Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F55 I0 R1 26 48.4 1146576 93.4% 1070901 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA100.0% 196.2 / ‑6.5 71A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (32/34);  total (34/37)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279570‑2279841
                                                                                                                                       |                                                                                                                                        
gtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATTCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgt                                                                                                                                      <  2:303570/140‑1 (MQ=255)
  gCCGGTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                    <  1:3424/140‑1 (MQ=255)
   ccGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCAGTTGCATGATCAGTTAGTGAGGTTGCgggtgt                                                                                                                                   >  2:17260/1‑140 (MQ=255)
   ccGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACCGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttgt                                                                                                                                   >  2:288436/1‑140 (MQ=255)
    cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                                  >  1:436631/1‑140 (MQ=255)
     gTTATTAGTTGATTCTTCTTCTGGTTTACTAGGTACATCGTTATCTTTTGTGGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt                                                                                                                                 <  2:265896/140‑1 (MQ=255)
     gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTCGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt                                                                                                                                 >  1:306954/1‑140 (MQ=255)
     gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTCCATCCTCCGTTGCTGCTCTTCCtgttgctc                                                                                                                                 >  1:111058/1‑137 (MQ=255)
      ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGtttt                                                                                                                                >  1:236339/1‑139 (MQ=255)
      ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc                                                                                                                                <  1:313295/140‑1 (MQ=255)
           aGTTGATTCTACTTCTGGTTTATTAGTTACTTCGTTATCCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAAGTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg                                                                                                                           <  2:567755/140‑1 (MQ=255)
           aGTTGATTCGTCTTCTGGTTTACTTGTTACATCGTTATCCGTTGTCGGACTGTTTGTTGATGCATCTACACTAGATTTTTTGTTAGCTTGCTGTTTATTATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg                                                                                                                           <  2:290049/140‑1 (MQ=255)
            gTTGATTCTACTTCTGGTTTACTGGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTCTTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt                                                                                                                          >  1:376074/1‑140 (MQ=255)
                aTTCTACTTCTTGTTTACTAGTTACATCGTTATCCATTTTCTTACTGTTTGTTGATTCATCTACACTAGAATTGTTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTTATGTTACTGTTGTTTCACCTGTTGcc                                                                                                                      <  2:211231/140‑1 (MQ=255)
                 ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCg                                                                                                                     <  2:242615/140‑1 (MQ=255)
                  tcttctTGTGGTTTACTAGTTACATCGTTATCCATTGTCGGGCGGTTTGTTGACTCATCTACACTAGAGTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                    <  2:254749/140‑1 (MQ=255)
                    tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAt                                                                                                                  >  1:209598/1‑140 (MQ=255)
                     aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                                 >  1:206425/1‑140 (MQ=255)
                     aCTTCTGGTTTACTAGTTACACTGTTGTAGATTGTTGGATTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTAATGTTGTTTCATCTGTTGCTGCATc                                                                                                                 >  2:468569/1‑140 (MQ=255)
                       ttCTGGTTGACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTTCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               <  1:22040/140‑1 (MQ=255)
                        tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                              <  2:262455/140‑1 (MQ=255)
                        tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                              <  1:336104/140‑1 (MQ=255)
                        gcTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTCCACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                              <  2:157467/139‑1 (MQ=255)
                                aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                      >  2:146081/1‑140 (MQ=255)
                                aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                      >  1:502025/1‑140 (MQ=255)
                                  tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                    <  1:375151/140‑1 (MQ=255)
                                  tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt                                                                                                    <  1:261391/140‑1 (MQ=255)
                                    gTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtgt                                                                                                  >  1:166619/1‑140 (MQ=255)
                                                 ccgttGTCGGACTGTCTGTTGCTGCCTCTACACTAGCATTGTTATTAGCTTGCGGTTTCTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTGTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                     <  1:907/137‑1 (MQ=255)
                                                 ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCg                                                                                     <  1:84741/140‑1 (MQ=255)
                                                  cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTTATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTTGTGTTGTCGGAGAAGCGt                                                                                    <  2:122092/140‑1 (MQ=255)
                                                  cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGCAGCGt                                                                                    >  1:321170/1‑140 (MQ=255)
                                                   gttGTCGGTCTGTTTGGTGATGCATCGACGCTTGGATTGTTATTTGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTCTGTGTTGTGTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                   <  2:377472/139‑1 (MQ=255)
                                                   aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTCTTATTTAGTGTTTTCTGACAAGcacc                                                                                   >  2:490149/1‑137 (MQ=255)
                                                   aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGCAGCGTc                                                                                   >  1:321783/1‑140 (MQ=255)
                                                            cggtttgttGCTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGcc                                                                          <  1:437149/138‑1 (MQ=255)
                                                               tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAtt                                                                       >  1:27219/1‑140 (MQ=255)
