Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,556,480 G→A A61A (GCC→GCT)  yfeY ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,556,4800GA100.0% 726.3 / NA 252A61A (GCC→GCT) yfeYhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (252/0);  total (252/0)

Only 100 of 252 total aligned reads displayed.

GTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCAA  >  W3110S.gb/2556423‑2556484
                                                         |    
gTTGCCGTTCGCGGTTTTCATTCCGGTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:705389/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:463678/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:728360/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:681822/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:665549/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:656692/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:637291/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:616376/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:544313/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:522621/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:491405/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:473322/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:464757/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:732307/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:417060/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:367359/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:362251/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:333375/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:330084/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:314378/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:303384/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:279493/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:24974/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:244728/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1010504/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:854537/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:986270/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:978732/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:966942/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:947719/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:937963/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:926405/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:919661/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:902800/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:883151/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:877067/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:875338/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:236294/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:845038/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:824474/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:820170/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:81403/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:804869/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:796083/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:793652/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:786738/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:769265/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:758865/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:747763/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1209815/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:212038/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1405011/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1386351/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1374066/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1367837/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1358486/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1342747/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1327681/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1310582/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1268920/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:125741/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1412625/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1179850/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1177622/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1152187/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:111296/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1106168/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:107592/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1068260/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1060428/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1038655/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1034526/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:101808/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1434934/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:197755/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:190233/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:174327/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:166701/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:164736/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1643649/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1634035/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1625903/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1617660/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1612779/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1589406/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1582261/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1568188/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1562591/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1538521/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1530160/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:15205/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1510372/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1494437/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1489194/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1464336/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCa   >  1:1167224/1‑61 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCa   >  1:1084104/1‑61 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGAGCATCAGCaa  >  1:429509/1‑62 (MQ=255)
gTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCAATCAAGCGCATCAGCa   >  1:697110/1‑61 (MQ=255)
gTTGCCGTTAGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCAGCaa  >  1:1380303/1‑62 (MQ=255)
                                                         |    
GTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCAA  >  W3110S.gb/2556423‑2556484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A