Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,969,899 A→C T80P (ACT→CCT)  mutH → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,969,8990AC99.1% 835.3 / ‑4.0 230T80P (ACT→CCT) mutHmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (126/102);  total (128/102)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 233 total aligned reads displayed.

AGGCTGGATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAAACTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAACCAGCC  >  NC_000913/2969805‑2969995
                                                                                              |                                                                                                
 ggCTGGATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATcc                                                                                            >  1:1072234/1‑100 (MQ=255)
  gCTGGATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATccc                                                                                           >  1:5363516/1‑100 (MQ=255)
  gCTGGATTGGCGTGTTACTGGAGATCTGGCTAGGTACCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATccc                                                                                           <  1:1634028/100‑1 (MQ=255)
     ggATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCtgt                                                                                        >  2:2709252/1‑100 (MQ=255)
     ggATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCtgt                                                                                        >  1:4375829/1‑100 (MQ=255)
      gATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCtgtn                                                                                       <  1:4438924/100‑2 (MQ=255)
      gATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCtgtg                                                                                       <  1:3090518/100‑1 (MQ=255)
      gATTGGCGTGTTACGGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCtgtg                                                                                       <  2:3709742/100‑1 (MQ=255)
      aaTTGGCGTGTTACTGGAGATCTGGGTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCGGGGCGTGGTACTTAAACCTATCCCtgtg                                                                                       <  2:3490667/99‑1 (MQ=255)
         tGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGAt                                                                                    <  2:1058045/100‑1 (MQ=255)
          ggCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATa                                                                                   >  1:864078/1‑100 (MQ=255)
           gCGTGTTACTGGAGATCTGGCTAGGTGCCAGCACAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAg                                                                                  >  2:3291573/1‑100 (MQ=255)
             gtgtTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTc                                                                                >  2:778327/1‑100 (MQ=255)
              tgtTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCt                                                                               >  2:3747276/1‑100 (MQ=255)
                 tACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTgg                                                                            <  2:1513158/100‑1 (MQ=255)
                  aCTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGgt                                                                           <  2:1383428/100‑1 (MQ=255)
                  aCTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGgt                                                                           >  1:1150798/1‑100 (MQ=255)
                    tGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGgtcg                                                                         <  1:3463779/100‑1 (MQ=255)
                        gaTCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCg                                                                     >  2:2731738/1‑100 (MQ=255)
                        gaTCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCg                                                                     >  1:3960376/1‑100 (MQ=255)
                           cTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTg                                                                  >  2:4367830/1‑100 (MQ=255)
                           cTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCCCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTg                                                                  >  1:4251329/1‑100 (MQ=255)
                            tGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCTCTGTGGATTGCCTTGGTGGTCGCCTgg                                                                 >  2:2273620/1‑100 (MQ=255)
                             ggCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGa                                                                <  2:1622552/100‑1 (MQ=255)
                             ggCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGa                                                                >  2:3958109/1‑100 (MQ=255)
                              gCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGaa                                                               <  2:404688/100‑1 (MQ=255)
                               cTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATCGTCTTGGTCGTCCGCTGGaaa                                                              >  2:2171007/1‑100 (MQ=255)
                               cTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGaaa                                                              >  1:3698031/1‑100 (MQ=255)
                                  ggTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAaacaa                                                           >  2:285001/1‑100 (MQ=255)
                                  ggTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAaacaa                                                           >  1:1968688/1‑100 (MQ=255)
                                  ggTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAaacaa                                                           >  2:2059489/1‑100 (MQ=255)
                                    tGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAacaaca                                                         >  2:57859/1‑100 (MQ=255)
                                    tGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAacaaca                                                         >  1:4409886/1‑100 (MQ=255)
                                    tGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAacaaca                                                         >  1:3189605/1‑100 (MQ=255)
                                     gCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACAt                                                        >  2:255679/1‑100 (MQ=255)
                                     gCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACAt                                                        >  2:1694165/1‑100 (MQ=255)
                                      ccAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACAtt                                                       >  1:4079670/1‑100 (MQ=255)
                                       cAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTc                                                      >  1:2456002/1‑100 (MQ=255)
                                        aGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCg                                                     >  1:4064759/1‑100 (MQ=255)
                                        aGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCg                                                     >  2:2785907/1‑100 (MQ=255)
                                        aGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCg                                                     >  2:3457341/1‑100 (MQ=255)
                                         gcgcAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGt                                                    <  2:1616521/100‑1 (MQ=255)
                                          cgcAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGtt                                                   >  1:1788006/1‑100 (MQ=255)
                                          cgcAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGtt                                                   <  2:364675/100‑1 (MQ=255)
                                           gcAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGttt                                                  <  2:4693127/100‑1 (MQ=255)
                                            cAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTtg                                                 <  2:3099335/100‑1 (MQ=255)
                                                gAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGtt                                             >  1:2416799/1‑100 (MQ=255)
                                                gAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGtt                                             <  2:1468987/100‑1 (MQ=255)
                                                 aGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTg                                            >  2:4761808/1‑100 (MQ=255)
                                                 aGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTg                                            >  2:1952393/1‑100 (MQ=255)
