Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F406 I3 R1	15	135.2	6912603	92.5%	6394157	99.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,470	A→G	M385V (ATG→GTG)	yfjW →	CP4‑57 prophage; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,470	0	A	G	98.3%	418.1 / ‑2.9	121	M385V (ATG→GTG)	yfjW	CP4‑57 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (70/48); total (73/48)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 123 total aligned reads displayed.

ATGATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAGA  >  NC_000913/2774378‑2774564

aTGATCAAACACACGACATAATAATAAATGATAAAAAAAATAAAATAGAAAATCATCTTGCACATGAATGTAGGCACCATAAAAATAAGAAGCTGAAgt                                                                                          >  2:836739/1‑99 (MQ=255)

 tGATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtg                                                                                         <  1:1055444/99‑1 (MQ=255)

  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:237190/99‑1 (MQ=255)

  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:643639/99‑1 (MQ=255)

  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:1918604/99‑1 (MQ=255)

  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:1894449/99‑1 (MQ=255)

  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        <  1:149444/99‑1 (MQ=255)

  gATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATAATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAgtgt                                                                                        >  2:3068131/1‑99 (MQ=255)

   aTTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGtgtg                                                                                       <  1:2612896/99‑1 (MQ=255)

    ttATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAACAATAAGAAGGTGAAGTGTGa                                                                                      >  2:2439678/1‑99 (MQ=255)

    ttATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGa                                                                                      >  2:1782103/1‑99 (MQ=255)

      atacgtacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATAATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAAt                                                                                    <  1:3120505/99‑1 (MQ=255)

      atacatacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTACACCTCTATTTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAAt                                                                                    >  2:2307635/1‑99 (MQ=255)

       tacatacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATa                                                                                   >  2:853438/1‑99 (MQ=255)

        acatacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATAc                                                                                  <  1:1762002/99‑1 (MQ=255)

          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                <  1:1378531/99‑1 (MQ=255)

          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                >  2:2622549/1‑99 (MQ=255)

          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                >  2:2049284/1‑99 (MQ=255)

          atacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCa                                                                                >  2:2041928/1‑99 (MQ=255)

           tacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAg                                                                               >  1:1020159/1‑99 (MQ=255)

           tacGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAg                                                                               >  1:1574082/1‑99 (MQ=255)

            acGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGc                                                                              <  1:2430145/99‑1 (MQ=255)

            acGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACAAGc                                                                              >  2:1120848/1‑99 (MQ=255)

              gATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCaa                                                                            <  1:543125/99‑1 (MQ=255)

              gATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCaa                                                                            >  1:520819/1‑99 (MQ=255)

              gATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGAACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCaa                                                                            >  1:3114723/1‑99 (MQ=255)

                tataATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAat                                                                          >  1:1513137/1‑99 (MQ=255)

                 ataataataAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAata                                                                         >  1:1131245/1‑99 (MQ=255)

                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       >  2:216713/1‑99 (MQ=255)

                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       >  1:3176029/1‑99 (MQ=255)

                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       >  1:2181378/1‑99 (MQ=255)

                   aataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGAAAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATc                                                                       <  1:3039204/99‑1 (MQ=255)

                    ataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCg                                                                      >  1:1358772/1‑99 (MQ=255)

                    ataataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCg                                                                      >  2:2651562/1‑99 (MQ=255)

                     taataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACTTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCgg                                                                     >  1:617163/1‑99 (MQ=255)

                      aataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGACGTGTGAATACCAGCAAATATCGGa                                                                    <  1:3112868/99‑1 (MQ=255)

                       ataaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGAt                                                                   >  1:2856419/1‑99 (MQ=255)

                        taaATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGAtt                                                                  >  1:3051321/1‑99 (MQ=255)

                          aaTGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTa                                                                >  1:1679923/1‑99 (MQ=255)

                           aTGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTaa                                                               >  2:1519551/1‑99 (MQ=255)

                            tGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAAc                                                              <  1:1896363/99‑1 (MQ=255)

                               tAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACaaa                                                           >  2:623216/1‑99 (MQ=255)

                                 aaaaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATa                                                         >  2:792739/1‑99 (MQ=255)

                                  aaaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATAc                                                        <  1:1017565/99‑1 (MQ=255)

                                  aaaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATAc                                                        >  1:1249347/1‑99 (MQ=255)

                                   aaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACg                                                       >  1:1092792/1‑99 (MQ=255)

                                   aaaTATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACg                                                       >  1:2693570/1‑99 (MQ=255)

                                      tataAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGtat                                                    <  1:1964351/99‑1 (MQ=255)

                                       ataAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGtata                                                   >  1:1233047/1‑99 (MQ=255)

                                         aaaaTTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAAc                                                 <  1:2924008/99‑1 (MQ=255)

                                           aaTTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGCAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAAAAAATACGTCTAatat                                               >  1:1735723/1‑99 (MQ=255)

                                           aaTTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACACGCAAATATCGGATTTAACAAATACGTATAACAt                                               >  2:1266918/1‑99 (MQ=255)

