Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,099,547 C→T A143V (GCC→GTC)  ycdX → predicted zinc‑binding hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,099,5470CT98.8% 224.3 / NA 80A143V (GCC→GTC) ycdXpredicted zinc‑binding hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (79/0);  minor base A (1/0);  total (80/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAAC  >  W3110S.gb/1099509‑1099570
                                      |                       
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGTAGCCGCGAAAc  >  1:324787/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaaa   >  1:95611/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaaa   >  1:1548069/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaaa   >  1:1545005/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaaa   >  1:89470/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaaa   >  1:329378/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaaa   >  1:437349/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaa    >  1:23746/1‑60 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGaa    >  1:886032/1‑60 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:329270/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:367622/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:353523/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:351510/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:348897/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:886663/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:898829/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:93604/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:45103/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:948231/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:318920/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:289126/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:279969/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:269084/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:256903/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:203252/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:825809/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:512100/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:532647/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:55038/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:565983/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:571140/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:635555/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:688025/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:706599/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:710864/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:738385/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:746586/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:758037/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:758996/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:767689/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:783828/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1098308/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1249507/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1239226/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:117546/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1169999/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1167260/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1141149/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:111998/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1104808/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1100311/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1319651/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1095501/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1093506/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1090969/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1052978/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1052894/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1043283/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:103130/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1030860/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1537164/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:181511/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:178542/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1629791/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1626696/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1592449/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1582824/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1581867/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1538918/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:102981/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:153177/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1465657/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1452713/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1449723/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1442825/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1429267/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1400778/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAAc  >  1:1379193/1‑62 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGACGCGaaa   >  1:1131669/1‑61 (MQ=255)
ccATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGACGTTGCTGAagc             >  1:329591/1‑51 (MQ=255)
                                      |                       
CCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAAC  >  W3110S.gb/1099509‑1099570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A