                                                                  gttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                    <  1:137004/140‑1 (MQ=255)
                                                                        gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATGACTATTATTTGGTGTGGTCGGAGAAGCGTCCGCTTTGCCATTAGCGgtcgt                                                              >  2:16012/1‑140 (MQ=255)
                                                                        gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTATCTgtggt                                                              >  1:183763/1‑140 (MQ=255)
                                                                        gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTCCTTTGCCATTAGCTgtcgt                                                              >  2:411008/1‑140 (MQ=255)
                                                                          aTCTACACTTGAATTGTTGTTAGCTTGCGGTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct                                                            <  2:443227/140‑1 (MQ=255)
                                                                           tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc                                                           >  2:318567/1‑140 (MQ=255)
                                                                           tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc                                                           >  1:275927/1‑140 (MQ=255)
                                                                               cacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCCTTAGCTGTCGTCTCAgct                                                       >  1:127370/1‑138 (MQ=255)
                                                                                acTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGACGCGTCTGCTTTGCCTTTAGCCGTCGTCTCAGATa                                                      >  2:401949/1‑140 (MQ=255)
                                                                                acTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTCCCATTAGCTGCCGgctcggtta                                                      >  2:56207/1‑135 (MQ=255)
                                                                                       ttGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGTAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                               <  2:209598/140‑1 (MQ=255)
                                                                                        tGTTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTCGTTTTACTGCTGTTTCGCATGTTGCCGCCTCATTTTTATGTGGTGGTGTCGGAGAAGCGTCTGCTTTGTCATTTGCTGTCGTCTCAGATACGTTAGGt                                              <  2:10126/140‑1 (MQ=255)
                                                                                         gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGtt                                             >  2:506423/1‑140 (MQ=255)
                                                                                               aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGACGGTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg                                       <  2:553771/140‑1 (MQ=255)
                                                                                                gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTCGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGCCGTCTCACATCCGTTAGGTTGTCCAGt                                      >  1:568530/1‑140 (MQ=255)
                                                                                                gCTTGCGGTTTATCAGTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGt                                      <  1:41498/140‑1 (MQ=255)
                                                                                                    gCGGTTTATCATTTGCATCATCAGTTTTTTATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTATCTGTGGTCTCAGATACGTTAGGTTGTCCAGTAttt                                  >  2:317782/1‑140 (MQ=255)
                                                                                                     cGGTTTATCTTTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt                                 <  1:169262/140‑1 (MQ=255)
                                                                                                      ggTTTTTCCTTTGCATCATCGGTTGCTGATGTTACTGGTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  2:455394/140‑1 (MQ=255)
                                                                                                      ggTTTATCATTTGCATCAGCGGTTGCTGATGTTACTGTTGTTTGACCTGTTGCCGCATCACGATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCGAGTATTTTc                                <  2:192450/140‑1 (MQ=255)
                                                                                                       gTTTATCATTTCCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                               <  2:67316/140‑1 (MQ=255)
                                                                                                        tttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                              >  1:178853/1‑140 (MQ=255)
                                                                                                         ttAGCATTTGCATCATCAGTGGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                             <  1:386451/140‑1 (MQ=255)
                                                                                                          tATCATTCGCATCATCAGTTGCGCATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                            <  1:137882/140‑1 (MQ=255)
                                                                                                           aTCATTTGCATCATCGGTTGCTGATGTTACGGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtg                           <  2:568942/140‑1 (MQ=255)
                                                                                                           aTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCCCATCACTATTATTTGGTGTTGTCGTAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtg                           >  2:286273/1‑140 (MQ=255)
                                                                                                            tCATTTGCGTCATCAGTTGCTGATGTTAGTGTTGTTTCACCGGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                          <  2:359960/140‑1 (MQ=255)
                                                                                                                 tGCATCATCAGTTGCTGATGTTACTGTTGTATCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGAAGCAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATCCGTTAGGTTGTCCTGCATTTGCTgttgttcgct                     >  1:58711/1‑136 (MQ=255)
                                                                                                                   catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGGGTTGCATTa                   >  2:204917/1‑140 (MQ=255)
                                                                                                                       atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATGATTTGGTGTTGTCGGAGAAGCGGCTGCTTTGCCATTCGCTGTCGTATCAGATACGGGAGGTGGTCCAGGAGAGTATGGTGTTGCAtgggcgc               >  1:48746/1‑134 (MQ=255)
                                                                                                                              gCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt        <  1:539654/140‑1 (MQ=255)
                                                                                                                                 taTGTTACTGTTGGGTCACCGAAGGCCGCAGGACTAGCAGGTGGTTTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCGGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:443470/139‑1 (MQ=255)
                                                                                                                                 gATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTGTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTGCAGTATTTTGTGGTGTTGCATTCGCatttgcatttgc     >  2:89053/1‑140 (MQ=255)
                                                                                                                                    gTTTCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt  <  2:416159/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279570‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A