Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 637,959 A→G intergenic (‑163/‑209) dsbG ← / → ahpC periplasmic disulfide isomerase/thiol‑disulphide oxidase/alkyl hydroperoxide reductase, C22 subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb637,9590AG99.6% 666.4 / NA 239intergenic (‑163/‑209)dsbG/ahpCperiplasmic disulfide isomerase/thiol‑disulphide oxidase/alkyl hydroperoxide reductase, C22 subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (0/238);  minor base T (0/1);  total (0/239)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAG  >  W3110S.gb/637918‑637979
                                         |                    
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:34990/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:523543/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:503537/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:488563/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:43333/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:415987/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:410273/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:387877/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:379335/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:374826/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:367229/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:357456/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:53727/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:331212/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:327886/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:291887/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:280744/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:277032/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:264477/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:250341/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:240499/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:200488/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1645024/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:744451/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:988177/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:986124/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:941745/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:923224/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:880198/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:868057/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:838520/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:827219/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:788273/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:765564/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:763368/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:231378/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:728440/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:721919/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:712312/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:704568/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:702684/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:697200/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:662804/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:650372/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:616937/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:552163/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1193607/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1397526/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1378165/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:136358/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1312881/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1307612/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1298281/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1271298/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1219542/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1214371/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1199694/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1336693/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1187388/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1162377/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1156953/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1151234/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1125630/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1119618/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1062778/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1045099/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1028608/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1011903/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:154901/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1584140/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1506199/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1610503/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:161874/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1471829/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:154158/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1543940/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1466985/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:144277/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1427641/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1411503/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:156397/62‑1 (MQ=255)
tgttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1569104/62‑1 (MQ=255)
tgttgCATTCGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:219010/62‑1 (MQ=255)
 gttgTATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1405109/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCATGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:107520/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1510645/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:91375/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1003551/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1626264/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:175678/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:816736/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:448926/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1255939/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1258912/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:69322/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1281995/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:645110/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1634529/61‑1 (MQ=255)
 gttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:461237/61‑1 (MQ=255)
  ttgCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCGCTGAAGATACCAAAGGGTAg  <  1:1401126/60‑1 (MQ=255)
                                         |                    
TGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAG  >  W3110S.gb/637918‑637979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A