Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,722,319	A→C	Q789H (CAA→CAC)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,722,319	0	A	C	99.1%	829.1 / ‑5.0	233	Q789H (CAA→CAC)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (125/106); total (126/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT  >  NC_000913/2722222‑2722414

gCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCacac                                                                                               >  2:2445865/1‑100 (MQ=255)

 cGAGATCAACGAATTTACCCATGAAGATTTAGCCATCATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACg                                                                                              >  2:5353578/1‑100 (MQ=255)

  gagaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACgg                                                                                             <  2:2921595/100‑1 (MQ=255)

    gaTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGaa                                                                                           <  1:659141/100‑1 (MQ=255)

     aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAag                                                                                          <  1:5231193/100‑1 (MQ=255)

     aTCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAag                                                                                          <  1:1987498/100‑1 (MQ=255)

      tCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAaga                                                                                         <  1:3437993/100‑1 (MQ=255)

       cAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAagag                                                                                        <  2:1485521/100‑1 (MQ=255)

        aaCGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAgaga                                                                                       >  2:4127953/1‑100 (MQ=255)

        aaCGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAgaga                                                                                       <  1:4376929/100‑1 (MQ=255)

        aaCGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAgaga                                                                                       >  1:1647981/1‑100 (MQ=255)

         aCGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGAt                                                                                      >  2:4574318/1‑100 (MQ=255)

           ggaTTGACCCATGAAGATTTAGCCAACCTGACACAGATCGACTACGCTCGGGAAGTGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATcc                                                                                    <  2:1096521/98‑1 (MQ=255)

           gAATTTACCCATGAAGATTTAGCCAACTTGACACAGATCGGCTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATcc                                                                                    <  2:5255600/100‑1 (MQ=255)

             aTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTc                                                                                  <  1:3354289/100‑1 (MQ=255)

             aTTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTc                                                                                  >  1:3765125/1‑100 (MQ=255)

              tttACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCg                                                                                 >  2:4841412/1‑100 (MQ=255)

              tttACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAACAGATCCTCg                                                                                 <  2:5335713/100‑1 (MQ=255)

               ttACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCgg                                                                                <  1:642228/100‑1 (MQ=255)

                tACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGc                                                                               >  2:2672890/1‑100 (MQ=255)

                  cccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGt                                                                             >  1:3628825/1‑100 (MQ=255)

                   ccATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTc                                                                            <  1:2205235/100‑1 (MQ=255)

                    cATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCa                                                                           >  1:1256262/1‑100 (MQ=255)

                    cATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCa                                                                           >  2:1861333/1‑100 (MQ=255)

                    cATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATCGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCCCGGCGTCa                                                                           >  2:4287848/1‑100 (MQ=255)

                     aTGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCAc                                                                          >  2:5397402/1‑100 (MQ=255)

                     aTGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCAc                                                                          >  2:1259476/1‑100 (MQ=255)

                     aTGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCAc                                                                          <  2:2042003/100‑1 (MQ=255)

                     aTGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCAc                                                                          <  1:3251481/100‑1 (MQ=255)

                      tGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCAcg                                                                         >  1:2758370/1‑100 (MQ=255)

                       gAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGCTCCTCGGCGTCAcgc                                                                        >  2:3255671/1‑100 (MQ=255)

                        aaGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCAcgcg                                                                       >  1:5464228/1‑100 (MQ=255)

                          gATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTg                                                                     <  1:5075582/100‑1 (MQ=255)

                            tttAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCg                                                                   <  1:3129845/100‑1 (MQ=255)

                               aGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGAtt                                                                <  2:4734513/100‑1 (MQ=255)

                                  cAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTcc                                                             <  2:3462832/100‑1 (MQ=255)

                                   aaCATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCg                                                            >  2:1181185/1‑100 (MQ=255)

                                   aaCATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCg                                                            >  1:1103583/1‑100 (MQ=255)

                                      aTGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATc                                                         <  1:2299382/100‑1 (MQ=255)

                                       tGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATcc                                                        <  1:1005684/100‑1 (MQ=255)

                                       tGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATcc                                                        <  1:2392663/100‑1 (MQ=255)

                                       tGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATcc                                                        >  2:2946281/1‑100 (MQ=255)

                                          cacaGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGa                                                     >  2:2237169/1‑100 (MQ=255)

                                            taGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGAtn                                                   <  1:4439560/99‑2 (MQ=255)

                                            caGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATa                                                   >  2:1332232/1‑100 (MQ=255)

                                              gATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAc                                                 <  2:3376648/100‑1 (MQ=255)

                                              gATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAc                                                 >  1:845249/1‑100 (MQ=255)

                                              gATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAc                                                 >  1:1021443/1‑100 (MQ=255)

                                              gATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAAc                                                 >  1:3379099/1‑100 (MQ=255)

