Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F117 I1 R1	27	126.2	2957298	92.6%	2738457	136.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,289,324	G→T	Q56K (CAG→AAG)	cdaA ←	Cyclic di‑AMP synthase CdaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,289,324	0	G	T	96.0%	331.3 / 3.6	102	Q56K (CAG→AAG)	cdaA	Cyclic di‑AMP synthase CdaA
Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (50/46); total (53/49)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 102 total aligned reads displayed.

AAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTG  >  USA300TCH1516_ALE/2289188‑2289460

aaGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGa                                                                                                                                      >  1:699460/1‑141 (MQ=255)

  gCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAGCGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGAcc                                                                                                                                    >  1:619266/1‑141 (MQ=255)

  gCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGAcc                                                                                                                                    <  1:914131/141‑1 (MQ=255)

  gCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGAcc                                                                                                                                    >  2:611838/1‑141 (MQ=255)

   cTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCa                                                                                                                                   >  4:57456/1‑141 (MQ=255)

        tctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAAc                                                                                                                              >  4:377020/1‑141 (MQ=255)

        tctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAAc                                                                                                                              <  2:674767/141‑1 (MQ=255)

        tctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAAc                                                                                                                              >  4:399175/1‑141 (MQ=255)

            ccAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAAtt                                                                                                                          <  2:381907/141‑1 (MQ=255)

            ccAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAAtt                                                                                                                          <  1:945186/141‑1 (MQ=255)

                 ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTaa                                                                                                                     <  2:692700/141‑1 (MQ=255)

                  tgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAat                                                                                                                    >  4:202753/1‑141 (MQ=255)

                   gttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAata                                                                                                                   >  2:650006/1‑141 (MQ=255)

                      cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTACTATcc                                                                                                                >  4:354482/1‑141 (MQ=255)

                      cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATcc                                                                                                                >  4:404528/1‑141 (MQ=255)

                            cACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTaa                                                                                                          <  1:5799/141‑1 (MQ=255)

                             aCGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTCATACTCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAg                                                                                                         <  1:271556/141‑1 (MQ=255)

                                tCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTaa                                                                                                      >  3:206708/1‑141 (MQ=255)

                                tCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTaa                                                                                                      >  3:107318/1‑141 (MQ=255)

                                 cTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAAt                                                                                                     >  2:262389/1‑141 (MQ=255)

                                 cTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAAt                                                                                                     >  1:786304/1‑141 (MQ=255)

                                 cTAATTTCTAGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAAAGATATAGAATAATTAAAATATTGCAGTCAAATTCAATATCATACTTATCTTCTGACCAATAACAAATACTAATATCACTATAaataat                                                                                                     >  2:596840/1‑141 (MQ=255)

                                  tAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAAtt                                                                                                    <  1:650006/141‑1 (MQ=255)

                                  tAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAAtt                                                                                                    >  3:262524/1‑141 (MQ=255)

                                  tAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAAtt                                                                                                    >  3:262311/1‑141 (MQ=255)

                                   aaTTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTg                                                                                                   >  2:885423/1‑141 (MQ=255)

                                    aTTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGt                                                                                                  <  3:207961/141‑1 (MQ=255)

                                    aTTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGt                                                                                                  >  2:573457/1‑141 (MQ=255)

                                        cTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCg                                                                                              <  3:216639/141‑1 (MQ=255)

                                        cTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTAAATATAATAATTATATTTTGCCTAATCTCTATCATTGATATCCCGTTAAGTAATTGTATCg                                                                                              >  1:970782/1‑141 (MQ=255)

                                         tGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                                                                                             >  1:215665/1‑141 (MQ=255)

                                         tGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                                                                                             >  1:215670/1‑141 (MQ=255)

                                         tGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                                                                                             <  1:87069/141‑1 (MQ=255)

                                            ttGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                                                                                          >  2:507784/1‑141 (MQ=255)

                                              ggAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAg                                                                                        <  1:531537/141‑1 (MQ=255)

                                               gAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGt                                                                                       <  1:660612/141‑1 (MQ=255)

                                                   atTACTATTAAAGCTAATGCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTccc                                                                                   <  1:680090/141‑1 (MQ=255)

                                                   atTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTccc                                                                                   <  2:712182/141‑1 (MQ=255)

                                                     tACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCACGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCtt                                                                                 >  2:411310/1‑141 (MQ=255)

                                                      aCTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTa                                                                                >  2:531774/1‑141 (MQ=255)

                                                        tATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTaaa                                                                              <  1:206424/141‑1 (MQ=255)

                                                         aTTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAg                                                                             <  1:596624/141‑1 (MQ=255)

                                                            aaaGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGAcc                                                                          <  2:477640/141‑1 (MQ=255)

                                                             aaGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg                                                                         <  4:375507/141‑1 (MQ=255)

                                                             aaGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg                                                                         <  1:6816/141‑1 (MQ=255)

                                                              aGCTAATCCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGt                                                                        <  1:233219/141‑1 (MQ=255)

                                                                  agcaCCCCCCATGGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatg                                                                    <  4:358854/138‑1 (MQ=255)

                                                                  aaTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatg                                                                    >  1:337833/1‑141 (MQ=255)

                                                                  aaTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatg                                                                    >  1:376101/1‑141 (MQ=255)

                                                                   aTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatga                                                                   >  2:19364/1‑141 (MQ=255)

