                                                                            gtgtGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCa  >  1:2301092/1‑100 (MQ=255)
                                                                                               |                                                                                                
AGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTACGGCTCGGCATCCGGGTCGGATTGTACGCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCA  >  NC_000913/3568466‑3568657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A