                                                  gACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCg                                             >  1:2726305/1‑100 (MQ=255)

                                                  gACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCg                                             <  1:3552269/100‑1 (MQ=255)

                                                  gACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCg                                             >  1:4529054/1‑100 (MQ=255)

                                                   aCTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                            >  2:3201567/1‑100 (MQ=255)

                                                   aCTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGa                                            >  1:5325861/1‑100 (MQ=255)

                                                     tACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATg                                          >  1:1813824/1‑100 (MQ=255)

                                                      aCGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGc                                         >  1:1892383/1‑100 (MQ=255)

                                                       cGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                        >  1:1461559/1‑100 (MQ=255)

                                                       cGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                        >  1:3958707/1‑100 (MQ=255)

                                                       cGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                        >  1:3870219/1‑100 (MQ=255)

                                                       cGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGcc                                        >  1:1149523/1‑100 (MQ=255)

                                                        gATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGTCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCg                                       <  2:5234528/100‑1 (MQ=255)

                                                         aTCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGa                                      >  2:5102147/1‑100 (MQ=255)

                                                           cGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAt                                    <  2:1975912/100‑1 (MQ=255)

                                                           cGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAt                                    >  1:24372/1‑100 (MQ=255)

                                                           cGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAt                                    >  1:2223189/1‑100 (MQ=255)

                                                             ggAAATGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAAttt                                  <  1:2541142/100‑1 (MQ=255)

                                                               aaaTGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGc                                <  1:4910412/100‑1 (MQ=255)

                                                                aaTGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCt                               >  2:5496912/1‑100 (MQ=255)

                                                                  tGGCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGt                             <  2:330616/100‑1 (MQ=255)

                                                                   ggCGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTa                            >  2:4995160/1‑100 (MQ=255)

                                                                     cGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTAcn                          <  1:2110116/100‑2 (MQ=255)

                                                                     cGTTTGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACt                          <  1:4165545/100‑1 (MQ=255)

                                                                         tGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTTGtt                      <  1:605139/100‑1 (MQ=255)

                                                                         tGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGtt                      <  1:292465/100‑1 (MQ=255)

                                                                         tGTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGtt                      >  1:5050739/1‑100 (MQ=255)

                                                                         tGTAGCGGTACGACGTATTGATCACACGGAAGAGAGCCTCGGCGTGACGCGTGCGATTTCCGATCCTGATCACATCGATGCCGAATTTGCTGTACTGGtt                      >  2:883870/1‑100 (MQ=255)

                                                                          gTAGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGAGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTc                     <  2:809181/100‑1 (MQ=255)

                                                                            aGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGc                   >  2:3788647/1‑100 (MQ=255)

                                                                            aGCGGTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGc                   >  2:4044809/1‑100 (MQ=255)

                                                                               ggTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCg                <  1:307769/100‑1 (MQ=255)

                                                                               ggTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCg                >  2:5036449/1‑100 (MQ=255)

                                                                                gTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCgg               <  1:4102998/100‑1 (MQ=255)

                                                                                gTACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCgg               >  1:960786/1‑100 (MQ=255)

                                                                                 tACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGa              <  1:5426534/100‑1 (MQ=255)

                                                                                 tACGACGTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGa              <  2:2700126/100‑1 (MQ=255)

                                                                                  acgacgTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAt             >  2:4471019/1‑100 (MQ=255)

                                                                                  acgacgTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAt             >  2:5277361/1‑100 (MQ=255)

                                                                                    gacgTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtct           <  2:4071823/100‑1 (MQ=255)

                                                                                     acgTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGAtctc          <  2:3943116/100‑1 (MQ=255)

                                                                                      cgTATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCa         <  2:642452/100‑1 (MQ=255)

                                                                                        tATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaaa       >  1:5520054/1‑100 (MQ=255)

                                                                                        tATTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACACCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCaaa       <  2:2633276/100‑1 (MQ=255)

                                                                                         aTTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg      <  2:692990/100‑1 (MQ=255)

                                                                                         aTTGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAg      <  2:1771216/100‑1 (MQ=255)

                                                                                           tGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAggg    <  2:4965719/100‑1 (MQ=255)

                                                                                           tGATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAggg    >  2:4450399/1‑100 (MQ=255)

                                                                                            gATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt   >  1:405731/1‑100 (MQ=255)

                                                                                            gATCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGt   <  2:2477835/100‑1 (MQ=255)

                                                                                             aTCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt  >  2:3143897/1‑100 (MQ=255)

                                                                                             aTCACACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGtt  >  1:1634504/1‑100 (MQ=255)

GCGAGATCAACGAATTTACCCATGAAGATTTAGCCAACATGACACAGATCGACTACGATCGGGAAATGGCGTTTGTAGCGGTACGACGTATTGATCAAACGGAAGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTT  >  NC_000913/2722222‑2722414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A