Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F59 I1 R1
|
23 |
28.6 |
559496 |
98.6% |
551663 |
149.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
USA300TCH1516_ALE |
1,775,913 |
C→T |
G204D (GGT→GAT) |
clpX ← |
ATP‑dependent Clp protease ATP‑binding subunit ClpX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | USA300TCH1516_ALE | 1,775,913 | 0 | C | T | 100.0%
| 71.2
/ NA
| 21 | G204D (GGT→GAT) | clpX | ATP‑dependent Clp protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/11); total (10/11) |
AAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAA > USA300TCH1516_ALE/1775777‑1776057
|
aaaGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCCCCTGaaa > 1:268401/1‑149 (MQ=255)
accaccAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCAc < 1:274371/149‑1 (MQ=255)
ccaAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACgtgt < 2:149767/149‑1 (MQ=255)
caAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGtt < 2:11379/149‑1 (MQ=255)
aaGATATTGGTTGAAAAAATTAGAATCATTTCTTGAACTGTATGTTTGCGTCCACCTTGTGGCGGATCACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGtt > 2:214700/1‑149 (MQ=255)
aGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGttt > 1:121360/1‑149 (MQ=255)
aGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGttt > 1:181996/1‑149 (MQ=255)
gATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGtttt > 1:198283/1‑149 (MQ=255)
aTCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTg < 2:22968/149‑1 (MQ=255)
tGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAAtttt < 2:165460/149‑1 (MQ=255)
gAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTa < 1:231095/149‑1 (MQ=255)
tCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTGTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTc > 2:237063/1‑149 (MQ=255)
cTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCa < 2:252359/149‑1 (MQ=255)
ttGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCAt < 1:132277/149‑1 (MQ=255)
ttGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCAt < 1:214472/149‑1 (MQ=255)
tttGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACataaataa > 2:33524/1‑149 (MQ=255)
cGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTAt < 2:158165/149‑1 (MQ=255)
aaCACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCaa < 1:71930/149‑1 (MQ=255)
tgAACATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTc > 2:72889/1‑149 (MQ=255)
aaCATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATCTTTTCaa > 2:168814/1‑149 (MQ=255)
aaCATCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCaa > 1:240094/1‑149 (MQ=255)
|
AAAGGCACCACCAAGAATAAATAAGATATTTGTTGTATCAATTTGAATCATTTCTTGGTTTGGATGTTTGCGTCCACCTTGTGGCGGAACACTTGCAGTCGTACCTTCTAAGATTTTAAGCAATGCTTGTTGAACACCTTCACCTGAAACGTCACGTGTTATAGATGTGTTTTCAGATTTACGTGCAATTTTATCAATTTCATCTACATAAATAATACCTTTTTCGGCTTTATCAATGTCAAAGTCAGCTGCTTGAATTAATCTCAACAAGATATTTTCAA > USA300TCH1516_ALE/1775777‑1776057
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