                                             ttGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTa                                             >  2:3148287/1‑99 (MQ=255)

                                             ttGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTa                                             >  2:2463695/1‑99 (MQ=255)

                                             ttGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTa                                             >  2:491186/1‑99 (MQ=255)

                                               gAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAAt                                           >  1:3003998/1‑99 (MQ=255)

                                                aaaaTCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATg                                          >  2:831872/1‑99 (MQ=255)

                                                    tCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCaaa                                      <  1:1588787/99‑1 (MQ=255)

                                                    tCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCaaa                                      <  1:954208/99‑1 (MQ=255)

                                                     cATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAAt                                     <  1:1690711/99‑1 (MQ=255)

                                                      aTGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAAtt                                    <  1:2290066/99‑1 (MQ=255)

                                                        gTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGTCTGTAACAAATACGTAAAACATTAATGCAAATTag                                  >  2:1487577/1‑98 (MQ=255)

                                                        gTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTAc                                  <  1:1206223/99‑1 (MQ=255)

                                                          tGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACtt                                <  1:2130253/99‑1 (MQ=255)

                                                          tGCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACtt                                >  2:1815485/1‑99 (MQ=255)

                                                           gCACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTa                               <  1:1836172/99‑1 (MQ=255)

                                                            cACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTaa                              <  1:2918088/99‑1 (MQ=255)

                                                            cACCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTaa                              <  1:1642114/99‑1 (MQ=255)

                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             <  1:2072208/99‑1 (MQ=255)

                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             <  1:479731/99‑1 (MQ=255)

                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             <  1:478690/99‑1 (MQ=255)

                                                             aCCTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAAc                             >  2:2785677/1‑99 (MQ=255)

                                                              ccTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACg                            <  1:2490594/99‑1 (MQ=255)

                                                              ccTCTATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACg                            >  1:1057264/1‑99 (MQ=255)

                                                               ctctATCTAGGCTTGATAAAAATAAGAAGGTGAGGGGTGAATACGAGCAAATATCGGATTTAACAAATACGTATGACATTAATGCAAATTACTTAACgg                           >  2:1597625/1‑99 (MQ=255)

                                                               ctctATCTAGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACgg                           >  1:76587/1‑99 (MQ=255)

                                                                  tatctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtt                        <  1:3443854/99‑1 (MQ=255)

                                                                  tatctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtt                        >  2:2294096/1‑99 (MQ=255)

                                                                  tatctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtt                        <  1:1772962/99‑1 (MQ=255)

                                                                   atctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGttt                       >  1:3354790/1‑99 (MQ=255)

                                                                    tctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtttt                      <  1:3143948/99‑1 (MQ=255)

                                                                    tctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGCATTTAACAAATACGTATAACATTGATGCAAATTATTTACCGGGtttt                      >  2:1902470/1‑99 (MQ=255)

                                                                     ctaGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATGTAACAAATACGTATAACATTAATGCAACTTACTTAACGGGtgttt                     >  2:2622087/1‑99 (MQ=255)

                                                                      taGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGtttttt                    >  1:1814016/1‑99 (MQ=255)

                                                                       atgCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACACTAATGCAAATTACTTAACGTGTTTTTTa                   <  2:2819626/97‑1 (MQ=255)

                                                                       aGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTa                   >  1:89920/1‑99 (MQ=255)

                                                                       aGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTa                   >  1:1485626/1‑99 (MQ=255)

                                                                       aGGCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTa                   <  1:1793456/99‑1 (MQ=255)

                                                                        ggCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTAc                  >  1:3032685/1‑99 (MQ=255)

                                                                         gCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACt                 >  2:853731/1‑99 (MQ=255)

                                                                         gCTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAAAATTAATGCAAATTACTTAACGGGTTTTTTACt                 >  2:1385905/1‑99 (MQ=255)

                                                                          cTTGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCTGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACtt                <  1:1638971/99‑1 (MQ=255)

                                                                            tGATAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGt              >  2:104229/1‑99 (MQ=255)

                                                                               tAAAAATAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCt           <  1:1921934/99‑1 (MQ=255)

                                                                                aaaaaTAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTa          >  1:1764245/1‑99 (MQ=255)

                                                                                  aaaTAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTaaa        >  2:356904/1‑99 (MQ=255)

                                                                                   aaTAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTaaaa       >  1:2344845/1‑99 (MQ=255)

                                                                                     tAAGAAGGTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAAcc     >  2:1661217/1‑99 (MQ=255)

                                                                                        gaaggTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAga  <  2:3123141/99‑1 (MQ=255)

                                                                                        gaaggTGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAga  <  1:2010006/99‑1 (MQ=255)

ATGATTATACATACGATATAATAATAAATGATAAAAAATATAAAATTGAAAATCATGTTGCACCTCTATCTAGGCTTGATAAAAATAAGAAGATGAAGTGTGAATACCAGCAAATATCGGATTTAACAAATACGTATAACATTAATGCAAATTACTTAACGGGTTTTTTACTTGTCCTAAAACCAGA  >  NC_000913/2774378‑2774564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A