                                                                   aTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatga                                                                   <  2:684627/141‑1 (MQ=255)

                                                                   aTACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtgatga                                                                   <  2:965964/141‑1 (MQ=255)

                                                                    tACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgag                                                                  <  1:657700/141‑1 (MQ=255)

                                                                     aCCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgaga                                                                 >  1:173404/1‑141 (MQ=255)

                                                                        ccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGATCGTGATGagaaga                                                              >  4:373315/1‑141 (MQ=255)

                                                                        ccccATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGagaaga                                                              >  4:44871/1‑141 (MQ=255)

                                                                              tGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGt                                                        >  2:344369/1‑141 (MQ=255)

                                                                               gAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTa                                                       >  2:503408/1‑141 (MQ=255)

                                                                               gAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTa                                                       <  1:35535/141‑1 (MQ=255)

                                                                               gAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTa                                                       <  4:23784/141‑1 (MQ=255)

                                                                               gAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTa                                                       >  1:13572/1‑141 (MQ=255)

                                                                                aaTAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTAc                                                      <  2:895472/141‑1 (MQ=255)

                                                                                 aTAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATGAAGTACa                                                     >  1:653077/1‑141 (MQ=255)

                                                                                   aaCTATATCGAATAATTTAGATGTTGCAGTCAAGTTTAATATCTTACTTATCTTCTTACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATa                                                   <  1:847852/141‑1 (MQ=255)

                                                                                   aaCGATATCGAATAATTTAGATGTTGCAGTCAAGTTTAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATa                                                   <  1:334864/141‑1 (MQ=255)

                                                                                   aaCGATATCGAATAATTTAGATGTTGCAGCCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAAACCGCGATGAGAAGATAAAGTACATa                                                   >  4:176373/1‑141 (MQ=255)

                                                                                    aCGATATCGTATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATAc                                                  <  2:302556/141‑1 (MQ=255)

                                                                                     cGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATAcc                                                 <  1:810070/141‑1 (MQ=255)

                                                                                     cGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATAcc                                                 <  2:699460/141‑1 (MQ=255)

                                                                                         atCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAAc                                             <  2:968260/141‑1 (MQ=255)

                                                                                         atCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAAc                                             >  1:682852/1‑141 (MQ=255)

                                                                                         atCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACAAAAc                                             >  4:57055/1‑141 (MQ=255)

                                                                                          tCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACt                                            <  4:138576/141‑1 (MQ=255)

                                                                                          atgAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACt                                            <  4:212751/139‑1 (MQ=255)

                                                                                              ataatTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTAtta                                        <  4:262311/141‑3 (MQ=255)

                                                                                              ataatTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCa                                        <  4:262524/141‑1 (MQ=255)

                                                                                              ataatTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCa                                        <  1:208825/141‑1 (MQ=255)

                                                                                              ataatTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCa                                        >  1:126235/1‑141 (MQ=255)

                                                                                              ataatGTAGACGTCGCAGACAAGCTCAATAGCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGCGAAGATAACGTACATACCAAACTATCa                                        >  2:183566/1‑141 (MQ=255)

                                                                                                 atTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTa                                     <  2:489485/141‑1 (MQ=255)

                                                                                                     aGATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATc                                 >  1:542238/1‑141 (MQ=255)

                                                                                                     aGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATc                                 >  2:825292/1‑141 (MQ=255)

                                                                                                      gATGTTGCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCa                                >  1:361577/1‑141 (MQ=255)

                                                                                                            gCAGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATa                          >  1:747569/1‑141 (MQ=255)

                                                                                                              aGTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACt                        <  2:786304/141‑1 (MQ=255)

                                                                                                               gTCAAGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTc                       >  4:338998/1‑141 (MQ=255)

                                                                                                                  aaGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATCCTCGtt                    >  2:27401/1‑141 (MQ=255)

                                                                                                                   aGTTCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTa                   >  1:486214/1‑141 (MQ=255)

                                                                                                                     ttCAATATCATACTTACCCCATGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACa                 <  3:114560/141‑1 (MQ=255)

                                                                                                                      tCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATaa                                                           >  1:701740/1‑98 (MQ=255)

                                                                                                                      tCAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATaa                                                           <  2:701740/98‑1 (MQ=255)

                                                                                                                       cAATATCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAAt               >  3:436845/1‑141 (MQ=255)

                                                                                                                         atatCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAAttt             >  4:237705/1‑141 (MQ=255)

                                                                                                                         atatCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAAttt             >  1:985633/1‑141 (MQ=255)

                                                                                                                         atatCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAAttt             <  2:395660/141‑1 (MQ=255)

                                                                                                                          tatCATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAAtttt            <  3:256361/141‑1 (MQ=255)

                                                                                                                             cATACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGTTGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTaa         <  1:411310/141‑1 (MQ=255)

                                                                                                                              aTACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCTCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAAc        <  2:819928/141‑1 (MQ=255)

                                                                                                                               tACTTATCTTCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACg       >  1:532247/1‑141 (MQ=255)

                                                                                                                                    aTCTCCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTg  <  2:317612/141‑1 (MQ=255)

AAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGAAGATAAAGTACATACCAAACTATCAGTAAATCAAGGATACTCGTTACAATTTTTAACGTACTG  >  USA300TCH1516_ALE/2289188‑2289460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A