                                                 aGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTg                                            <  2:1976057/100‑1 (MQ=255)
                                                 aGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTg                                            <  1:3322416/100‑1 (MQ=255)
                                                   tAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGcc                                          <  1:5160205/100‑1 (MQ=255)
                                                    aaaCCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGccc                                         >  2:2600536/1‑100 (MQ=255)
                                                    aaaCCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGccc                                         <  2:3387342/100‑1 (MQ=255)
                                                     aaCCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGcccc                                        >  1:3976692/1‑100 (MQ=255)
                                                          gAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTaa                                   >  1:133771/1‑100 (MQ=255)
                                                           aGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAAc                                  <  1:2263603/100‑1 (MQ=255)
                                                           aGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAAc                                  <  2:4190238/100‑1 (MQ=255)
                                                            gCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACg                                 >  1:2795426/1‑100 (MQ=255)
                                                            gCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACg                                 <  1:3805948/100‑1 (MQ=255)
                                                              aaGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACggg                               <  1:3020909/100‑1 (MQ=255)
                                                              aaGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCACTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACggg                               <  1:3928265/100‑1 (MQ=255)
                                                               aGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGc                              <  2:852706/100‑1 (MQ=255)
                                                               aGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGc                              >  2:5061287/1‑100 (MQ=255)
                                                               aGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGc                              <  2:441657/100‑1 (MQ=255)
                                                               aGATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGc                              <  2:2769917/100‑1 (MQ=255)
                                                                gATTTTGCTGCTCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCa                             <  1:2358877/100‑1 (MQ=255)
                                                                       ctgctCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCgg                      <  1:3387558/100‑1 (MQ=255)
                                                                       ctgctCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCgg                      >  1:461885/1‑100 (MQ=255)
                                                                       ctgctCTGGGCGTGGAACTTAAAACTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCgg                      >  1:4726927/1‑100 (MQ=255)
                                                                         gctGTGGGCGTGGAACTTCAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCgggg                    <  2:1859053/100‑1 (MQ=255)
                                                                         gctCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCgggg                    <  2:3884760/100‑1 (MQ=255)
                                                                         gctCTGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCgggg                    <  1:1517580/100‑1 (MQ=255)
                                                                           tctGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTg                  >  1:778161/1‑100 (MQ=255)
                                                                            ctGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGa                 >  1:1006059/1‑100 (MQ=255)
                                                                            ctGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATGCGTTTGTGTTGCCCCGTTAACGGGCAATAACGGGGTGa                 >  1:5314852/1‑100 (MQ=255)
                                                                            ctGGGCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTAGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGTAATAGCGGGGTGa                 >  1:5023458/1‑100 (MQ=255)
                                                                              gggCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGAcc               <  2:2416991/100‑1 (MQ=255)
                                                                              gggCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTGAACGGGCAATAGCGGGGTGAcc               <  2:5238686/100‑1 (MQ=255)
                                                                                gCGTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTg             <  2:4473185/100‑1 (MQ=255)
                                                                                gCGTGGAACTTAAACCTATCCCTGTGGACAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTg             <  1:2264872/100‑1 (MQ=255)
                                                                                  gTGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTggg           >  2:2698361/1‑100 (MQ=255)
                                                                                   tGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGa          <  1:3236900/100‑1 (MQ=255)
                                                                                   tGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGa          >  2:2524377/1‑100 (MQ=255)
                                                                                   tGGAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGa          >  1:568804/1‑100 (MQ=255)
                                                                                    ggAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGaa         >  2:2583235/1‑100 (MQ=255)
                                                                                    ggAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGCGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGaa         <  2:4231923/100‑1 (MQ=255)
                                                                                    cgatcTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGaa         <  2:4860617/96‑1 (MQ=255)
                                                                                     gAACTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGaaa        <  1:2837530/100‑1 (MQ=255)
                                                                                      aaCTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAAc       >  1:4364050/1‑100 (MQ=255)
                                                                                      aaCTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAAc       >  2:3265625/1‑100 (MQ=255)
                                                                                      aaCTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAAc       >  2:527985/1‑100 (MQ=255)
                                                                                      aaCTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAAc       >  1:1101658/1‑100 (MQ=255)
                                                                                       aCTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAAcc      >  2:1229535/1‑100 (MQ=255)
                                                                                        cTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAACCa     >  1:2613353/1‑100 (MQ=255)
                                                                                        cTTAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAACCa     >  1:1838838/1‑100 (MQ=255)
                                                                                          tAAACCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGaaagatcg   >  1:4929777/1‑95 (MQ=255)
                                                                                           aaacCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAACCAGcc  >  2:3983793/1‑100 (MQ=255)
                                                                                           aaacCTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAACCAGcc  >  1:216662/1‑100 (MQ=255)
                                                                                              |                                                                                                
AGGCTGGATTGGCGTGTTACTGGAGATCTGGCTAGGTGCCAGCGCAGGGAGTAAACCTGAGCAAGATTTTGCTGCTCTGGGCGTGGAACTTAAAACTATCCCTGTGGATAGTCTTGGTCGTCCGCTGGAAACAACATTCGTTTGTGTTGCCCCGTTAACGGGCAATAGCGGGGTGACCTGGGAAACCAGCC  >  NC_000913/2969805‑2969995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A