Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,451,410 |
2 bp→AC |
coding (420‑421/825 nt) |
mepA ← |
murein DD‑endopeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,451,410 | 0 | G | A | 100.0%
| 550.2
/ NA
| 190 | L141L (CTG→TTA) | mepA | murein DD‑endopeptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/190); total (0/190) |
| * | W3110S.gb | 2,451,411 | 0 | T | C | 100.0%
| 559.1
/ NA
| 190 | A140A (GCA→GCG) | mepA | murein DD‑endopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/190); total (0/190) |
Only 100 of 190 total aligned reads displayed.
CAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGT > W3110S.gb/2451377‑2451440
||
cAACGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGC‑‑CGCGGAGGt < 1:707708/62‑1 (MQ=255)
aCGTGTTTACCGTCTCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:133580/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAATCTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:873289/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAggg < 1:827291/62‑2 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAgga < 1:532900/62‑2 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:410507/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:550423/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:543813/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:49527/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:482153/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:47379/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:464473/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:447898/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:441578/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:41806/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:618639/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:394231/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:373311/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:359789/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:353726/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:311886/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:302539/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:278766/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:274245/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:260292/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:237023/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:768368/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:968267/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:955111/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:900702/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:891299/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:862766/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:843394/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:831526/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:807404/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:7865/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:770299/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:598578/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:752709/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:743196/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:722550/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:714144/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:668717/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:666006/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:663778/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:656555/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1020076/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1169750/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1338733/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1331917/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:129981/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1279686/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1273235/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1230713/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1220948/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1200157/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1190639/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1172217/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:188106/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1153836/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1151343/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1142927/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1140801/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1132165/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1091566/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1089771/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1053833/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1042063/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1000616/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1382933/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:207184/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:169034/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1617559/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1601691/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:159557/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1579440/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1574934/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1558920/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1555737/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1527465/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1524241/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1510002/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1488074/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1458985/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1425431/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1416316/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1358439/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1416002/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1400730/62‑1 (MQ=255)
aCGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1388187/62‑1 (MQ=255)
aCGTGTTTACCGCCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:85942/62‑1 (MQ=255)
cGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:99259/61‑1 (MQ=255)
cGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:929936/61‑1 (MQ=255)
cGTGTTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1327067/61‑1 (MQ=255)
cGTGATTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:628577/61‑1 (MQ=255)
gtTTACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:1110619/58‑1 (MQ=255)
tttACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:695982/57‑1 (MQ=255)
tACCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:761690/55‑1 (MQ=255)
aCCGTCGCGGGAAACTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:106512/54‑1 (MQ=255)
aaaCTAAATCTAACGCTTGCGGGCGCAAGAGCTGCGCGGAGGt < 1:869321/43‑1 (MQ=25)
||
CAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGT > W3110S.gb/2451377‑2451440
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
